氧化修饰的低密度脂蛋白对内皮细胞功能的影响

目的　观察OX LDL对内皮细胞NO、ET 1合成及细胞表面细胞间粘附分子 1(ICAM 1)表达的影响。方法　采用培养的人脐静脉内皮细胞 (HUVEC) ,在分别加入不同浓度OX LDL(5 0、10 0、15 0mg·L-1)孵育 2 4h ,或浓度为 10 0mg·L-1于不同时间 (12、2 4、36h)孵育后观察培养液中NO及ET 1水平及细胞表面ICAM 1的表达。结果　OX LDL可显著抑制NO的合成 (P <0 .0 5 ) ,但各时间段培养液中NO含量无显著性差异 (P >0 .0 5 ) ;OX LDL还可明显增加ET 1的分泌及诱导细胞表面ICAM 1的表达。结论　OX LDL对内皮细胞具有明显的细胞毒作用 ,它对NO、ET 1释放及ICAM 1表达的影响可能是其致动脉粥样硬化发生的重要机制。

The functional effect of oxidized low density lipoprotein on endothelial cells

Objective To investigate the effect of oxidized low density lipoprotein on the synthesis of NO, ET-1 and expression of ICAM-1 in human umbilical vein endothelial cells (HUVEC). Methods OX-LDL of 50?mg·L -1, 100?mg·L -1 and 150?mg·L -1 were co-incubated with HUVEC for 24?h in vitro. OX-LDL 100?mg·L -1 were co-incubated with HUVEC for 12 hours, 24 hours or 36 hours respectively. The levels of NO, ET-1 in medium and the surface expression of ICAM-1 on HUVEC were detected after the experiments.Results OX-LDL could significantly inhibit the synthesis of NO (P< 0.05), but at every interval the differences of NO level were not significant (P> 0.05); OX-LDL could increase the secretion of ET-1 and induced ICAM-1 upregulation of surface expression on HUVEC.Conclusion OX-LDL have cytotoxic effects on HUVEC. The influence of OX-LDL on the release of NO, ET-1 and the expression of ICAM-1 on HUVEC may be the crucial mechanism which accounts for its atherogenic effects.