全反式维甲酸对NT2细胞系胶质瘤趋向性的影响

目的研究全反式维甲酸(ATRA)对NT2细胞系胶质瘤趋向性的影响。方法将NT2细胞分为4组:NT2组(未经ATRA干预)、NT2RA1周组(ATRA干预1周)、NT2RA2周组(ATRA干预2周)、NT2RA3周组(ATRA干预3周),采用细胞迁移分析的方法,分别检测其对U87胶质瘤细胞、无血清DMEM培养基以及含100mL/L胎牛血清DMEM培养基的趋向性。结果经ATRA干预的NT2细胞,其胶质瘤趋向性较未经干预组(NT2组)显著提高(P<0.05),对非特异性迁移趋化物如血清等的迁移则较未经干预组显著减少(P<0.05),其中NT2RA2周组胶质瘤趋向性最强,与其他各组比较差异有统计学意义(P<0.01)。结论经ATRA干预2周的NT2细胞其胶质瘤特异趋向性显著提高,有望成为临床治疗胶质瘤的新型靶向基因传递载体。

Effects of all-trans retinoid acid on tropism of NT2 cell line for glioma

Objective To investigate the effects of all-trans retinoid acid(ATRA) on tropism of NT2 cell line for glioma.Methods NT2 cells were divided into four groups: NT2 group(untreated NT2 cells),NT2RA1W group(cells treated with ATRA for 1 week),NT2RA2W group(cells treated with ATRA for 2 weeks),and NT2RA3W group(cells treated with ATRA for 3 weeks).Cell migration assay was used to detect the tropism of ATRA-treated NT2 cells for U87 glioma cells,serum-free DMEM and DMEM with 100 mL/L FBS.Results An increase in directional migration of NT2 cells toward glioma was found in each ATRA-treated group(P<0.05) compared with NT2 group.Meanwhile,migration toward other migration-stimulating factors such as FBS was significantly decreased(P<0.05).NT2RA2W group showed the most powerful migration ability toward glioma cells than any other groups(P<0.01).Conclusion NT2 cells treated with ATRA for 2 weeks show significant tropism for U87 glioma cells specially and hold promise to be a novel targeted delivery vehicle for clinical therapy of glioma.