内皮素-1和干细胞因子对黑素细胞黏附和迁移影响的对照研究

目的　体外研究内皮素 1(endothelin 1,ET 1)和干细胞因子 (stemcellfactor,SCF)对黑素细胞黏附和迁移的影响。方法　分别用ET 1和SCF处理人表皮纯化培养的黑素细胞 ,观察其在纤维连接素包裹的培养板上黏附以及通过微孔滤膜的情况 ,并作对照比较。结果　与SCF处理相比 ,ET 1处理的黑素细胞易黏附于培养板和通过微孔滤膜 ,尤其是当其浓度在 32nmol·L-1时 ;当浓度≥ 12 8nmol·L-1时 ,对黑素细胞的黏附和迁移却呈现抑制作用 (P <0 .0 1)。通过对黑素细胞的迁移观察 ,发现ET 1的浓度≥ 2nmol·L-1时 ,均对黑素细胞迁移呈促进作用 ,这种作用强于SCF处理的黑素细胞 (P <0 .0 1) ,并随其浓度增加而增强。结论　ET 1可以促进黑素细胞的黏附和迁移 ,其效果优于SCF。

The effects of endothelin-1 and stem cell factor on melanocyte adhesion and migration in vitro

Objective To study the effects of endothelin-1 ( ET-1) and stem cell factor (SCF) on melanocyte adhesion and migration in vitr o. Methods Human epidermal melanocytes that had been cultured and purified were treated with ET-1 and observed for adhesion to bovine serum f ibronectin-coated culture dishes. SCF and ET-1 treated cells were also examine d for migration into micropore filters coated with the same protein. Results Compared with SCF group, ET-1 treated melanocytes adhered to the dishes and moved into the filters more easily, especially when th e concentration was at 32 nmol·L -1. When the concentration of ET-1 was 128 mol·L -1 or more, melanocyte adhesion and migration were inhibited ( P< 0.01); when the concentration of ET-1 was at 2 nmol·L -1 or mo re, migration increased obviously compared with SCF treated cells (P<0.01). Conclusion ET-1 is more effective in enhancing melanocyte adhesion and migration than SCF.