| 恒磁场对人脐动脉血管平滑肌细胞胞浆游离钙离子浓度的影响 |
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| 引用本文： | 冯旭阳,徐瑞芬,宋博,王海昌.恒磁场对人脐动脉血管平滑肌细胞胞浆游离钙离子浓度的影响[J].西安交通大学学报(医学版),2008,29(1):19-21,46. |
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| 作者姓名： | 冯旭阳 徐瑞芬 宋博 王海昌 |
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| 作者单位： | 1. 第四军医大学,西京医院心脏内科,陕西西安,710032
2. 第四军医大学,口腔医院麻醉科,陕西西安,710032 |
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| 摘 要： | 目的 研究恒磁场对培养的人脐动脉血管平滑肌细胞(VSMC)胞浆内游离钙离子浓度(Ca2 ]i)的影响,探讨恒磁场抑制VSMC增殖的作用机制.方法 离体培养人脐动脉VSMC,不同强度的恒磁场垂直作用于VSMC,应用激光共聚焦显微镜观察恒磁场对VSMC胞浆Ca2 ]i的影响.结果 5 mT的恒磁场作用于VSMC后10-50 minCa2 ]i较对照组明显下降,20 min下降最明显;其平滑肌细胞的Ca2 荧光强度明显弱于对照组,至60 min接近对照组.结论 恒磁场对VSMC胞浆Ca2 ]i有明显降低作用,可能是抑制VSMC细胞增殖的机制.为经皮冠状动脉成型术(PTCA)术后再狭窄的治疗提供了新的理论基础.
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| 关 键 词： | 电生理学 游离钙 恒磁场 血管平滑肌细胞(VSMC) 恒磁场 人脐动脉 血管平滑肌细胞 胞浆游离钙离子 浓度的影响 vascular smooth muscle cells umbilical human intracellular free calcium magnetic fields constant 理论 治疗 术后再狭窄 PTCA 经皮冠状动脉成型术 细胞增殖 荧光强度 对照组 磁场作用 |
| 文章编号： | 1671-8259（2008）01-0019-03 |
| 收稿时间： | 2007-04-25 |
| 修稿时间： | 2007-05-23 |
Effect of constant magnetic fields on intracellular free calcium of human umbilical arterial vascular smooth muscle cells |
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| Feng Xuyang,Xu Ruifen,Song Bo,Wang Haichang.Effect of constant magnetic fields on intracellular free calcium of human umbilical arterial vascular smooth muscle cells[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2008,29(1):19-21,46. |
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| Authors: | Feng Xuyang Xu Ruifen Song Bo Wang Haichang |
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| Institution: | Feng Xuyang1,Xu Ruifen2,Song Bo2,Wang Haichang1 |
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| Abstract: | Objective To study effects of constant magnetic fields(CMF) on the intracellular free calcium of human umbilical arterial vascular smooth muscle cells to discuss the mechanism of CMF affecting the proliferation of VSMC.Methods The intracellular free calcium concentration of human umbilical arterial vascular smooth muscle cells was detected by laser scanning confocal microsopy.Results The CMF affected the intracellular free calcium concentration of human umbilical arterial vascular smooth muscle cells.The CMF(5 mT) caused a persistent decrease of intracellular free calcium concentration of VSMC from 10 min to 50 min.Conclusion The CMF could inhibit proliferation of VSMC by decreasing the intracellular free calcium constant magnetic field concentration of VSMC.The CMF could markedly inhibit proliferation of VSMC,which might be useful in treatment of RS after PTCA. |
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| Keywords: | electrophysiology intracellular free calcium constant magnetic fields vascular smooth muscle cells |
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