| 经直肠豚鼠志贺菌痢疾模型的改良 |
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| 引用本文： | 张晨华,许丹,王一理.经直肠豚鼠志贺菌痢疾模型的改良[J].西安交通大学学报(医学版),2011,32(2):258-260. |
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| 作者姓名： | 张晨华 许丹 王一理 |
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| 作者单位： | 西安交通大学生命科学与技术学院生物工程系疫苗研发中心,陕西西安,710061 |
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| 摘 要： | 目的改良经直肠途径建立豚鼠志贺菌痢疾动物模型的方法。方法 20只受试豚鼠根据感染前24 h禁食或不禁食处理分为2组,8只非禁食组参照文献经直肠灌注致病性福氏志贺菌sf301菌株,12只禁食组经直肠分别灌注PBS(5只)和sf301菌株(7只),观察受试豚鼠的感染症状,通过组织病理学方法证实其发病与否。结果非禁食组豚鼠仅有1只(1/8)在感染后24 h内表现出明显的体重减轻、发热等典型的菌痢症状。禁食组经直肠灌注sf301菌株的豚鼠(6/7)在感染后24 h内有显著的发热症状,体重减轻则发生在感染后的24~48 h内;灌注PBS的豚鼠(0/5)无异样症状。组织病理学结果显示,发病豚鼠结肠末端的黏膜以及黏膜下层遭到明显的破坏,肠壁增厚,出现水肿、糜烂、大量中性粒细胞浸润等。结论成功建立了改良的经直肠豚鼠志贺菌痢疾动物模型。
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| 关 键 词： | 志贺氏菌 细菌性痢疾 疫苗 动物模型 |
The improvement of shigellosis model in guinea pigs through the intra-rectal route |
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| ZHANG Chen-hua,XU Dan,WANG Yi-li.The improvement of shigellosis model in guinea pigs through the intra-rectal route[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(2):258-260. |
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| Authors: | ZHANG Chen-hua XU Dan WANG Yi-li |
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| Institution: | ZHANG Chen-hua,XU Dan,WANG Yi-li(Center for Vaccine Development,Department of Biological Engineering,School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective To improve an animal model of shigellosis in guinea pigs through the intra-rectal route.Methods Twenty guinea pigs were divided into two groups according to the strategy of pretreatment with starvation for 24h or without before infection.Eight non-starvation guinea pigs were administered with pathogenic Shigella flexneri strain(sf301) by the intra-rectal(i.r.) route exactly following the literature.Those in the starvation group were inoculated with PBS(5 guinea pigs) as controls and with strain sf... |
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| Keywords: | Shigella bacillary dysentery vaccine animal model |
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