| pH梯度-冻融法制备硝苯地平脂质体 |
| |
| 引用本文： | 李维凤,牛晓峰,陈海英,赵桂兰.pH梯度-冻融法制备硝苯地平脂质体[J].西安交通大学学报(医学版),2003,24(6):578-580. |
| |
| 作者姓名： | 李维凤 牛晓峰 陈海英 赵桂兰 |
| |
| 作者单位： | 1. 西安交通大学药学院药剂学教研室,陕西西安,710061
2. 西安交通大学药学院药学院2002届毕业生,陕西西安,710061 |
| |
| 基金项目： | 陕西省自然科学基金资助项目 (No .2 0 0 1SM 72 ) |
| |
| 摘 要： | 目的 优选硝苯地平脂质体的制备工艺 ,并评价其质量。方法 采用pH梯度 冻融法制备硝苯地平脂质体 ,以包封率和稳定性为检测指标。用SephadexG 5 0微型柱分离 紫外分光光度法测包封率 ;并比较了冻融前后的包封率、粒度分布 ;考察了冻融次数对包封率的影响。结果 本实验制备的脂质体包封率达到 96 .6 5 % ,冻融前的平均粒度为0 .0 98μm ,冻融后的平均粒度为 2 .0 μm。经冻融处理后 ,脂质体包封率和稳定性明显提高。结论 优选得到的脂质体制备工艺稳定可行
|
| 关 键 词： | 硝苯地平 脂质体 稳定性 |
| 文章编号： | 1671-8259(2003)06-0578-03 |
| 修稿时间： | 2003年1月20日 |
Preparation of nifedipine liposomes by pH steps degree-freeze thawing method |
| |
| Li Weifeng,Niu Xiaofeng,Chen Haiying,Zhao Guilan.Preparation of nifedipine liposomes by pH steps degree-freeze thawing method[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2003,24(6):578-580. |
| |
| Authors: | Li Weifeng Niu Xiaofeng Chen Haiying Zhao Guilan |
| |
| Abstract: | Objective To study the preparation of nifedipine liposomes (NP) and evaluate their quality. Methods The liposomes were prepared by a pH steps degree-freeze thawing method, with the entrapment and stability as indices. The entrapment was studied by Sephadex G-50 minicolumn centrifugation UV-Vis spectroscopy method. Entrapment, and degree distribution before and after freezing were compared, the effect of freezing times on entrapment was studied. Results The entrapment and the average size of NP liposomes were 96.65% and 2.0 μm, respectively. After freezing, both the entrapment and stability were increased. Conclusion The selected formulation and preparation of nifedipine liposomes are practicable. |
| |
| Keywords: | nifedipine liposome stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
