| 钙拮抗剂尼群地平的反相高效液相色谱分析及其在家兔体内的分布 |
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| 引用本文： | 傅强,贺浪冲,扬广德,常春,武玥.钙拮抗剂尼群地平的反相高效液相色谱分析及其在家兔体内的分布[J].西安交通大学学报(医学版),1998(2). |
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| 作者姓名： | 傅强 贺浪冲 扬广德 常春 武玥 |
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| 作者单位： | 西安药学院药物分析教研室!西安，710061，西安药学院药物分析教研室!西安，710061，西安药学院药物分析教研室!西安，710061，西安药学院药物分析教研室!西安，710061，西安药学院药物分析教研室!西安，710061 |
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| 摘 要： | 对兔血浆及组织器官中钙拮抗剂尼群地平的分析方法进行了研究，建立了反相高效液相色谱（RP-HPLC）的内标测定法。样品在弱碱性条件下经环已烷-异丙醇（96．5：3．5）提取，用YWGC18反相柱，以地尔硫作内标，甲醇：50mmol／L磷酸盐缓冲液（70：30）pH6．8为流动相，UV236nm检测。方法最低检测限约为10ng，血药浓度在21．6～216μg／L之间，尼群地平的量与其峰面积比值之间，线性关系良好，回归方程为Y＝0．00672X－0．013，r＝0．9958。方法回收率95．4％～98．1％，RSD＜4．63％。 用该分析方法测定了家兔口服尼群地平2h后在主要脏器体液中的分布。
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| 关 键 词： | 尼群地平 钙拮抗剂 高效液相色谱 分布 |
RP-HPLC ANALYSIS OF NITRENDIPINE AND ITS DISTRIBUTION STUDY IN RABBITS |
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| Fu Qiang, He Langchong, Yang Guangde et al.RP-HPLC ANALYSIS OF NITRENDIPINE AND ITS DISTRIBUTION STUDY IN RABBITS[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1998(2). |
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| Authors: | Fu Qiang He Langchong Yang Guangde |
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| Abstract: | A analytical method for determination of nitrendipine, a calcium antagonist, in serum,tissues and organs of rabbits was developed. Inweak alkaline conditions, samples were extractedwith cyclohexane-isopropanol (96. 5: 3. 5. v/v ) and analyzed with YWG C18 reversed phase column at uv236um. Diltiazem was used as internal standard and methanol: 50mmol/L phosphate buffer(70: 30v/v)was used as mobile phase. The minimum detectionlimit of nitrendipine was 10ng. The liner range wasbetween 21. 6 ～ 216ng/ml with regression equationY = 0.00673X - 0.013. r = 0. 9958. The analyticalrecoveries were 95. 4% ～98. 1% with RSD less than4. 63 %. This analytical procedure was employed todetermin nitrendipine distribution in rabbits 2 hoursafter the drug was administered. |
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| Keywords: | nitrendipine calcium antogonists high-performunce liquid chromatography distribution |
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