生理性衰老过程中大鼠胃肠运动功能与脑-肠轴结构型一氧化氮合酶含量的变化及意义

目的探讨大鼠在生理性衰老过程中胃肠运动功能脑-肠轴结构型一氧化氮合酶含量的变化及其意义。方法将健康SD大鼠按3月龄、9月龄、18月龄和24月龄分为4组,每组各6只。采用双抗体夹心ABC-ELISA法检测大鼠脑-肠轴组织(全脑及胃体、空肠、回肠、结肠、乙状结肠与直肠交界处组织)中的神经型一氧化氮合酶及内皮型一氧化氮合酶含量,并比较其在不同年龄组的变化规律。结果各年龄组大鼠脑-肠轴神经型一氧化氮合酶和内皮型一氧化氮合酶含量随年龄增长而降低,这种变化在成年后(9月龄、18月龄及24月龄)大鼠更为显著(P<0.05,P<0.01)。神经型一氧化氮合酶、内皮型一氧化氮合酶在大鼠脑-肠轴各部位的含量有一定差异,且其分布差异随着大鼠年龄增长无显著性改变。结论在大鼠生理性衰老过程中,脑、胃体、空肠、回肠、结肠、乙状结肠与直肠交界处神经型一氧化氮合酶、内皮型一氧化氮合酶的表达随年龄增长而显著下降,推测老年人功能性胃肠疾病的发病机制可能与此有关。

Changes of nitric oxide synthase in brain-gut axis in the physiological aging process of SD rats

Objective To observe changes of neuronal nitric oxide synthase and endothelial nitric oxide synthase levels in the physiological aging process of SD rats and investigate the possible mechanism of functional gastrointestinal disorders(FGID) in old people.Methods Twenty-four healthy SD rats were divided into four groups at the age of 3 months,9 months,18 months and 24 months,with six rats in each group.The content of neuronal and endothelial nitric oxide synthase in brain-gut axis(brain,gastric body,jejunum,ileum,sigmoid and rectum) was measured by ELISA in each group.Results Level of neuronal and endothelial nitric oxide synthase in brain-gut axis was decreased with aging,and these changes in the adult rats(9 months,18 months and 24 months old) were more significant(P<0.05,P<0.01).The distribution of neuronal and endothelial nitric oxide synthase in brain-gut axis was uneven,and this non-uniform distribution did not change with aging.Conclusion The content of neuronal and endothelial nitric oxide synthase in brain-gut axis of SD rats is decreased in the physiological aging process.These changes may participate in the occurrence and development of functional gastrointestinal disorders in old people.