| 参芎化瘀胶囊通过PI3-K/Akt信号通路改善大鼠缺血性脑损伤 |
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| 引用本文： | 赵雅宁,李建民,张姗姗,陈长香,马素慧.参芎化瘀胶囊通过PI3-K/Akt信号通路改善大鼠缺血性脑损伤[J].西安交通大学学报(医学版),2011,32(5):636-639. |
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| 作者姓名： | 赵雅宁 李建民 张姗姗 陈长香 马素慧 |
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| 作者单位： | 河北联合大学康复医学院,河北唐山,063000 |
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| 摘 要： | 目的探讨参芎化瘀胶囊对缺血性脑损伤的治疗作用及其机制。方法 SD大鼠分为假手术组、模型组及参芎化瘀胶囊高、低剂量组。改良的Pulsineli 4血管阻断(4-VO)法制作全脑缺血模型;HE染色观察海马区神经细胞的形态变化;免疫组织化学法检测海马区磷脂酰肌醇3-激酶(PI3-K)、蛋白激酶B(PKB/Akt)的表达;原位缺口末端标记法(TUNEL)检测凋亡细胞;八臂迷宫法测试动物学习记忆功能。结果与假手术组比较,模型组大鼠海马区神经元结构损伤明显,PI3-K、Akt表达增高,凋亡神经细胞数量增加,大鼠的学习记忆功能下降;与模型组比较,参芎化瘀胶囊组海马神经元形态结构损伤减轻,PI3-K、Akt表达进一步增多,凋亡神经细胞数量减少,动物学习记忆功能改善,上述变化在高剂量参芎化瘀胶囊更为明显。结论参芎化瘀胶囊对脑缺血损伤有很好的治疗作用,其机制可能与调控PI3-K/Akt信号途径有关。
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| 关 键 词： | 脑缺血 细胞凋亡 磷脂酰肌醇3-激酶 蛋白激酶B 参芎化瘀胶囊 |
Shenxionghuayu capsule attenuates cerebral ischemic injury by regulating PI3-K/Akt signal pathway in rats |
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| ZHAO Ya-ning,LI Jian-min,ZHANG Shan-shan,CHEN Chang-xiang,MA Su-hui.Shenxionghuayu capsule attenuates cerebral ischemic injury by regulating PI3-K/Akt signal pathway in rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(5):636-639. |
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| Authors: | ZHAO Ya-ning LI Jian-min ZHANG Shan-shan CHEN Chang-xiang MA Su-hui |
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| Institution: | ZHAO Ya-ning,LI Jian-min,ZHANG Shan-shan,CHEN Chang-xiang,MA Su-hui(Department of Rehabilitation Medicine,Hebei United University,Tangshan 063000,China) |
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| Abstract: | Objective To investigate the effects of Shenxionghuayu capsule on cerebral ischemia and its underlying mechanism in rats.Methods Male Sprague-Dawley rats were randomly divided into four groups: sham-operation group,model group,and low-dose and high-dose Shenxionghuayu capsule treatment groups.Global cerebral ischemia model was constructed by modified four-vessel occlusion according to Pulsinelli's method.Morphological changes in the hippocampal region were observed by HE staining.The expression of phosphati... |
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| Keywords: | cerebral ischemia apoptosis phosphatidylinositol 3-kinase(PI3-K) serine/threonine kinase(Akt) Shenxionghuayu capsule |
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