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| 川芎嗪对成体大鼠脑缺血再灌注损伤后海马齿状回细胞增殖的影响 | |
| 引用本文： | 祁存芳,刘勇,田玉梅,张建水,陈新林,张蓬勃,邱芬,肖新莉,张军峰.川芎嗪对成体大鼠脑缺血再灌注损伤后海马齿状回细胞增殖的影响[J].西安交通大学学报(医学版),2007,28(2):142-144,160. |
| 作者姓名： | 祁存芳 刘勇 田玉梅 张建水 陈新林 张蓬勃 邱芬 肖新莉 张军峰 |
| 作者单位： | 1. 西安交通大学医学院人体解剖与组织胚胎学系神经科学研究中心,陕西西安,710061;青海医学院人体解剖学教研室,青海西宁,810001 2. 西安交通大学医学院人体解剖与组织胚胎学系神经科学研究中心,陕西西安,710061 3. 西安交通大学医学院人体解剖与组织胚胎学系神经科学研究中心,陕西西安,710061;西安交通大学医学院第二附属医院麻醉科,陕西西安,710004 |
| 摘 要： | 目的研究川芎嗪(tetramethylpyrazine,TMP)对大鼠脑缺血再灌注损伤后海马齿状回(dentate gyrus,DG)细胞增殖的影响。方法成年雄性SD大鼠行2 h大脑中动脉阻塞手术,术后2 h开始腹腔注射TMP40 mg/(kg.d)]。手术后腹腔注射5-溴脱氧尿核苷(5-bromodeoxyuridine,BrdU),末次注射24 h后处死动物,免疫组化染色观察TMP对脑缺血再灌注损伤后DG细胞增殖的作用。结果正常组和假手术组在DG有少量BrdU阳性细胞,对照组缺血后1 d阳性细胞开始增加,14 d达到高峰(P<0.05),TMP治疗组缺血后1 d损伤侧BrdU阳性细胞数开始增加,7 d达高峰(P<0.05)。结论TMP能促进缺血再灌注损伤大鼠海马齿状回内源性神经干细胞增殖。 |
| 关 键 词： | 脑缺血再灌注 海马齿状回 细胞增殖 川芎嗪 |
| 文章编号： | 1671-8259（2007）02-0142-03 |
| 修稿时间： | 2006-08-242006-11-28 |
Effects of tetramethylpyrazine on cell proliferation in dentate gyrus in rat brain after cerebral ischemia-reperfusion injury |
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| Qi Cunfang,Liu Yong,Tian Yumei,Zhang Jianshui,Chen Xinlin,Zhang Pengbo,Qiu Fen,Xiao Xinli,Zhang Junfeng.Effects of tetramethylpyrazine on cell proliferation in dentate gyrus in rat brain after cerebral ischemia-reperfusion injury[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2007,28(2):142-144,160. | |
| Authors: | Qi Cunfang Liu Yong Tian Yumei Zhang Jianshui Chen Xinlin Zhang Pengbo Qiu Fen Xiao Xinli Zhang Junfeng |
| Abstract: | Objective To observe the effects of tetramethylpyrazine(TMP) on cell proliferation in dentate gyrus(DG) in rat brain after cerebral ischemia-reperfusion injury.Methods Male SD rats were randomly divided into normal,sham-operation,control and TMP treatment groups.The control and TMP treatment groups were further divided into 5 subgroups: 1 d,3 d,7 d,14 d and 21 d reperfusion after 2 h middle cerebral artery occlusion(MCAO).The focal cerebral ischemia-reperfusion model was made by MCAO.S phase cells were labelled with BrdU.Immunohistochemistry method was conducted to detect the BrdU positive cells.The total number of BrdU positive cells in the DG was measured.Results There was a significant increase of BrdU positive cells in DG of TMP treatment on 3 d and 7 d groups compared to that in control group(P<0.05).The total number of BrdU positive cells reached a peak on d 7 and declined afterwards.Conclusion Tetramethylpyrazine may promote cell proliferation in DG in adult rats after ischemia-reperfusion injury. |
| Keywords: | cerebral ischemia-reperfusion dentate gyrus cell proliferation tetramethylpyrazine |
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