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| 编码表皮生长因子受体Ⅲ型突变体PEP-3表位的cDNA的克隆 | |
| 引用本文： | 段小艺,王健生,郭佑民,刘敏,周斌,王全颖,杨广笑.编码表皮生长因子受体Ⅲ型突变体PEP-3表位的cDNA的克隆[J].西安交通大学学报(医学版),2006,27(2):140-142. |
| 作者姓名： | 段小艺 王健生 郭佑民 刘敏 周斌 王全颖 杨广笑 |
| 作者单位： | 1. 西安交通大学医学院第一附属医院核医学科,陕西,西安,710061 2. 西安交通大学医学院第一附属医院肿瘤外科,陕西,西安,710061 3. 西安交通大学医学院第二附属医院影像中心,陕西,西安,710004 4. 西安华广生物工程公司,陕西,西安,710025 |
| 摘 要： | 目的克隆出针对表皮生长因子受体Ⅲ型突变体(EGFRvⅢ)PEP-3表位的cDNA片段,为高表达EGFRvⅢ肿瘤的免疫治疗奠定基础。方法根据PEP-3的碱基序列设计出有12对互补碱基的正负引物,正负引物互为模板进行PCR扩增,制备出两端含酶切位点的PEP-3 cDNA片段,再将扩增的PCR产物亚克隆于载体pGEM-T Easy构建重组质粒pGEM-T Easy/PEP-3。结果经限制性内切酶酶切鉴定和DNA测序证实,编码PEP-3的cDNA片段被成功扩增并亚克隆于载体pGEM-T Easy中。结论成功克隆了编码表皮生长因子受体Ⅲ型突变体PEP-3表位的cDNA片段。 |
| 关 键 词： | 表皮生长因子受体Ⅲ型突变体 PEP-3表位 cDNA 基因克隆 |
| 文章编号： | 1671-8259(2006)02-0140-03 |
| 收稿时间： | 2005-10-24 |
| 修稿时间： | 2005-12-10 |
Clone of cDNA encoding PEP-3 epitope of epidermal growth factor receptor type Ⅲ mutant |
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| Duan Xiaoyi,Wang Jiansheng,Guo Youmin,Liu Min,Zhou Bin,Wang Quanying,Yang Guangxiao.Clone of cDNA encoding PEP-3 epitope of epidermal growth factor receptor type Ⅲ mutant[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2006,27(2):140-142. | |
| Authors: | Duan Xiaoyi Wang Jiansheng Guo Youmin Liu Min Zhou Bin Wang Quanying Yang Guangxiao |
| Abstract: | Objective Through cloning the cDNA fragment encoding PEP-3 epitope of epidermal growth factor receptor type Ⅲ mutant(EGFRvⅢ) to establish the base for immunotherapy of carcinoma with high EGFRvⅢ expressing.Methods Designing the primer according to the base sequence of PEP-3,cDNA was synthesized and amplificated by PCR.Then,the PCR product was subcloned into vector pGEM-T Easy so as to construct recombinant plasmid pGEM-T Easy/ PEP-3.Results Zymo-cutting restriction enzyme identification and DNA sequencing conformed that cDNA encoding PEP-3 was amplified and subcoloned into pGEM-T Easy successfully.Conclusion cDNA encoding PEP-3 epitope of EGFRvⅢ was coloned successfully. |
| Keywords: | cDNA |
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