| 一种通用型重组2型腺伴随病毒载体的构建及外源基因表达的研究 |
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| 引用本文： | 张桐,王文亮,侯云德,杨新科,颜子颖,孙立连,王伯云.一种通用型重组2型腺伴随病毒载体的构建及外源基因表达的研究[J].西安交通大学学报(医学版),1997(3). |
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| 作者姓名： | 张桐 王文亮 侯云德 杨新科 颜子颖 孙立连 王伯云 |
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| 摘 要： | 以野生型2型人腺伴随病毒（AAV－2）基因组载体pSSV9－int-为基础，构建了一种携带和表达真核基因的基础AAV载体pSSV9／MulV－neosv，表达了neo基因，并采用该载体组建了携带人白细胞介素-2cDNA的重组AAV载体pSSV9／MulV－neosv－IL－2，转染A549细胞后表达了人白细胞介素-2基因。为应用腺伴随病毒作基因治疗研究提供了实验基础。
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| 关 键 词： | 腺伴随病毒 载体构建 基因表达 |
CONSTRUCTION OF A UNIVERSAL RECOMBINANT ADENO-ASSOCIATED VIRUS VECTOR AND EXPRESSION OF FOREIGN GENES IN THE VECTOR |
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| Zhang Tong, Wang Wenliang, Hou Yunde et al.CONSTRUCTION OF A UNIVERSAL RECOMBINANT ADENO-ASSOCIATED VIRUS VECTOR AND EXPRESSION OF FOREIGN GENES IN THE VECTOR[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1997(3). |
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| Authors: | Zhang Tong Wang Wenliang Hou Yunde |
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| Abstract: | Based on a vector, pSSV9-int,which bearing the genome of wild type adeno-associ-ated virus type-2, a universal recombinant AAV vector pSSV9/MulV-neosv was constructed. It was used for cloning and expressing enkaryotic genes.The neo gene in the vector was expressed. Further-MulV-neosv-IL-2, which bears a therapeutic gene,human interleukin-2, was constructed. After trans-fecting A549 cells with the plasmid, the cells pro-duced IL-2. This work provided a foundation for the further research in human gene therapy using the rAAV vectors- |
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| Keywords: | adeno-associated virus construction of vectors gene expression |
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