胃癌患者血清NO、细胞因子和红细胞免疫功能的测定及其意义

目的　研究胃癌发病的免疫学机制。方法　应用形态学方法测定胃癌患者RBC C3bRR、RBC ICR及NTER ;用ELISA法测定血清TNF α、IL 6和IL 8含量 ;用生化方法测定血清NO含量。结果　胃癌患者血清NO含量 ,RBC C3bRR和NTER明显降低 (P均 <0 .0 5) ,术后较术前明显升高 (P均 <0 .0 5) ,但仍低于对照组 (P均 <0 .0 5)。血清TNF α、IL 6、IL 8含量及RBC ICR明显增高 (P均 <0 .0 5) ,术后血清IL 6、IL 8含量及RBC ICR较术前明显降低(P均 <0 .0 5) ,但仍高于对照组 (P均 <0 .0 5)。胃癌患者TNF α、IL 6、IL 8间呈正相关 ,RBC C3bRR与血清IL 6、IL 8含量呈负相关。结论　多项免疫指标的联合检测对揭示胃癌的发病机理、疗效观察和预后判断均有重要的临床意义

Detection and clinical significance of NO,cytokines in serum and red-cell immune functions on patients with gastric cancer

Objective To study the immune etiopathology in ga stric cancer. Methods The RBC-C 3b RR, RBC-ICR and NTER in patients with gastric cancer were detected by morphological methods, the contents of TNF-α ,IL-6 and IL-8 were detected by ELISA methods, and th e serum NO content was detected by biochemical methods. Results The serum NO content,RBC-C 3b RR and NTER in patients with gastric ca ncer were significantly lower ( P <0.05), and after removal of the carcinoma t he content of the serum NO, RBC-C 3b RR and NTER were significantly higher than that of the patients before operation ( P <0.05), but still lower than th at of controls ( P <0.05). The contents of the serum TNF- α , IL-6 and IL -8, RBC-ICR were significantly higher ( P < 0.05 ); after removal of the c arcinoma, the serum IL-6 and IL-8 contents,and RBC-ICR were significantly lo wer than those of the patients before operation ( P <0.05 ), but still higher than that of controls ( P <0.05). The serum TNF- α content had a positive correlation with serum IL-6 and IL-8 contents; the RBC-C 3b RR had a neg ative correlation with serum IL-6 and IL-8 contents of the patients. Conclusion The combination detections of the immune parameters have an important clinical significance in studying the etiopathogenesis, observation s on therapeutic efficacy and prognosis prediction.