| 绞股蓝总皂甙对P388细胞的作用及机制探讨 |
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| 引用本文： | 白元让,魏婉丽,茹祥斌,李凤仙.绞股蓝总皂甙对P388细胞的作用及机制探讨[J].西安交通大学学报(医学版),1997(3). |
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| 作者姓名： | 白元让 魏婉丽 茹祥斌 李凤仙 |
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| 作者单位： | 西安医科大学临床药理研究所!西安710061(白元让，魏婉丽)，军事医学科学院毒物药物研究所!北京100850(茹祥斌，李凤仙) |
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| 摘 要： | 用单层平皿软琼脂克隆法证明绞股蓝总皂甙对体外培养的P388细胞克隆生长有明显的抑制作用，其IC50为（22．5±1．0）mg·L-1。为进一步探讨其抑制P388细胞生长机理，用放射性同位素掺入法观察了绞股蓝总皂成对P388细胞DNA、RNA及蛋白质合成的影响。结果表明，在绞股蓝总皂甙作用24h内，其主要抑制P88细胞DNA合成，轻度抑制RNA合成，而对蛋白质合成几乎无影响。
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| 关 键 词： | 绞股蓝总皂甙 白血病 核酸 培养细胞 |
THE EFFECT OF GYPENOSIDES ON LEUKEMIA P388 CELLS AND ITS MECHANISM STUDY |
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| Bai Yuanrang, Wei Wanli, Ru Xiangbin et al.THE EFFECT OF GYPENOSIDES ON LEUKEMIA P388 CELLS AND ITS MECHANISM STUDY[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1997(3). |
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| Authors: | Bai Yuanrang Wei Wanli Ru Xiangbin |
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| Abstract: | Gypenosides (GP) inhibited cell growth of leukemia P388 in vitro by using cellular colony-forming assay method. The 50% inhibitory concentration of cell growth was (22. 5±1. 0)mg'L-1 respectively. In order to studv its inhibitory mechanism, radioactive isotope was incorporated in GP-supplemented 24 hours P388 cells. The results showed that GP mainly decreased DNA synthesis of P388 cells, slightly inhibited RNA synthesis and al-most had no effect on protein synthesis. |
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| Keywords: | gypenosides leukemia ncleic acid culture cell |
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