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| 骨髓间充质干细胞对5/6肾切除大鼠肾脏损伤时Bcl-2、Bax表达的影响 | |
| 引用本文： | 袁浩峥,姚钢炼,付荣国,刘晓丹,张莉,赵丽燕,李向利,薛荣亮.骨髓间充质干细胞对5/6肾切除大鼠肾脏损伤时Bcl-2、Bax表达的影响[J].西安交通大学学报(医学版),2011,32(5):585-588. |
| 作者姓名： | 袁浩峥 姚钢炼 付荣国 刘晓丹 张莉 赵丽燕 李向利 薛荣亮 |
| 作者单位： | 1. 西安交通大学医学院第二附属医院麻醉手术科 2. 西安交通大学医学院第二附属医院肾病科,陕西西安,710004 3. 西安市第四人民医院肾病科,陕西西安,710004 |
| 基金项目： | 国家自然科学基金，西安交通大学台湾光华基金 |
| 摘 要： | 目的探讨骨髓间充质干细胞(BMSCs)移植对5/6肾切除大鼠肾损伤的保护作用,观察BMSCs对肾组织Bcl-2、Bax表达的影响。方法将雄性SD大鼠BMSCs用PKH26标记后注入受体雌性SD大鼠体内。40只受体雌性SD大鼠随机分为4组(n=10):假手术组、5/6肾切除+DMEM-F12组(模型组)、5/6肾切除+贝那普利组(对照组)、5/6肾切除+BMSCs组(干细胞组)。60d后观察肾功能改变;免疫组化法检测Bcl-2和Bax的表达,并观察PKH26标记的BMSCs在受体大鼠肾脏的分布情况。结果假手术组肾功能明显好于其余各组(P<0.05),对照组和干细胞组肾功能明显好于模型组(P<0.05);模型组Bcl-2、Bax表达及Bcl-2/Bax比值明显高于其余各组(P<0.05),干细胞组Bcl-2和Bax表达明显高于对照组(P<0.05)。5/6肾切除后第60d可见标记的BMSCs定位于肾组织中。结论外源性BMSCs可减少Bcl-2和Bax蛋白表达,下调Bcl-2/Bax比值,减少肾组织细胞凋亡,从而有利于肾功能的早期恢复。 |
| 关 键 词： | 慢性肾损伤 骨髓间充质干细胞 细胞凋亡 Bcl-2 Bax |
Effects of bone mesenchymal stem cells on Bcl-2 and Bax expressions in the progression of renal injury in 5/6 nephrectomy rats |
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| YUAN Hao-zheng,YAO Gang-lian,FU Rong-guo,LIU Xiao-dan,ZHANG Li,ZHAO Li-yan,LI Xiang-li,XUE Rong-liang.Effects of bone mesenchymal stem cells on Bcl-2 and Bax expressions in the progression of renal injury in 5/6 nephrectomy rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(5):585-588. | |
| Authors: | YUAN Hao-zheng YAO Gang-lian FU Rong-guo LIU Xiao-dan ZHANG Li ZHAO Li-yan LI Xiang-li XUE Rong-liang |
| Institution: | YUAN Hao-zheng1,YAO Gang-lian2,FU Rong-guo2,LIU Xiao-dan2,ZHANG Li3,ZHAO Li-yan1,LI Xiang-li1,XUE Rong-liang1(1.Department of Anesthesiology,the Second Affiliated Hospital,Medical School ofXi'an Jiaotong University,Xi'an 710004,2.Department of Nephrology,Medical School of Xi'an Jiaotong University,3.Department of Nephrology,the Fourth Hospital of Xi'an,China) |
| Abstract: | Objective To study the protective effects of bone marrow mesenchymal stem cells(BMSCs) on 5/6 nephrectomy rats as well as observe the expressions of Bcl-2 and Bax proteins in the progression of renal injury.Methods BMSCs were isolated from male SD rats and labeled with PKH26 before injection.Totally 40 female recipient SD rats were divided randomly into four groups: sham group,DMEM-F12 infusion group(model group),benazepril gastric instillation group(control group) and BMSCs injection group(BMSCs group),eac... |
| Keywords: | chronic renal injury bone mesenchymal stem cell cell apoptosis Bcl-2 Bax |
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