REVERSAL OF THE DOUBLE－STRANDED－RNA－INDUCED INHIBITION OF PROTEIN SYNTHESIS BY A CATALYTICALLY INACTIVE MUTANT OF THE PROTEIN

ＲＥＶＥＲＳＡＬＯＦＴＨＥＤＯＵＢＬＥ－ＳＴＲＡＮＤＥＤ－ＲＮＡ－ＩＮＤＵＣＥＤＩＮＨＩＢＩＴＩＯＮＯＦＰＲＯＴＥＩＮＳＹＮＴＨＥＳＩＳＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＭＵＴＡＮＴＯＦＴＨＥＰＲＯＴＥＩＮ　ＫＩＮＡＳＥＰＫＲＴｙｓｏｎ...     

THE EVALUATION OF THE EFFECT OF MEBENDAZOLE ON TREATING ASCARIS LUMBRICOIDES AND TRICHURIS TRICHURA INFECTION WITH TWO KINDS

ＴＨＥＥＶＡＬＵＡＴＩＯＮＯＦＴＨＥＥＦＦＥＣＴＯＦＭＥＢＥＮＤＡＺＯＬＥＯＮＴＲＥＡＴＩＮＧＡＳＣＡＲＩＳＬＵＭＢＲＩＣＯＩＤＥＳＡＮＤＴＲＩＣＨＵＲＩＳＴＲＩＣＨＵＲＡＩＮＦＥＣＴＩＯＮＷＩＴＨＴＷＯＫＩＮＤＳＯＦＴＨＥＲＡＰＹＷｅｉＲｕｎｍｉｎ...     

REGULATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASEPKR AND2＇5＇ OLIGOADENYLATE SYNTHETASE BY A CATALYTICALLY INACTIVE PKR M

ＲＥＧＵＬＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＡＮＤ２＇５＇ＯＬＩＧＯＡＤＥＮＹＬＡＴＥＳＹＮＴＨＥＴＡＳＥＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＰＫＲ　ＭＵＴＡＮＴＴＨＲＯＵＧＨ　Ｃ...     

COMPARATIVE STUDIES ON THE CORNEAL STRUCTURE OF MAMMAL ANIMALS：I．ULTRASTRUCTURE OF THE CORNEAL SUBSTANTIA PROPRIA IN GUINEA P

ＣＯＭＰＡＲＡＴＩＶＥ　ＳＴＵＤＩＥＳ　ＯＮ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＴＲＵＣＴＵＲＥ　ＯＦ　ＭＡＭＭＡＬ　ＡＮＩＭＡＬＳ：Ｉ．ＵＬＴＲＡＳＴＲＵＣＴＵＲＥ　ＯＦ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＵＢＳＴＡＮＴＩＡ　ＰＲＯＰＲＩＡ　ＩＮ　ＧＵＩＮＥＡ　Ｐ...     

THE NEURONS DOUBLE-LABELLED BY SEROTONIN AND GLUTAMATE-IMMUNOREACTIVITY IN

ＴＨＥＮＥＵＲＯＮＳＤＯＵＢＬＥ－ＬＡＢＥＬＬＥＤＢＹＳＥＲＯＴＯＮＩＮＡＮＤＧＬＵＴＡＭＡＴＥ－ＩＭＭＵＮＯＲＥＡＣＴＩＶＩＴＹＩＮＭＥＤＵＬＬＡＲＹＲＡＰＨＥＮＵＣＬＥＩＰＲＯＪＥＣＴＩＮＧＴＯＴＨＥＣＥＲＥＢＥＬＬＡＲＣＯＲＴＥＸＯＦＴＨＥＫＩ...     

MULTIPLE ENHANCER ELEMENTS MEDIATE THE INDUCTION OF C-FOS BY ANGIOTENSIN Ⅱ

ＭＵＬＴＩＰＬＥＥＮＨＡＮＣＥＲＥＬＥＭＥＮＴＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＢＹＡＮＧＩＯＴＥＮＳＩＮⅡＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．...     

MULTIPLE TRANS-ACTING FACTORS MEDIATE THE INDUCTION OF C-FOS IN VASCULAR SMO

ＭＵＬＴＩＰＬＥＴＲＡＮＳ－ＡＣＴＩＮＧＦＡＣＴＯＲＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．Ｎａｆｔｉｌａｎ（Ｄｅ...     

THE DISTRIBUTION OF ATRIONATRIURETIC POLYPEPTIDE （ANP）－LIKE SUBSTANCE IN THE HEART OF THE HUMAN FETUS AND RAT－AN IMMUNOHISTOC

ＴＨＥＤＩＳＴＲＩＢＵＴＩＯＮＯＦＡＴＲＩＯＮＡＴＲＩＵＲＥＴＩＣＰＯＬＹＰＥＰＴＩＤＥ（ＡＮＰ）－ＬＩＫＥＳＵＢＳＴＡＮＣＥＩＮＴＨＥＨＥＡＲＴＯＦＴＨＥＨＵＭＡＮＦＥＴＵＳＡＮＤＲＡＴ－ＡＮＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＳＴＵＤＹＬｅｉＴ...     

PHOSPHORYLATION OF THE MYELOID ZINC FINGER PRO TEIN MZF－1 IS DIFFERENTIALLY REGULATED DURING MYELOPOIESIS

ＰＨＯＳＰＨＯＲＹＬＡＴＩＯＮＯＦＴＨＥＭＹＥＬＯＩＤＺＩＮＣＦＩＮＧＥＲＰＲＯＴＥＩＮＭＺＦ－１ＩＳＤＩＦＦＥＲＥＮＴＩＡＬＬＹＲＥＧＵＬＡＴＥＤＤＵＲＩＮＧＭＹＥＬＯＰＯＩＥＳＩＳＨｕｉＰｅｉ；ＭａｒｉａＲ．Ｂａｅｒ，ＰｅｔｅｒＧ．Ｂｒａｄｆｏｒ...     

OBSERVATION OF THE EFFECT OF S－（－）USNIC ACID SODIUM ON TRICHOMONAS VAGINALIS BY TRANSMISSION ELECTRON MICROSCOPE

ＯＢＳＥＲＶＡＴＩＯＮＯＦＴＨＥＥＦＦＥＣＴＯＦＳ－（－）ＵＳＮＩＣＡＣＩＤＳＯＤＩＵＭＯＮＴＲＩＣＨＯＭＯＮＡＳＶＡＧＩＮＡＬＩＳＢＹＴＲＡＮＳＭＩＳＳＩＯＮＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＥＷｕＪｉｅ；ＺｈａｎｇＭｉｎｒｕ；ＤｉｎｇＤｏｎｇｎｉ...     

IN VITRO CO-STIMULATORY ACTIVITY OF HUMAN B7.2(IgV+C)PROTEIN PRODUCED BY ENGINEERED BACTERIA

It was well known that "two signals" wereboth necessary to activate T lymphocytes. The firstsignal was provided by TCR--CD, which was en-gaged with antigen peptide--MHC complex, and thesecond, also named the costimulatory signal, wasprovided by the interaction between costimulatorymolecules B7. l and B7. 2 on antigen presenting celland its counter--receptor CD28/CTLA4 on T cell.The first signal alone without costimulation willlead T cell to anergy or apoptosis['~'2. B7. 2 mayplay a cri…     

THE CONSTRUCTION AND EXPRESSION OF THE MURINE SCFV GENE IN E. COLI AGAINST HUMAN CERVICAL CANCER

Objective To obtain the gene of murine Single chain Fv fragment （ScFv） against haman cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridama cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDSPAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coll against haman cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen.     

TRANSCRIPTION ACTIVATION BY HBV PRE SI PROTEIN

ＴＲＡＮＳＣＲＩＰＴＩＯＮＡＣＴＩＶＡＴＩＯＮＢＹＨＢＶＰＲＥＳＩＰＲＯＴＥＩＮＸｉａｏＳｈｅｎｇｘｉａｎｇ；Ｔ．Ｓ．Ｂ．Ｙｅｎ（ＬａｂｏｒａｔｏｒｙｏｆＭｏｌｅｃｕｌａｒＰａｔｈｏｌｏｇｙ，ＤｅｐａｒｔｍｅｎｔｏｆＰａｔｈｏｌｏｇｙ，Ｕｎｉｖ．ｏｆ...     

EXPRESSION CLONING OF A PROTECTIVE LEISHMANIA ANTIGEN

ＥＸＰＲＥＳＳＩＯＮＣＬＯＮＩＮＧＯＦＡＰＲＯＴＥＣＴＩＶＥＬＥＩＳＨＭＡＮＩＡＡＮＴＩＧＥＮＥ．Ｍｏｕｇｎｅａｕ，Ｆ．Ａｌｔａｒｓ，Ａ．Ｅ．Ｗａｋｉｌ，ＺｈｅｎｇＳｈｉｃｈｕｎ；Ｔ．Ｃｏｐｐｏｌａ，Ｚ．Ｗａｎｇ，Ｒ．Ｗａｌｄｍａｎｎ，Ｒ．Ｍ．Ｌｏｃ...     

新型抗ErbB2、抗CD16三价双特异性抗体的构建及功能鉴定

目的构建一种新型三价抗ErbB2、抗CD16 BsAb。方法构建重组载体pET22b( )/BsAb;转化大肠杆菌BL21(DE3),获得重组蛋白的表达,通过包涵体复性纯化蛋白。应用悬浮培养系统扩增稳定表达抗人CD16 scFv-Fc融合蛋白的CHO细胞株(CG5细胞)和稳定表达抗人ErbB2 scFv-Fc融合蛋白的CHO细胞株(HG2细胞),通过rProtein A Sepharose Fast Flow亲和层析柱纯化融合蛋白,应用流式细胞术分析BsAb的结合能力。结果该BsAb可在大肠杆菌BL21(DE3)中以包涵体形式高效表达,通过对重组蛋白的复性可获得高纯度的蛋白;复性后的BsAb具有与其亲本抗体相似性的结合靶抗原的能力。结论新型三价抗ErbB2、抗CD16 BsAb能与高表达ErbB2的乳腺癌细胞系SKBR3细胞高亲和力结合和表达CD16的人外周血单个核细胞低亲和力结合。     

迟缓爱德华菌抗独特型单链抗体基因的构建、表达及鉴定

目的 构建、表达及鉴定迟缓爱德华菌抗独特型单链抗体基因.方法 采用RT-PCR方法从分泌迟缓爱德华菌独特型单克隆抗体的杂交瘤细胞株(1E11)中克隆出V_H和V_L可变区基因, 再通过重叠延伸拼接PCR方法在V_H和V_L可变区基因之间引入连接肽(Gly_4ser)_3,体外构建单链抗体基因;将其克隆至表达载体pET-28a并在大肠杆菌中表达,表达产物纯化后,用SDS- PAGE、Western blot及ELASA进行鉴定.结果 迟缓爱德华菌抗独特型单链抗体基因scFv在BL21(DE3)菌中获得表达,表达产物以不溶性包涵体形式存在,经过溶解包涵体、纯化和体外复性,获得了高纯度的单链抗体片段,重组蛋白的分子质量为27 ku.ELASA分析结果证实迟缓爱德华菌抗独特型单链抗体基因scFv与原代抗体一样,具有较高的抗原亲和力.结论 成功构建了迟缓爱德华菌抗独特型单链抗体基因scFv,为进一步制备迟缓爱德华菌抗独特型抗体基因工程疫苗奠定基础.     

REGULATED PHOSPHORYLATION OF THE GATA－2 DNA BINDINGPROTEIN IN ENDOTHELIAL CELLS

ＲＥＧＵＬＡＴＥＤＰＨＯＳＰＨＯＲＹＬＡＴＩＯＮＯＦＴＨＥＧＡＴＡ－２ＤＮＡＢＩＮＤＩＮＧＰＲＯＴＥＩＮＩＮＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＳＢｕＸｉｎＥ．Ｅ．Ｑｕｅｒｔｅｒｍｏｕｓ，Ｔ．Ｑｕｅｒｔｅｒｍｏｕｓ（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃｉｎｅ...