| 兔骨髓基质干细胞的体外培养及生物学活性研究 |
| |
| 引用本文： | 张晨,王坤正,强辉,时志斌,党晓谦,黄向辉.兔骨髓基质干细胞的体外培养及生物学活性研究[J].西安交通大学学报(医学版),2010,31(2). |
| |
| 作者姓名： | 张晨 王坤正 强辉 时志斌 党晓谦 黄向辉 |
| |
| 作者单位： | 1. 西安交通大学医学院第二附属医院骨科,陕西西安,710004
2. 陕西省人民医院骨科,陕西西安,710068 |
| |
| 基金项目： | 国家自然科学基金资助项目(No.30600624)Supported by the National Natural Science Foundation of China |
| |
| 摘 要： | 目的 体外分离培养、鉴定兔骨髓基质干细胞(BMSCs)并探讨其分化潜能.方法 应用密度梯度离心联合贴壁筛选的方法,体外分离培养兔骨髓来源的骨髓基质干细胞,培养过程中倒置显微镜下观察其生物学特性,HE染色和CD34、CD44免疫组织化学染色行细胞学鉴定;应用体外成骨与成脂诱导分化检测其生物学活性.结果 体外实验成功分离培养兔BMSCs,HE染色显示细胞均一性好;CD44强阳性表达,CD34阴性表达.诱导成骨结果显示BMSCs具有较强的成骨活性,ALP染色阳性;成脂诱导结果显示BMSCs具有较强的成脂活性,油红"O"脂肪染色阳性.结论 BMSCs体外具有潜在的多向分化能力.
|
| 关 键 词： | 骨髓基质干细胞 细胞培养 多向分化 |
Biological activity of the cultured rabbit bone marrow-derived mesenchymal stem cells |
| |
| ZHANG Chen,WANG Kun-zheng,QIANG Hui,SHI Zhi-bin,DANG Xiao-qian,HUANG Xiang-hui.Biological activity of the cultured rabbit bone marrow-derived mesenchymal stem cells[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(2). |
| |
| Authors: | ZHANG Chen WANG Kun-zheng QIANG Hui SHI Zhi-bin DANG Xiao-qian HUANG Xiang-hui |
| |
| Institution: | in vitroZHANG Chen1,WANG Kun-zheng1,QIANG Hui1,SHI Zhi-bin1,DANG Xiao-qian1,HUANG Xiang-hui2(1.Department of Orthopedics,the Second Affiliated Hospital,Medical School ofXi\'an Jiaotong University,Xi\'an 710004,2.Department of Orthopedics,Shaanxi Provincial People\'s Hospital,Xi\'an 710068,China) |
| |
| Abstract: | Objective To observe the effect of the in vitro isolation,culture and identification of rabbit bone marrow-derived mesenchymal stem cells(BMSCs) and explore the differentiation potentials of BMSCs.Methods BMSCs were isolated and purified by density gradient centrifugation combined with attachment culture method.The biological characteristics of BMSCs were observed under an inverted microscope.The BMSCs were identified with HE staining and CD34/CD44 immunohistochemical staining.The biological activity of BMS... |
| |
| Keywords: | bone marrow-derived mesenchymal stem cell cell culture multi-directional differentiation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
