胎盘组织中Human Annexin V的表达及与HBV感染的关系

目的观察Human AnnexinV(HAV)在胎盘组织中的表达,并探讨其在乙型肝炎病毒(HBV)感染胎盘中的可能作用。方法用免疫荧光染色法检测早孕绒毛,中孕和足月胎盘绒毛中HAV的表达;用免疫荧光双重染色法观察HBV感染的胎盘组织HBsAg和HAV的分布及相互关系。结果①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。②HBV感染的胎盘绒毛中只要是HBsAg阳性的部位同时一定有HAV的表达,阳性信号存在于滋养层细胞、绒毛间质细胞、绒毛毛细血管内皮细胞中。结论HAV可能作为HBsAg的特异性受体介导了HBV与胎盘组织各层细胞的粘附、内化等过程并最终导致了HBV对胎盘的感染。

Expression of Human Annexin V in placenta and its effects on placenta infected by HBV

Objective To investigate the expression of Human Annexin V(HAV) in placenta and to explore the possible role of it in HBV infected placenta.Methods HAV was detected in placentas that had been proved infected with HBV with immunofluorescence assays and the double-label immunofluorescence assays were used to detect HBsAg and HAV in the same placenta.Confocal laser scanning microscope was also used to observe the result.Results ① HAV was expressed on early period,metaphase and term villi.It was expressed on trophoblastic cell,interstitial cell and endothelium cell of interstitial blood vessels of villi.The result had no relation with the infection of HBV and the time of pregnancy.② The double-label immunofluorescence assays showed the positive area of HBsAg in placentas accorded with the area of HAV.Conclusion HAV mediated HBV into cells of placenta as specific receptor and resulted in infection.