| 人UCA1基因新剪接变异体全长cDNA序列的克隆 |
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| 引用本文： | 王宇,陈葳,李旭.人UCA1基因新剪接变异体全长cDNA序列的克隆[J].西安交通大学学报(医学版),2012,33(1):11-13,28. |
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| 作者姓名： | 王宇 陈葳 李旭 |
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| 作者单位： | 西安交通大学医学院第一附属医院分子医学中心,陕西西安,710061 |
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| 基金项目： | 国家自然科学基金资助项目(No.30973429)~~ |
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| 摘 要： | 目的克隆新的UCA1剪接变异体全长cDNA序列,为研究其可变剪接机制奠定基础。方法用电子克隆技术和cDNA序列末端快速扩增技术(rapid amplification of cDNA ends,RACE)扩增细胞系BLZ-211cDNA并进行产物测序和序列拼接。结果新克隆的UCA1剪接变异体全长cDNA序列为2 202bp。结论综合采用电子克隆技术与RACE技术是获得全长cDNA序列的有效方法,为该基因的后续可变剪接机制的研究奠定了基础。
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| 关 键 词： | UCA1 电子克隆 cDNA末端快速扩增技术 |
Cloning of the full-length cDNA sequence of a novel human UCA1 spliced variant |
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| WANG Yu
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CHEN Wei
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LI Xu.Cloning of the full-length cDNA sequence of a novel human UCA1 spliced variant[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(1):11-13,28. |
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| Authors: | WANG Yu CHEN Wei LI Xu |
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| Institution: | (Center for Molecular Medicine,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061,China) |
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| Abstract: | Objective To clone the full-length cDNA sequence of novel UCA1 spliced isoforms for understanding the exact mechanism of this type of alternative splicing.Methods The full-length cDNA was amplified from BLZ-211 cells by using the in silicon sequence elongation technique,5′-RACE and 3′-RACE techniques.Products of RT-PCR were sequenced and further assembled.Results The new UCA1 spliced isoform sequence was 2 202 bp.Conclusion A combination of the in silicon sequence elongation,5′-RACE and 3′-RACE techniques is an effective way to obtain the full-length cDNA,which will guide further research on the mechanism of this type of alternative splicing. |
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| Keywords: | UCA1 in silicon rapid amplification of cDNA ends(RACE) |
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