大骨节病和腺病毒感染的相关性研究

目的研究腺病毒感染与大骨节病(KBD)发病的关系。方法用巢式聚合酶链式反应(Nest-PCR)检测KBD病区和非病区儿童全血及KBD患者血清接种软骨细胞的腺病毒DNA,酶联免疫吸附实验(ELISA)检测KBD病区和非病区儿童血清腺病毒IgG抗体。结果KBD病区KBD儿童、病区对照组儿童和非病区儿童全血腺病毒DNA的阳性率分别为33.33%、34.92%和28.57%,腺病毒IgG抗体的阳性率分别为68.29%、65%和68.97%,差异无统计学意义(P>0.05);KBD患者血清培养细胞腺病毒DNA的PCR结果均为阴性。结论KBD病区和非病区儿童腺病毒均有较高的检出率;KBD的发病和腺病毒感染可能无直接病因学关系。

Relationship between Kashin-Beck disease and adenovirus infection

Objective In order to explore into the relationship between adenovirus(AdV)infection and Kashin-Beck disease(KBD).Methods Venous blood in children in KBD endemic area and non-endemic area were collected.Unknown virus was isolated using KBD patients' serum by chondrocytes culture.Adenovirus DNA in whole human blood and cells inoculated by KBD serum were detected by using nest polymerase chain reaction(Nest-PCR),adenovirus IgG antibody was detected by enzyme-linked immunosorbent assay(ELISA).Results Positive rate of adenovirus DNA in KBD children,healthy children in KBD endemic area and healthy children in non-endemic area was 33.33%,34.92% and 28.57%,respectively; positive rate of IgG antibody was 68.29%,65% and 68.97%,respectively.There was no significant difference between KBD patients and controls from endemic area and non-endemic area in both adenovirus DNA and IgG antibody positive rate(P>0.05);there was no positive sample when detecting adenovirus DNA of cell cultured isolated with KBD patients serum.Conclusion Although high infection rates of adenovirus were detected in Kashin-Beck endemic area and non-endemic area,there was no direct relationship between KBD and adenovirus infection.