各种实验因素对建立大鼠吗啡依赖模型的影响

目的　评价在建立大鼠吗啡依赖模型中 ,各种实验因素对吗啡依赖形成的影响。方法　将 130只大鼠随机分为正常组和依赖组 ,比较在不同剂量、不同时间及恒量法、递增法给药时 ,正常组和依赖组的戒断症状评分。结果　在DG1、DG2 、DG3 不同剂量组中 ,DG2 、DG3 剂量组的正常组与依赖组之间均有显著性差异 (P <0 .0 5 ) ,在依赖组内 ,DG2 、DG3 剂量组与DG1组之间也有显著性差异 (P <0 .0 5 ) ;在 3、5、7d不同给药时间组中 ,7d组的正常组与依赖组之间有显著性差异 (P <0 .0 5 ) ,在依赖组内 ,7d组和 3、5d组相比较有显著性差异 (P <0 .0 5 ) ;在恒量组和递增组中 ,正常组和依赖组之间均有显著性差异 (P <0 .0 5 ) ,在依赖组中 ,剂量恒定组与递增组之间相比较也有显著性差异 (P <0 .0 5 )。结论　不同给药剂量、不同给药时间、恒量给药和递增给药对吗啡依赖的形成均有明显的影响。因此 ,在进行吗啡依赖机制的研究中要重视这些影响因素 ,选择最佳实验方法。

Influence of several experimental factors on morphine dependence model in rats

Objective To study the influenc e of several experimental factors on morphine dependence in rats. Methods The 130 SD health rats were randomly assigned to control groups and dependence groups. The withdrawal syndromes between control groups and dependen ce groups in different dosage, different time, constant dose administration and increased dose administration were evaluated. Results The differences between control groups and dependen ce groups were significant (P<0.05) in DG 2 and DG 3, and the diff erences between DG 2, DG 3 and DG 1 were also significant (P< 0.05) in the same dependence groups. In 3 d, 5 d and 7 d groups, the differen ces between control groups and dependence groups were significant (P<0.05) i n 7 d, and the differences between 3 d, 5 d and 7 d were significant (P< 0.05) in the same dependence groups. The differences between control groups and dependence groups were all significant (P<0.05) in constant groups and incre ased groups, and the differences between constant groups and increased groups we re also significant (P<0.05) within the same dependence groups. Conclusion Morphine dependence forming process is related t o several experimental factors, including different dosage, different time, cons tant dose, and increased dose. Therefore, more attention should be paid to these experimental factors in setting up morphine dependence model and choosing the b est experimental methods.