Capillary electrophoresis coupled with electrochemiluminescence for determination of cloperastine hydrochloride

Objective To investigate the electrochemiluminescence (ECL) behavior of cloperustine hydrochloride.Methods ECL intensity of tris (2,2'-bipyridyl) rutheniumo(Ⅱ) was enhanced, the method for the determination of dupernstine hydrochloride was established using capillary electrophoresis (CE) coupled with electrochemilumolinescence (ECL) detection. Results Under the optimum conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0×10-6 g/mL to 1.0×10-4 g/mL. The detection limit (S/N=3) was 8.05×10-7g/mL. he relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0 ×10-5g/mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to eloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.     

Capillary electrophoresis with inhibited electrochemiluminescent detection for the trace analysis of epinephrine and dopamine

In this paper, a novel electrochemiluminescent (ECL) detection approach was developed for highly sensitive detection of ECL inhibitors based on the ECL inhibition of Ru(bpy)32+/2-(Dibutylamino)ethanol (DBAE) system. A microfluidic ECL detection cell was fabricated to couple with the capillary electrophoresis system, the electrochemical system and the postcolumn injection system. Both Ru(bpy)32+ and DBAE solutions were injected directly to the working electrode surface by a micro-infusion system to obtain a high and stable ECL signal. The performance of this setup was demonstrated by the analysis of two typical ECL inhibitors, dopamine and epinephrine. Under the optimal conditions, the limit of detection (LOD) for dopamine and epinephrine was 50nM and 5nM respectively. The proposed method was also successfully used for the trace analysis of dopamine and epinephrine in human serum samples.     

多敏灵片中咖啡因和盐酸多塞平含量的测定

目的建立高效液相色谱法,测定多敏灵片中咖啡因、盐酸多塞平的含量。方法采用高效液相色谱法,以Kromasil C18(250 mm×4.6 mm,5μm)为分析柱,流动相为甲醇-水-三乙胺(75∶25∶1),流速1 mL/min,检测波长295 nm,柱温为室温。结果咖啡因、盐酸多塞平与其他组分分离良好,且不受其他成分的干扰;咖啡因回归方程为:Y=196 604X+218 976(r=0.999 8,n=5),咖啡因在0.08~0.24μg的范围内线性关系良好;盐酸多塞平回归方程为:Y=60 158X+58 230(r=0.999 9,n=5),盐酸多塞平在0.04~0.12μg的范围内线性关系良好。方法的平均回收率分别为99.06%、96.67%,精密度(RSD)值分别为1.04%、1.13%。结论本方法操作简便、快速、准确、灵敏,适用于多敏灵片的含量测定。     

Normal and reverse flow injection-spectrophotometric determination of thiamine hydrochloride in pharmaceutical preparations using diazotized metoclopramide

Simple and sensitive normal and reverse flow injection methods for spectrophotometric determination of thiamine hydrochloride (THC) at the microgram level were proposed and optimized. Both methods are ...     

毛细相变流体回路中蒸发器的预热启动特性研究

通过分析毛细管内液柱的动量方程,从力平衡的角度获得毛细相变流体回路系统的启动判别条件,并建立蒸发器的三维导热模型来研究系统的启动特性.研究表明:蒸发器的启动预热时间随启动热流的增大而减小;减小液体补偿腔的高度可以加快蒸发器的启动过程,且启动热流愈小该现象愈显著;在较低的启动热流条件下,减小蒸气槽道的宽度,可以减小启动预热时间,改善蒸发器启动性能,而在较高的热流密度下,其对蒸发器的启动性能影响不大.     

应用离心造粒法制备盐酸二甲双胍微丸的研究

目的研究离心造粒粉末层积法制备盐酸二甲双胍微丸。方法采用多功能微丸包衣造粒机制备微晶纤维素空白丸芯和盐酸二甲双胍微丸,并且通过产率、粒径和粒径分布、堆密度、表面形态和圆整度、脆碎度和释放度对盐酸二甲双胍微丸进行质量评价。结果以盐酸二甲双胍∶乳糖∶微晶纤维素=100∶5∶3的比例,主机转速150 r/min,喷浆泵转速20 r/min,供粉速度25 r/min,滚圆6 min,进行制备微丸,获得了很好的效果。结论所制备的盐酸二甲双胍微丸圆整度好,粒径均匀,硬度合格,流动性好,释放完全,无骨架结构。     

Simultaneous determination of berberine, matrine and oxymatrine in traditional Chinese medicines by using nonaqueous capillary electrophoresis

A rapid method for the simultaneous determination of berberine (BBR), matrine (MT) and oxymatrine (OMT) by nonaqueous capillary electrophoresis (NACE) was developed. Optimum separation of the analytes was obtained on a 50cm×50μm i.d. fused-silica capillary using a non-aqueous buffer system of 70mM ammonium acetate, 7.0% acetic acid and 10% acetonitrile at 25kV and 20℃. The relative standard deviations (R.S.D.) of the migration times and peak areas of the three active components were 0.06%-0.20% and 0.12%-3.41% for berberine, 0.11%-0.60% and 0.74%-1.63% for matrine, 0.15% and 0.45% for oxymatrine, respectively. Detection limits of berberine, matrine and oxymtrine were 0.18μg/mL, 4.08μg/mL and 4.16μg/mL, respectively. In the tested concentration range, good linear relationships (0.9992 for berberine, 0.9988 for matrine and 0.9988 for oxymatrine) were observed. The linear calibration ranges were 0.45-360.0μg/mL for berberine, 8.16-408.0μg/mL for matrine and 20.8-416.0μg/mL for oxymatrine. This method has been successfully applied to the phytochemical analysis of alkaloids extracts from two commonly used traditional Chinese herbal drugs: Sophora flavescens Ait. (Kushen) and Cortex phellodendri chinensis (Huangbai) and their medicinal preparations.     

盾构隧道壁后注浆浆液毛细管渗透扩散模型

为探讨盾尾注浆扩散半径及管片所受注浆压力的计算方法,将浆液的扩散过程简化为其在土体中大量孔径不均匀的毛细管中的渗流运动,建立了浆液渗透扩散力学模型.基于柱面扩散理论,假定浆液为宾汉姆流体,引入等效孔隙率替代土体初始孔隙率,通过模拟浆液在单个毛细管中的渗透过程,得到了考虑浆液时效性的浆液扩散半径和管片所受浆液总压力的计算式.结合具体实例,讨论了浆液扩散半径、注浆对管片产生的压力与注浆压力和注浆时间的关系.分析结果表明:其他注浆参数相同时,在不同注浆压力和不同注浆时间条件下,浆液对管片产生的压力的增长速率均大于浆液扩散半径的增长速率;当盾尾建筑间隙影响厚度和土体等效孔隙率不变时,浆液流动锋面上毛细管总面积与浆液扩散半径成正比.      

轻轨车辆电气耦合轮对导向控制技术

针对独立车轮自导向性能差的问题,应用改进电轴技术在轻轨车辆电机单独驱动独立车轮中,形成一种电气耦合轮对型式,以无形的电轴替代传统的机械轴,改善导向性能.从电路分析理论出发,分析了新型耦合轮对的耦合性能和影响因素,确定电气耦合轮对是一种对左右车轮转角差自动施加反馈控制的技术,且轮对耦合的强弱可通过调节电容与电阻实现,调节电容方案因能耗低而优于调节电阻方案.建立电气耦合轮对和弹性阻尼耦合轮对轻轨车辆横向动力学模型,分析了轮对的转向行为.仿真结果表明:电气耦合轮对耦合力矩最大达到近1kN·m,耦合能力强;直线上受激扰后,2种车辆的横移量与摇头角自行趋于0,通过曲线时,横移量稳定值小于8 mm,摇头角小于0.2°,因此,2种耦舍轮对车辆具有直线对中能力和曲线通过性能;通过小曲线时,电机因电气耦合而造成的最大耗能功率低于140 W,系统能耗低.     

不同连接比例叶酸偶联壳聚糖的制备

目的 叶酸与壳聚糖偶联,并制备不同连接比例的叶酸偶联壳聚糖.方法 通过叶酸活性酯与壳聚糖上的氨基反应,制得叶酸偶联壳聚糖;采用红外光谱法验证偶联是否成功,紫外分光光度法测定每个壳聚糖上连接叶酸的量.结果叶酸偶联壳聚糖红外光谱图表明,叶酸与壳聚糖成功偶联,并制备了每个壳聚糖上连接叶酸的量大致为3个、10个、20个、30个的叶酸偶联壳聚糖.结论叶酸与壳聚糖成功偶联,并成功制备了不同连接比例的叶酸偶联壳聚糖.     

Liquid chromatography coupled with time-of-flight and ion trap mass spectrometry for qualitative analysis of herbal medicines

With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing g...     

适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术

目的建立适用于细粒棘球蚴囊液蛋白质组的双向电泳分离条件。方法冷冻干燥法制备囊液总蛋白提取物,观察不同的蛋白样品处理方法、IPG胶条pH范围和电泳上样量对双向电泳图谱的影响。结果经ReadyPrepTM2-D Cleanup Kit处理的提取物上样量为400μg,在pH7~10范围的胶条进行双向电泳分离,可获取蛋白斑点较多、重复性较好的二维电泳图谱。结论建立的细粒棘球蚴囊液双向电泳技术及图谱,可为细粒棘球蚴虫蛋白质组学的研究提供理论依据。     

弹性阻尼耦合轮对铁路客车系统横向稳定性

首次将弹性阻尼耦合轮对应用到客车系统中 ,并采用特征值法对该系统横向稳定性进行了分析。指出将弹性阻尼耦合轮对模型的扭转刚度和扭转阻尼分别取不同的值 ,可与阻尼耦合轮对、扭转弹性轮对以及独立轮对模型统一。研究结果表明 ,采用弹性阻尼耦合轮对模型 ,选取适当的扭转阻尼和扭转刚度 ,可以提高车辆蛇行临界速度。     

Sensing of chlorpheniramine in pharmaceutical applications by sequential injector coupled with potentiometer

This paper reports on development of a system consisting of a portable sequential injector coupled with potentiometric unit for sensing of chlorpheniramine （CPA）, based on the reaction of CPA with pota...     

跨座式单轨交通系统耦合振动特性

将列车的每节车厢简化为15自由度的动力系统, 由拉格朗日方程导出其振动微分方程, 将轨道梁简化为欧拉梁, 基于能量法和车辆与轨道梁位移协调条件, 建立了车辆和轨道梁耦合运动控制方程, 研究了跨座式单轨交通轨道梁的动力特性。计算了列车以不同车速通过时轨道梁的动力响应, 并比较了计算结果与实测结果。比较结果表明: 理论计算结果与实测结果基本吻合, 车速对轨道梁挠度的影响较小, 但对加速度影响较大, 加速度随车速增大而增大, 在40~50 km·h^-1处出现最大值, 之后随车速增大反而减小; 轮胎模型与轨道表面不平度功率谱密度函数对轨道梁横向响应计算结果影响较大, 使计算误差增大。     

Saliency motivated pulse coupled neural network for underwater laser image segmentation

The detection range of underwater laser imaging technology achieves 4—6 times of detection range of conventional camera in intervening water medium, which makes it very promising in oceanic research, deep sea exploration and robotic works. However, the special features in underwater laser images, such as speckle noise and non-uniform illumination, bring great difficulty for image segmentation. In this paper, a novel saliency motivated pulse coupled neural network(SM-PCNN) is proposed for underwater laser image segmentation. The pixel saliency is used as external stimulus of neurons. For improvement of convergence speed to optimal segmentation, a gradient descent method based on maximum two-dimensional Renyi entropy criterion is utilized to determine the dynamic threshold. On the basis of region contrast in each iteration step, the real object regions are effectively distinguished,and the robustness against speckle noise and non-uniform illumination is improved by region selection. The proposed method is compared with four other state-of-the-art methods which are watershed, fuzzy C-means, meanshift and normalized cut methods. Experimental results demonstrate the superiority of our proposed method to allow more accurate segmentation and higher robustness.     

硫酸盐盐渍土中毛细水上升规律的初探

在对盐渍土地区毛细水上升机理的研究过程中,首先论述了硫酸盐盐渍土中影响水分迁移的不同因素,其次通过室内试验,得出不同配比下硫酸盐盐渍土毛细水上升高度的基本规律,最后通过实验对硫酸盐盐渍土地区公路病害治理方面提出一些措施和建议。     

粘弹性地基上考虑耦合效应四边自由矩形薄板的非线性自由振动分析

基于Hamilton能量变分原理和薄板的基本假设,考虑地基阻尼的影响,建立了双参数粘弹性地基上考虑耦合效应的四边自由矩形薄板的非线性自由振动方程以及板域外的控制方程.应用瑞利-里兹法及循环迭代对方程进行求解,探讨了地基阻尼、地基弹性模量以及板的结构参数对双参数粘弹性地基上四边自由矩形薄板的非线性自由振动特性的影响,得出...