用改良的荧光探针法测定外周血淋巴细胞膜流动性及其临床应用

用改良的荧光探针法测定外周血淋巴细胞膜流动性，此方法不需分离细胞膜，操作简便，灵敏度高，批内变异系数CV为0．9％。采用该方法对41例心力衰竭患者及20例正常人外周淋巴细胞膜流动性进行了测定，结果显示：心力衰竭患者外周淋巴细胞膜流动性P值为0．345±0．021，正常人为0．259±0．019，二者有显著差异（P＜0．05）；且发现外周淋巴细胞膜流动性改变与红细胞的改变一致，相关分析表明二者呈正相关（r=0．937，P＜0．05）。

USING IMPROVED FLUORESCENCE PROBE ASSAYING LYMPHOCYTE MEMBRANE FLUIDITY AND ITS CLINICAL USES

In this paper, the lymphocyte membrane fluidity was abserved using improved fluorescence probe. This method did not need to seperatelymphocyte membrane, and it is simple and sensitive. The inter-assay CV was 0. 9%. Using thismethod, the lymphocyte membrane fluidity wasstudied in 20 health people and 41 patients withheart failure. The results showed that the lymphocvte membrane fluorescence polarization is 0. 259±0. 019 in health group and 0. 345±0. 021 in patientgroup. The latter increased significantly (P<0. 05). The change of lymphocyte membrane fluiditywas the same as that of seperated erythrocyte membrane. There was positive correlation between thelymphocyte membrane fluidity and seperated erythrocyte membrane fluidity (r=0. 937, P<0. 05).