| 内脂素基因多态性与2型糖尿病的关系 |
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| 引用本文： | 王玉环,闫丽丽,吴娟娟,董鹏,张春虹,王俊宏,丁石梅,张敏.内脂素基因多态性与2型糖尿病的关系[J].西安交通大学学报(医学版),2011,32(4):453-457. |
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| 作者姓名： | 王玉环 闫丽丽 吴娟娟 董鹏 张春虹 王俊宏 丁石梅 张敏 |
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| 作者单位： | 西安交通大学医学院第二附属医院内分泌科,陕西西安,710004 |
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| 基金项目： | 陕西省科学技术研究发展计划项目 |
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| 摘 要： | 目的探讨内脂素(visfatin)rs11977021、rs12537455、rs2110385位点单核苷酸多态性与2型糖尿病易感性的关系。方法收集陕西西安地区汉族人群192例受试对象,其中106例单纯2型糖尿病(T2DM)患者和86例健康对照者,采用标准化的聚合酶链反应-限制性片段长度多态性(PCR-RLFP)方法,分别检测内脂素(visfatin)rs11977021/DdeⅠ、rs12537455/AluⅠ和rs2110385/AluⅠ基因型并进行比较。结果 rs2110385位点的T等位基因频率和GT基因型频率在T2DM组均高于健康对照组(分别为28.3、14.0和14.2、7.0,P均<0.05);两组的rs11977021位点和rs12537455位点C、T等位基因及基因型频率分布均无统计学差异(P>0.05);单倍型分析显示T-C-T和T-T-T单倍型在T2DM组均显著高于健康对照组(分别为0.066、0.026和0.029、0.012,P均<0.05)。结论 Visfatin基因rs2110385位点在陕西西安汉族地区人群中存在遗传变异,其rs2110385多态性位点的T等位基因可能与T2DM的发病风险有一定的关系。
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| 关 键 词： | 内脂素 2型糖尿病 基因 单核苷酸多态性 聚合酶链反应-限制性片段长度多态性(PCR-RFLP) |
Relationship between single nucleotide polymorphisms of visfatin and type 2 diabetes mellitus |
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| WANG Yu-huan,YAN Li-li,WU Juan-juan,DONG Peng,ZHANG Chun-hong,WANG Jun-hong,DING Shi-mei,ZHANG Min.Relationship between single nucleotide polymorphisms of visfatin and type 2 diabetes mellitus[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(4):453-457. |
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| Authors: | WANG Yu-huan YAN Li-li WU Juan-juan DONG Peng ZHANG Chun-hong WANG Jun-hong DING Shi-mei ZHANG Min |
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| Institution: | WANG Yu-huan,YAN Li-li,WU Juan-juan,DONG Peng,ZHANG Chun-hong,WANG Jun-hong,DING Shi-mei,ZHANG Min(Department of Endocrinology,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710004,China) |
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| Abstract: | Objective To investigate the correlation between single nucleotide polymorphisms of rs11977021,rs12537455 and rs2110385 in visfatin gene and type 2 diabetes mellitus(T2DM).Methods A total of 192 Han individuals in Xi'an were selected and divided into two groups: T2DM group consisted of 106 patients while control group consisted of 86 healthy individuals.The anti-coagulated peripheral blood samples were obtained from each individual.DNA was extracted from each blood sample,and single nucleotide polymorphisms... |
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| Keywords: | visfatin type 2 diabetes mellitus gene single nucleotide polymorphism standard polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP) |
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