胃肠癌的MG_7抗原表达及其免疫生物学

应用免疫组化技术对62例胃肠癌的MG_7,抗原表达和局部免疫应答状态进行了分析。结果发现:MG_7抗原的检出率在胃癌为80.77%(21/26例),结直肠癌为94.44%(34/36例),淋巴结转移癌为93.33%(14/15例)。除2例癌旁非典型增生粘膜上皮呈弱阳性外,其余正常。良性病变粘膜上皮均为阴性。而且MG_7抗原表达强度与肿瘤恶性程度呈正相关,与局部浸润单个核细胞亚群(MNC_s)的密度有一定相关。提示瘤细胞的MG_7抗原可能参与了肿瘤的局部免疫应答。     

LEISHMANIA PROMASTIGOTES EVADE INTERLEUKIN 12 INDUCTION BY MACROPHAGES AND STIMULATE A BROAD RANGE OF CYTOKINES FROM CD_（4）~（＋

ＬＥＩＳＨＭＡＮＩＡＰＲＯＭＡＳＴＩＧＯＴＥＳＥＶＡＤＥＩＮＴＥＲＬＥＵＫＩＮ１２ＩＮＤＵＣＴＩＯＮＢＹＭＡＣＲＯＰＨＡＧＥＳＡＮＤＳＴＩＭＵＬＡＴＥＡＢＲＯＡＤ　ＲＡＮＧＥＯＦＣＹＴＯＫＩＮＥＳＦＲＯＭ　ＣＤ_（４）~（＋）ＴＣＥＬＬＳＤＵＲＩＮＧＩ...     

甲状腺上皮细胞DR抗原异常表达及影响因素

本文应用单克隆抗体及免疫组化技术对37例自身免疫性甲状腺炎,23例甲亢及13例甲亢伴甲状腺炎甲状腺上皮细胞DR抗原异常表达进行原位研究。结果发现甲状腺上皮细胞DR抗原的异常表达与甲状腺浸润细胞总数、炎性破坏程度及外周血TSH、甲状腺球蛋白抗体、甲状腺微粒体抗体量呈正相关。     

乙型肝炎患者AMLR及非T细胞Ia抗原表达

本文用氚标胸腺嘧啶核苷,（~3H-TdR）掺入法检测102例各种类型乙肝病毒感染者自身混合淋巴细胞反应（AMLR）、并以单克隆抗体（McAb）Wu35（HLA-DR）和Wu22（HLA-DQ）,经PAP免疫酶组化技术测定非T细胞表面HLA-DR及HLA-DQ抗原。结果表明:除HBsAg携带者及慢性迁延性肝炎患者AMLR接近正常对照外,其它类型HBV感染者AMLR均有不同程度降低,尤其以重症肝炎和肝硬化降低明显。各类乙肝患者DR~+Mon和B细胞及DQ~+Mon和B细胞百分率均有不同程度的下降,以重症肝炎和肝硬化最显著。提示乙肝患者AMLR降低与刺激细胞（Mon和B细胞）表面DR和DQ抗原表达障碍有密切关系,与反应性T细胞无关。这可能是乙型肝炎疾病过程中细胞免疫功能障碍及免疫调节紊乱之机制所在。     

CLONING AND EXPRESSION OF A cDNA SEQUENCE FOR HUMAN THIOREDOXIN

Thioredoxin (TRX)isanoxidoreductaseen zyme,withamolecularweightof 1 2kD .Itwasi dentifiedoriginallyinE .coliasanhydrogendonorforribonucleotidereductaseanddeoxyribonucle otidesynthesis[1] .Thegeneofhumanthioredoxin(hTRX)islocatedonchromosome 3 p1 1 p1 2 ,withafulllengthof 1 3Kb .Theopenreadingframe ( 3 1 5nucleotideslong)codedforaproteinof 1 0 4aminoacids[2 ,3] .ManyfunctionsofTRXhavebeenre ported,whichincludethefollowing :TheTRXsystem (whichincludesHADPHasaprotondonor,TRXreductase ,…     

人B7.2 cDNA的克隆及其真核表达

为克隆人 B7.2 c DNA,构建 B7.2真核表达质粒 ,并在哺乳动物细胞中表达。分离正常人淋巴结淋巴细胞 ,经 LPS诱导后 ,以 RT- PCR,克隆 B7.2 c DNA;构建真核表达质粒 p BCMGSNeo- B7.2 ,转染哺乳细胞 ,进行表达。结果成功克隆了 B7.2c DNA,并经测序证实 :所构建的 B7.2抗原真核表达质粒可在哺乳动物细胞中高效表达。表明经 LPS诱导的人淋巴细胞可表达 B7.2 m RNA,所建立的 p BCMGSNeo-B7.2哺乳动物真核表达质粒 ,可供进一步研究 B7.2结构和功能。     

MOLECULAR CLONING OF HUMAN NEUROTROPHIN-4 GENE

Ｎｅｕｒｏｔｒｏｐｈｉｎ 4 (ＮＴ 4 )ｉｓａｍｅｍｂｅｒｏｆｈｅｎｅｕ ｒｏｔｒｏｐｈｉｎｆａｍｉｌｙｏｆｓｔｒｕｃｔｕｒａｌｌｙｒｅｌａｔｅｄ ｐｒｏｔｅｉｎｓ[1] ,ｔｈａｔａｌｓｏｉｎｃｌｕｄｅｓｎｅｕｒｏｔｒｏｐｈｉｎ 3(ＮＴ 3) ,ｎｅｕ ｒｏｔｒｏｐｈｉｎ 6 (ＮＴ 6 ) ,ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒ (ＮＧＦ)ａｎｄｂｒａｉｎ ｄｅｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ (ＢＤＮＦ) [2 - 6] ,ｗｈｉｃｈｓｕｐｐｏｒｔｓｔｈｅｓｕｒｖｉｖａｌｏｆｖｅｒｔｅｂｒａｔｅｎｅｕｒｏｎｓ[7] .Ａｔｐｒｅ…     

CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITSEXPRESSION IN ESCHERICHIA COLI

Objective Expressing and purifying the seglnent of SARS-CoV spike protein in E. Coli Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion (BamH Ⅰ , Pst Ⅰ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E. coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.     

EXPRESSION OF HUMAN RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST (rhIL-1ra) IN E.COLI

ThisprojectissupportedbyChineseMedicalBoard(U.S.A)In1990,EisenbergisolatedacDNAfromthehumanmonocytelibrary,expressionofthecDNAinE.coltyieldedtheproteinthatcouldhinderIL--lbindingIL--lreceptor,hetermedtheproteininterleukin--1receptorantagonist(IL--lra)ti.23.IL--lraisauniquecytokinethatcanstronglybindthecellsurfacereceptorsofIL--Iwithoutinducinganydetectableintracellularresponses"'.SinceitisaneffectivecompetitiveinhibitorofIL--lbothinvitroandinvivot'3,IL--lraisausefultooltodetermineth…     

CONSTRUCTION OF HUMAN INTERLUEKIN-18 DNA VACCINE AND IT‘S EXPRESSION IN MAMMALIAN CELLS

Ｈｕｍａｎｉｎｔｅｒｌｅｕｋｉｎ 18(ｈＩＬ 18)ｉｓａｎｅｗｌｙｉｄｅｎ ｔｉｆｉｅｄｃｙｔｏｋｉｎｅｍａｉｎｌｙ ｐｒｏｄｕｃｅｄｂｙｍｏｎｏｃｙｔｅｓａｎｄｍａｃｒｏｐｈａｇｅｓ.ＴｈｅｃｙｔｏｋｉｎｅｃａｎｉｎｄｕｃｅＴｈ1ｃｅｌｌｓｔｏｓｅｃｒｅｔｅＩＦＮ γ ,ＩＬ 2ａｎｄＧＭ ＣＳＦ .Ｉｔｅｎｈａｎｃｅｓｔｈｅｃｙ ｔｏｔｏｘｉｔｙｏｆＮＫａｎｄＣＴＬｃｅｌｌ,ａｎｄａｌｓｏｒｅｄｕｃｅｓｔｈｅｌｅｖ ｅｌｏｆＩＬ 10 .Ｔｈｕｓ,ｔｈｅｒｅｉｓｐｏｔｅｎｔｉａｌｔｈｅｒａｐｅｕｔｉｃｖａｌｕｅｏｆ…     

REGULATION OF PRODUCTION OF PARATHYROID HORMONE－RELATED PEPTIDE （PTHrP） AND EXPRESSION OF ITS mRNA IN A HUMANLIVER－DERIVED CE

ＲＥＧＵＬＡＴＩＯＮＯＦＰＲＯＤＵＣＴＩＯＮＯＦＰＡＲＡＴＨＹＲＯＩＤＨＯＲＭＯＮＥ－ＲＥＬＡＴＥＤＰＥＰＴＩＤＥ（ＰＴＨｒＰ）ＡＮＤＥＸＰＲＥＳＳＩＯＮＯＦＩＴＳｍＲＮＡＩＮＡＨＵＭＡＮＬＩＶＥＲ－ＤＥＲＩＶＥＤＣＥＬＬＬＩＮＥＬｉＨｕｓｏｎｇ；Ｐ...     

INTERFERON γ－INDEPENDENT EFFECTS OF INTERLEUKIN 12 ADMINISTERED DURING ACUTE OR ESTABLISHED INFECTION DUE TO LEISHMANIAMAJOR

ＩＮＴＥＲＦＥＲＯＮγ－ＩＮＤＥＰＥＮＤＥＮＴＥＦＦＥＣＴＳＯＦＩＮＴＥＲＬＥＵＫＩＮ１２ＡＤＭＩＮＩＳＴＥＲＥＤＤＵＲＩＮＧＡＣＵＴＥＯＲ　ＥＳＴＡＢＬＩＳＨＥＤＩＮＦＥＣＴＩＯＮＤＵＥＴＯＬＥＩＳＨＭＡＮＩＡＭＡＪＯＲＺｈｅｎｇＳｈｉｃｈｕｎ；Ｗ...     

ADENO-ASSOCIATED VIRUS INTRODUCED INTEGRATION AND EXPRESSION OF FOREIGN GENES IN PC12 CELLS

The ability to induce the expression of targetgenes in neurons is a goal of molecular neurobiolo-gy up to now. A variety of means including lipo-somes and recombinant retrovirus vectors and re-combinant adenovirus vectors have been used to ac-coplish this goal. Each method has some specificdisadvantages['J. None can provide reliable andcontinuouk gene expression in neurons of the cen-tral nervous system.Vectors derived from adeno--associaed virus(AAV ) have the potential to stably transducem…     

一种通用型重组2型腺伴随病毒载体的构建及外源基因表达的研究

以野生型2型人腺伴随病毒（AAV－2）基因组载体pSSV9－int-为基础，构建了一种携带和表达真核基因的基础AAV载体pSSV9／MulV－neosv，表达了neo基因，并采用该载体组建了携带人白细胞介素-2cDNA的重组AAV载体pSSV9／MulV－neosv－IL－2，转染A549细胞后表达了人白细胞介素-2基因。为应用腺伴随病毒作基因治疗研究提供了实验基础。     

胃肠癌的MHC抗原表达及其与局部免疫应答的关系

利用一组单克隆抗体对62例胃肠癌组织进行免疫组化研究。结果发现:HLA-ABC抗原缺失率在胃癌,结、直肠癌和局部淋巴结转移灶分别为26.92%、13.89%和33.33%;HLA-DR抗原表达率分别为34.62%、22.22%、和40.0%,而HLA-DQ抗原仅表达于2例HLA-DR胃癌的少数癌细胞。且HLA-ABC抗原缺失率在早期癌(60.0%)明显高于中晚期癌(15.79%);胃肠癌的HLA抗原表达与局部单个核细胞各亚群浸润的程度间也有一定的相关。这些发现提示HLA抗原可能在肿瘤发生发展过程中具有重要的免疫调节功能。     

bcl-2蛋白和PCNA在乳癌中表达的定量测定及其预后

应用图像分析仪对６０例乳癌ｂｃｌ－２、ＰＣＮＡ和雌孕激素受体进行免疫组织化学及酶联亲和法定量研究。结果表明：ｂｃｌ－２蛋白定量高值组,乳癌组织学分级低,患者生存期长、生存率高（Ｐ＜０．０５）;ＰＣＮＡ表达定量高值组,组织学分级高,患者生存期短、生存率低（Ｐ＜０．０５）。显示了定量检测ｂｃｌ－２蛋白和ＰＣＮＡ对评估乳癌分化程度及判断预后具有一定价值。ｂｃｌ－２与雌孕激素受体间有显著的正相关性（Ｐ＜０．００１）,提示ｂｃｌ－２蛋白检测对乳癌患者内分泌治疗有重要参考价值。     

两种酶结合物对棘球蚴三种抗原特异性及敏感性的检测比较

对９４例包虫病患者、４４例非包虫病患者及５０例健康人血清,用羊源林球蚴囊液、原头蚴及生发层抗原,分别进行了酶联金葡菌Ａ蛋白点免疫及酶标记单抗人ＩｇＧ点免疫试验,其结果显示：前者对三种抗原的敏感性分别为９４．７％、９５．７％和９６．８％;后者分别为９２．６％、９３．６％和９３．６％。前者的特异性分别为８１．９％、９２．６％和９２．６％;后者分别为８７．２％、９１．５％和９１．５％。酶联金葡菌Ａ蛋白点免疫除了林球蚴囊液抗原的特异性低于酶标羊抗人ＩｇＧ点免疫外,原头蚴抗原、生发展抗原的特异性和三种抗原的敏感性均比后者高。     

EXPRESSION OF PRE S ROTEIN OF HBV AS A ALKALINE PHOSPHATASE FUSION PROTEINS IN NIH 3T3 CELLS

ＥＸＰＲＥＳＳＩＯＮＯＦＰＲＥＳＲＯＴＥＩＮＯＦＨＢＶＡＳＡＡＬＫＡＬＩＮＥＰＨＯＳＰＨＡＴＡＳＥＦＵＳＩＯＮＰＲＯＴＥＩＮＳＩＮＮＩＨ３Ｔ３ＣＥＬＬＳＸｉａｏＳｈｅｎｇｘｉａｎｇ；Ｔ．Ｓ．Ｂ．Ｙｅｎ（ＬａｂｏｒａｔｏｒｙｏｆＭｏｌｅｃｕｌａｒＰａｔ...     

单纯疱疹病毒胸苷激酶基因表达对人肝癌细胞的生长抑制

使用转导有单纯疱疹Ⅰ型病毒胸苷激酶（ＨＳＶ１－ＴＫ）基因的人肝癌ＳＭＭＣ－７７２１细胞株建立了裸鼠移植瘤模型，并进行了在体和体外试验性无环鸟苷（ＡＣＶ）治疗。结果发现基因转导后的肝癌细胞表现出对ＡＣＶ的敏感性，肝癌细胞生长抑制与ＡＣＶ的剂量有关；用药组裸鼠除平均瘤重明显低于对照组外，还可见细胞核分裂指数降低，伴有细胞变性和坏死。结果表明：ＨＳＶ１－ＴＫ基因经逆转录病毒转导后，用ＡＣＶ诱导表达明显抑制了人肝癌细胞的生长。     

SUPPRESSION OF GROWTH OF A HUMAN LIVER－DERIVED CELL LINE BY ENDOGENOUSLY PRODUCED PARATHYROID HORMONE－RELATED PEPTIDE

ＳＵＰＰＲＥＳＳＩＯＮＯＦＧＲＯＷＴＨＯＦＡＨＵＭＡＮＬＩＶＥＲ－ＤＥＲＩＶＥＤＣＥＬＬＬＩＮＥＢＹＥＮＤＯＧＥＮＯＵＳＬＹＰＲＯＤＵＣＥＤＰＡＲＡＴＨＹＲＯＩＤＨＯＲＭＯＮＥ－ＲＥＬＡＴＥＤ　ＰＥＰＴＩＤＥＬＩＨｕｓｏｎｇ；Ｐ．Ｋ．Ｓｅｉｔｚ，Ｐ．...