腹腔巨噬细胞在大鼠重症急性胰腺炎发病机制中的作用

目的探讨腹腔巨噬细胞(PMA)在大鼠重症急性胰腺炎(SAP)进展中的作用。方法SD大鼠随机分为假手术组、SAP组。通过逆行胰管注射40 g/L牛黄胆酸钠(0.1 mL/100 g)建立大鼠SAP模型。分别于术后3、6、12 h剖杀大鼠。分离PMA并培养24 h。RT-PCR法检测PMA中肿瘤坏死因子-α(TNF-α)mRNA和白介素-1β(IL-1β)mRNA的表达;酶联免疫吸附法检测细胞培养液及血清中TNF-α和IL-1β的水平。取胰腺组织行HE染色及病理学评分。结果SAP组PMA中TNF-αmRNA和IL-1βmRNA的表达、细胞培养液及血清中TNF-α和IL-1β水平均高于假手术组(P<0.01)。病理学评分证实SAP组胰腺组织损伤程度随时间延长而迅速加重(P<0.01)。结论PMA可以通过分泌大量的TNF-α和IL-1β等细胞因子影响SAP的严重程度。

The role of peritoneal macrophages in the pathogenesis of severe acute pancreatitis in rats

Objective To investigate the role of peritoneal macrophages(PMA) in the pathogenesis of severe acute pancreatitis(SAP) in rats.Methods Sprague-Dawley(SD) rats were randomly divided into control group and SAP group.To induce SAP in the rats,40g/L sodium taurocholate(0.1mL/100g) was injected to pancreatic duct through retrograde exposure of pancreatic bile duct in hepatic porta.One-third rats were sacrificed 3,6 or 12h after the injection.PMA were extracted,and incubated for 24h in a humidified 5% carbon dioxide incubator.The expressions of tumor necrosis factor alpha(TNF-α) mRNA and interleukin-1β(IL-1β) mRNA in PMA were measured by semi-quantitative RT-PCR.The levels of TNF-α and IL-1β in culture medium and serum were evaluated.The histological changes in pancreas were examined.Results The expressions of TNF-α mRNA and IL-1β mRNA in PMA were significantly higher in SAP group than those in control group at each time point((P<0.01)).The concentrations of TNF-α and IL-1β in culture medium and serum were significantly increased in SAP group when compared with those in control group(P<0.01).The histological analysis of pancreas indicated that the damage in SAP group was more serious than that in control group(P<0.01).Conclusion PMA can affect the severity of SAP through secreting a large number of cytokines like TNF-α and IL-1β.