MDR1、BCRP和LRP基因在乳腺癌组织中的表达及其意义

目的探讨多药耐药基因(MDR1)、乳腺癌耐药蛋白(BCRP)及肺耐药蛋白(LRP)mRNA在乳腺癌组织中的表达及其相互关系,分析它们与乳腺癌临床病理特征的关系。方法采用RT-PCR方法检测2007年1月至2007年12月在宁夏医科大学附属医院手术切除的42例乳腺癌组织、42例癌旁组织及50例乳腺良性病变中MDR1、BCRP和LRP mRNA的表达。结果乳腺癌组织中MDR1、BCRP、LRP mRNA的阳性表达率分别为40.47%、38.09%及61.90%,与癌旁组织及乳腺良性病变组织相比阳性表达率均有统计学差异(P<0.05)。MDR1mRNA表达与绝经状况呈完全正相关(r=0.398,P<0.01),与腋窝淋巴结状况呈完全正相关(r=0.398,P<0.01);BCRP mRNA表达与腋窝淋巴结状况呈正相关(r=0.355,P<0.05);LRP mRNA表达与绝经状况、年龄、腋窝淋巴结状况及肿瘤大小之间均无相关性(P>0.05);MDR1mRNA和BCRP mRNA表达呈正相关(r=0.652,P<0.01),LRP mRNA与MDR1mRNA表达无相关性(r=0.147,P>0.05);LRP mRNA和BCRP mRNA表达无相关性(r=0.111,P>0.05)。2个耐药基因共表达率为47.61%,2种或3种耐药基因共表达率为61.89%。结论乳腺癌组织中存在耐药基因MDR1、BCRP和LRP的表达,单基因和多基因协同作用,以多基因共表达为主;检测MDR1和BCRP基因表达水平可辅助临床判断乳腺癌患者的预后。

Expressions of MDR1,BCRP and LRP genes in breast cancer tissues and their significance

Objective To investigate the expressions of multidrug resistance gene(MDR1),breast cancer resistance protein(BCRP) and lung resistance protein(LRP) mRNA in breast cancer and their correlation,and to analyze their relationship with clinical and pathological features of breast cancer.Methods We adopted RT-PCR method to detect the expression levels of MDR1,BCRP and LRPmRNA in 42 cases of breast cancer tissues,42 cases of adjacent tissues and 30 cases of benign breast lesions,who came to the Affiliated Hospital of Ningxia Medical University for resection between January and December 2007.Results The positive expression rate of MDR1 mRNA,BCRP mRNA and LRPmRNA was 40.47%,38.09% and 61.90% in patients with breast cancer.It differed significantly from that in the adjacent tissues and benign breast lesions(P<0.05).The expression of MDR1 mRNA had a completely positive correlation with menopausal status and axillary lymph node status in breast cancer patients(r=0.398,P<0.01).The expression of BCRP mRNA had a positive correlation with axillary lymph node status(r=0.355,P<0.05).The expression of LRP mRNA had no correlation with menopausal status,age,axillary lymph node status or tumor size(P>0.05).The expressions of MDR1 mRNA and BCRP mRNA were positively related(r=0.652,P<0.01),and LRP mRNA expression was not related to the expression of MDR1 mRNA(r=0.147,P>0.05) or BCRP mRNA(r=0.111,P>0.05).The co-expression rate of two resistance genes was 47.61% while that of two or three types of resistance genes was 61.89%.Conclusion Multidrug resistance gene(MDR1),breast cancer resistance protein(BCRP) and lung resistance protein(LRP) mRNA are co-expressed in breast cancer;single gene and multiple genes are synergetic and the co-expression of multiple genes is more important.Detecting the expression of MDR1 and BCRP genes can help determine the prognosis of breast cancer in clinic.