干扰素α_(2b)抑制大鼠肝纤维化形成的实验研究

以 40 %四氯化碳花生油诱导大鼠肝纤维化模型 ,同时用重组干扰素 α2 b( IFN-α2 b)及秋水仙碱 ( COL )做预防性治疗 ,观察 IFN-α2 b对大鼠肝纤维化形成的影响。结果发现 IFN- α2 b及 COL治疗组血清 AL T、AST、 型前胶原、 型胶原及透明质酸水平 ,以及肝组织纤维化程度均显著低于模型组 ,IFN- α2 b组肝组织炎症活动度及 、 型胶原 ,TNF- α显色指数均显著小于模型组。作者认为 ,IFN- α2 b可通过保护肝细胞、减轻肝组织炎症坏死、抑制 TNF- α对贮脂细胞的激活等机制 ,而抑制大鼠肝纤维化的形成

THE EXPERIMENTAI STUDY OF INTERFERON α_(2b) INTERFERING HEPATIC FIBROTIC FORMATION IN RATS

The aim of the study is to evaluate the effect of interferon α 2b (IFN α 2b ) on hepatic fibrotic formation .We used IFN α 2b and colchicine on murine hepatic fibrosis model induced by carbon tetrachloride.The results showed:① The levels of serum alanine aminotransferase, aspartate aminotransferase, procollagen type Ⅲ,type Ⅳ collagen and hyaluronic acid in IFN α 2b and colchicine groups were significantly lower than those of the model group, and the degree of hepatic fibrosis were also lessening markedly in treated groups;②The degrees of inflammatory activity and coloration index of type I collagen, type Ⅲ collagen and tumor necrosis factor α in IFN α 2b group were significantly decreased than those of the model group.It implies that IFN α 2b has good interfering effect on hepatic fibrotic formation in rats,and the mechanism lie in protecting liver cell,inhibiting the inflammation of hepatic tissue, restraining the activity of fat storing cell induced by tumor necrosis factor α.