Cox-2、iNOS的表达及其与胃癌血管生成的关系

目的　探讨胃癌中环氧化酶2 (Cox 2)、诱导型一氧化氮合酶(iNOS)的表达及其与胃癌血管生成的关系。方法　用免疫组化SP法检测45例胃癌、癌旁组织手术切除标本Cox 2、iNOS和微血管密度(MVD)的表达。结果　胃癌组织Cox 2和iNOS的表达率以及MVD 均明显高于癌旁组织(77. 78% vs. 33. 33%, 68. 89% vs. 17. 78%,58.13±19.99vs. 24.02±10.28,P<0.01,P<0.005,P<0.05)。Cox 2 和iNOS均与MVD呈正相关(r=0.63,r=0.54,P<0.05,P<0.05)。Cox 2和iNOS表达有显著相关性(P<0.05)。结论　Cox 2 和iNOS在胃癌的发生中有协同作用,两者可能共同参与肿瘤血管的形成。

The relationship between the expression of Cox-2 and iNOS and angiogenesis in gastric carcinoma

Objective To evaluate the relationship between the expression of cyclooxygenase-2(Cox-2) and inducible nitric oxide synthase (iNOS) and the angiogenesis in gastric carcinoma. Methods Immunohistochemical stain was used to detect the expression of Cox-2, iNOS and MVD in 45 resected specimens of gastric carcinoma. The monoclonal antibody against CD34 was used for displaying vascular endothelial cells, and MVD was detected by counting the CD34-positive vascular endothelia cells. Paracancerous tissues were examined as the control. Results The expression rates of Cox-2, iNOS and MVD in gastric cancer were significantly increased, compared with those in the paracancerous tissues (77.78% vs. 33.33%, 68.89% vs. 17.78%, 58.13±19.99 vs. 24.02±10.28, P< 0.01 , P< 0.005 , P<0.05). Moreover, MVD in Cox-2 or iNOS-positive specimens was higher than that in Cox-2-or iNOS-negative specimens (61.29±14.31 vs. 45.38±12.42, 54.68±16.07 vs. 31.42±12.34, P< 0.05 , P< 0.001 ). Both Cox-2 and iNOS expression were positively correlated with MVD (r=0.63, r=0.54, P< 0.05 , P< 0.05 ). The expression rate of Cox-2 in the iNOS-positive specimens was significantly increased, compared with that of the negative ones (P< 0.05 ). Conclusion Cox-2 and iNOS might coordinatively cause the genesis of gastric carcinoma and be involved in tumorous angiogenesis in gastric carcinoma.