单克隆抗体在急慢性白血病分型中的应用

本文应用国风产一系列抗非淋巴细胞单克隆抗体(单抗),以免疫荧光技术,对45例急性非淋巴细胞白血病(ANLL)和36例慢性粒细胞白血病(CML)的细胞表面抗原进行了检测与分型,比较了ANLL免疫学分型与细胞形态学分型的关系和CML急变期与慢性期抗原的不同点;并对4例形态上难以分型的急性白血病(AL)进行了免疫学分型。结果发现:国产的一系列抗非淋巴细胞单抗,可把FAB_(M1～M5)分为原粒、早幼粒及幼单单核细胞型;ANLL免疫学分型与形态学分型有关,但又不完全一致;28例慢性期CML全部与一个中性粒细胞的单抗反应,而8例急变期CML原始细胞无此抗原表达。4例细胞形态学分型困难的AL经单抗进行免疫学分型,3例为急淋,1例为急非淋。

INITIAL USE OF MONOCLONAL ANTIBODIES (McAbs) FOR THE TYPING OF ACUTE NON-LyMPHOID I.EUKEMIA(ANLL) AND CHRONIC MyELOCyTIC LEUKEMIA(CML)

The leukemic cellular surface antigens of 45 cases for ANLL and 36 cases of CML were detected and classified with a series of the domestic McAbs by immunoflurescent technique. The immunophenotype of ANLL was compared with its cellular morphologic classification. The difference of the surface antigens between the CML instable phase and the CML in blast crisis was analyzed. Four cases of acute leukemias (AL) which could not be classified morphologically were classified by McAbs. FAB M_1～M_5 could be divided into 3 phenotype groups: myeloblastic, promylocytic and promoncytic phenotype. The immunophenotype of ANLL tended to agree with its cellular morphologic classification. All 28 cases of CML in stable phase reacted with a neutrophil-McAb. However, there was no this antigen expression in 8 cases of CML in blast crisis. The immunophenotype of 4 cases of AL could be made with McAbs . Of these 4 cases. 3 were AL and 1 ANLL.