Bacterial production and microbial food web structure in a large arctic river and the coastal Arctic Ocean

Globally significant quantities of organic carbon are stored in northern permafrost soils, but little is known about how this carbon is processed by microbial communities once it enters rivers and is transported to the coastal Arctic Ocean. As part of the Arctic River-Delta Experiment (ARDEX), we measured environmental and microbiological variables along a 300 km transect in the Mackenzie River and coastal Beaufort Sea, in July–August 2004. Surface bacterial concentrations averaged 6.7 × 10⁵ cells mL^− 1 with no significant differences between sampling zones. Picocyanobacteria were abundant in the river, and mostly observed as cell colonies. Their concentrations in the surface waters decreased across the salinity gradient, dropping from 51,000 (river) to 30 (sea) cells mL^− 1. There were accompanying shifts in protist community structure, from diatoms, cryptophytes, heterotrophic protists and chrysophytes in the river, to dinoflagellates, prymnesiophytes, chrysophytes, prasinophytes, diatoms and heterotrophic protists in the Beaufort Sea.Size-fractionated bacterial production, as measured by ³H–leucine uptake, varied from 76 to 416 ng C L^− 1 h^− 1. The contribution of particle-attached bacteria (> 3 µm fraction) to total bacterial production decreased from > 90% at the Mackenzie River stations to < 20% at an offshore marine site, and the relative importance of this particle-based fraction was inversely correlated with salinity and positively correlated with particulate organic carbon concentrations. Glucose enrichment experiments indicated that bacterial metabolism was carbon limited in the Mackenzie River but not in the coastal ocean. Prior exposure of water samples to full sunlight increased the biolability of dissolved organic carbon (DOC) in the Mackenzie River but decreased it in the Beaufort Sea.Estimated depth-integrated bacterial respiration rates in the Mackenzie River were higher than depth-integrated primary production rates, while at the marine stations bacterial respiration rates were near or below the integrated primary production rates. Consistent with these results, P_CO2 measurements showed surface water supersaturation in the river (mean of 146% of air equilibrium values) and subsaturation or near-saturation in the coastal sea. These results show a well-developed microbial food web in the Mackenzie River system that will likely convert tundra carbon to atmospheric CO₂ at increasing rates as the arctic climate continues to warm.     

On the distribution of dissolved lead in the Loire estuary and the North Biscay continental shelf, France

The dissolved lead was studied in the whole salinity gradient of the system composed of the Loire estuary and the North Biscay continental shelf. About 130 samples were collected in winter 2001 and spring 2002 during Nutrigas and Gasprod campaigns (Programme PNEC-Golfe de Gascogne, RV Thalassa) and metal measurements were conducted on board by Potentiometric Stripping Analysis. In the Loire estuary, levels of dissolved lead ranged from 0.15 to 0.24 nM and from 0.04 to 0.26 nM in winter and spring, respectively. Compared to the concentrations reported in 1987 and 1990 (0.4–1.7 nM; Boutier, B., Chiffoleau, J.F., Auger, D., Truquet, I., 1993. Influence of the Loire river on dissolved lead and cadmium concentrations in coastal waters of Brittany. Estuar. Coast. Shelf S., 36:133–143, Estuarine, Coastal and Shelf Science 36, 133–143) our study indicated much lower values. The fall in concentration in the estuary could be attributed to the stopping of activity of Octel, a big manufacturer of tetra alkyl lead. Discharge in dissolved metal to the continental shelf by the Loire river was assessed as 7.5 and 1.9 kg day^− 1 for winter and spring, respectively. On the continental shelf, levels of dissolved lead varied within 0.06 and 0.27 nM in winter (0.15 ± 0.06 nM, sd = 1.96, n = 49), whereas concentrations measured in spring were in the range 0.06–0.17 nM (0.09 ± 0.03 nM, sd = 1.96, n = 60). This difference in metal concentration was related to the amounts of rainfall that have fallen over the continental shelf: estimations of inputs by this way (74 and 32 kg day^− 1 in winter and spring, respectively) appeared to be significantly higher than inputs from the Loire river (7.5 and 1.9 kg day^− 1 in winter and spring, respectively). The distributions of dissolved metal in the surface waters highlighted the role of suspended particular matter (SPM) for a rapid “trapping” of lead near the mouth of the estuary. The vertical distributions showed, in the stratified area, a biological transfer of lead between winter and spring from surface waters to the halocline.     

Picophytoplankton and nanophytoplankton abundance and distribution in the southeastern Beaufort Sea (Mackenzie Shelf and Amundsen Gulf) during Fall 2002

The distribution of picophytoplankton (0.2–2 µm) and nanophytoplankton (2–20 µm) in the Beaufort Sea–Mackenzie Shelf and Amundsen Gulf regions during autumn, 2002 is examined relative to their ambient water mass properties (salinity, temperature and nutrients: nitrate + nitrite, phosphate, and silicate) and to the ratio of variable to maximum fluorescence, Fv/Fm. Total phytoplankton and cell abundances (< 20 µm) were mainly correlated with salinity. Significant differences in picophytoplankton cell numbers were found among waters near the mouth of the Mackenzie River, ice melt waters and the underlying halocline water masses of Pacific origin. Picophytoplankton was the most abundant phytoplankton fraction during the autumnal season, probably reflecting low nitrate concentrations (surface waters average ~ 0.65 µM). The ratio Fv/Fm averaged 0.44, indicating that cells were still physiologically active, even though their concentrations were low (max Chl a = 0.9 mg m^− 3). No significant differences in Fv/Fm were evident in the different water masses, indicating that rate limiting conditions for photosynthesis and growth were uniform across the whole system, which was in a pre-winter stage, and was probably already experiencing light limitation as a result of shortening day lengths.     

Influence of the Mackenzie River plume on the sinking export of particulate material on the shelf

We examined the influence of the Mackenzie River plume on sinking fluxes of particulate organic and inorganic material on the Mackenzie Shelf, Canadian Arctic. Short-term particle interceptor traps were deployed under the halocline at 3 stations across the shelf during fall 2002 and at 3 stations along the shelf edge during summer 2004. During the two sampling periods, the horizontal patterns in sinking fluxes of particulate organic carbon (POC) and chlorophyll a (chl a) paralleled those in chl a biomass within the plume. Highest sinking fluxes of particulate organic material occurred at stations strongly influenced by the river plume (maximum POC sinking fluxes at 25 m of 98 mg C m^− 2 d^− 1 and 197 mg C m^− 2 d^− 1 in 2002 and 2004, respectively). The biogeochemical composition of the sinking material varied seasonally with phytoplankton and fecal pellets contributing considerably to the sinking flux in summer, while amorphous detritus dominated in the fall. Also, the sinking phytoplankton assemblage showed a seasonal succession from a dominance of diatoms in summer to flagellates and dinoflagellates in the fall. The presence of the freshwater diatom Eunotia sp. in the sinking assemblage directly underneath the river plume indicates the contribution of a phytoplankton community carried by the plume to the sinking export of organic material. Yet, increasing chl a and BioSi sinking fluxes with depth indicated an export of phytoplankton from the water column below the river plume during summer and fall. Grazing activity, mostly by copepods, and to a lesser extent by appendicularians, appeared to occur in a well-defined stratum underneath the river plume, particularly during summer. These results show that the Mackenzie River influences the magnitude and composition of the sinking material on the shelf in summer and fall, but does not constitute the only source of material sinking to depth at stations influenced by the river plume.     

Seasonal variation of riverine nutrient inputs in the northern Bay of Biscay (France), and patterns of marine phytoplankton response

Seasonal variations in nutrient inputs are described for the main rivers (Loire and Vilaine) flowing into the northern Bay of Biscay. The river plumes are high in N/P ratio in late winter and spring, but not in the inner plume during the summer. Conservative behavior results in most nutrients entering the estuary and eventually reaching the coastal zone. Temporal and spatial aspects of phytoplankton growth and nutrient uptake in the northern Bay of Biscay distinguish the central area of salinity 34 from the plume area. The first diatom bloom appears offshore in late winter, at the edge of the river plumes, taking advantage of haline stratification and anticyclonic “weather windows.” In spring, when the central area of the northern shelf is phosphorus-limited, small cells predominate in the phytoplankton community and compete with bacteria for both mineral and organic phosphorus. At that period, river plumes are less extensive than in winter, but local nutrient enrichment at the river mouth allows diatom growth. In summer, phytoplankton become nitrogen-limited in the river plumes; the central area of the shelf is occupied by small forms of phytoplankton, which are located on the thermocline and use predominantly regenerated nutrients.     

Physical and biological correlates of virus dynamics in the southern Beaufort Sea and Amundsen Gulf

As part of the Canadian Arctic Shelf Exchange Study (CASES), we investigated the spatial and seasonal distributions of viruses in relation to biotic (bacteria, chlorophyll-a (chl a)) and abiotic variables (temperature, salinity and depth). Sampling occurred in the southern Beaufort Sea Shelf in the region of the Amundsen Gulf and Mackenzie Shelf, between November 2003 and August 2004. Bacterial and viral abundances estimated by epifluorescence microscopy (EFM) and flow cytometry (FC) were highly correlated (r² = 0.89 and r² = 0.87, respectively), although estimates by EFM were slightly higher (FC = 1.08 × EFM + 0.12 and FC = 1.07 × EFM + 0.43, respectively). Viral abundances ranged from 0.13 × 10⁶ to 23 × 10⁶ ml^− 1, and in surface waters were ~ 2-fold higher during the spring bloom in May and June and ~ 1.5-fold higher during July and August, relative to winter abundances. These increases were coincident with a ~ 6-fold increase in chl a during spring and a ~ 4-fold increase in bacteria during summer. Surface viral abundances near the Mackenzie River were ~ 2-fold higher than in the Mackenzie Shelf and Amundsen Gulf regions during the peak summer discharge, concomitant with a ~ 5.5-fold increase in chl a (up to 2.4 μg l^− 1) and a ~ 2-fold increase in bacterial abundance (up to 22 × 10⁵ ml^− 1). Using FC, two subgroups of viruses and heterotrophic bacteria were defined. A low SYBR-green fluorescence virus subgroup (V2) representing ~ 71% of the total viral abundance, was linked to the abundance of high nucleic acid fluorescence (HNA) bacteria (a proxy for bacterial activity), which represented 42 to 72% of the bacteria in surface layers. A high SYBR-green fluorescence viral subgroup (V1) was more related to high chl a concentrations that occurred in surface waters during spring and at stations near the Mackenzie River plume during the summer discharge. These results suggest that V1 infect phytoplankton, while most V2 are bacteriophages. On the Beaufort Sea shelf, viral abundance displayed seasonal and spatial variations in conjunction with chl a concentration, bacterial abundance and composition, temperature, salinity and depth. The highly dynamic nature of viral abundance and its correlation with increases in chl a concentration and bacterial abundance implies that viruses are important agents of microbial mortality in Arctic shelf waters.     

Analysis of the hydrophysical structure of the Sea of Azov in the period of the bottom anoxia development

The hydrophysical and hydrochemical structure of the Sea of Azov, with developed bottom anoxia, was studied during the RV “Akvanavt” cruise from July 31 to August 03, 2001. The anoxic zone with a thickness from 0.5 to 4 m above the bottom was found in all deep regions of the Sea. Concentrations of hydrochemical parameters were similar to the pronounced anoxic conditions (about 90 mmol m^− 3 of hydrogen sulfide, 17 mmol m^− 3 of ammonia, 6 mmol m^− 3 of phosphate, 7 mmol m^− 3 of total manganese). The hydrophysical structure was characterized by the uniform distribution of temperature in the upper 6–7 m mixed layer (UML). Below this a thin (0.4–0.8 m) thermocline layer was observed, just above the anoxic waters. Formation of this phenomenon was connected with that summer weather conditions. Intensive rains led to increased influx of river waters in June. That resulted in large input of allochtonous organic matter (OM) and inorganic nutrients; the latter were consumed on the additional autochthonous organic matter production. In July the weather was characterized by a significant rise in the daily averaged air temperature and large oscillations of temperature during the day. In this period a wind of constant direction was absent, but wind bursts were observed. The completed analyses showed that the formation of such a structure could be connected with the following factors: (i) positive growth trends of the daily averaged temperature and the daily oscillations of temperature, (ii) presence of wind bursts. The joint action of these factors resulted in the formation of the UML. The amplitude of wind bursts determined the depth of UML, and the value of trend determined the value of the temperature change in the thermocline. An initial presence of bottom halocline (caused by the Black Sea water influx to the bottom of the Sea of Azov) prevented the heating of the bottom layer and therefore led to an increase of vertical gradient of temperature in the thermocline. The spatial distribution of the turbulent exchange coefficient confirmed the existence of a “stagnation” area located above the anoxia zone, which is also, apparently, the reason for its occurrence.     

Methane release and coastal environment in the East Siberian Arctic shelf

In this paper we present 2 years of data obtained during the late summer period (September 2003 and September 2004) for the East Siberian Arctic shelf (ESAS). According to our data, the surface layer of shelf water was supersaturated up to 2500% relative to the present average atmospheric methane content of 1.85 ppm, pointing to the rivers as a strong source of dissolved methane which comes from watersheds which are underlain with permafrost. Anomalously high concentrations (up to 154 nM or 4400% supersaturation) of dissolved methane in the bottom layer of shelf water at a few sites suggest that the bottom layer is somehow affected by near-bottom sources. The net flux of methane from this area of the East Siberian Arctic shelf can reach up to 13.7 × 10⁴ g CH₄ km^− 2 from plume areas during the period of ice free water, and thus is in the upper range of the estimated global marine methane release. Ongoing environmental change might affect the methane marine cycle since significant changes in the thermal regime of bottom sediments within a few sites were registered. Correlation between calculated methane storage within the water column and both integrated salinity values (r = 0.61) and integrated values of dissolved inorganic carbon (DIC) (r = 0.62) suggest that higher concentrations of dissolved methane were mostly derived from the marine environment, likely due to in-situ production or release from decaying submarine gas hydrates deposits. The calculated late summer potential methane emissions tend to vary from year to year, reflecting most likely the effect of changing hydrological and meteorological conditions (temperature, wind) on the ESAS rather than riverine export of dissolved methane. We point out additional sources of methane in this region such as submarine taliks, ice complex retreat, submarine permafrost itself and decaying gas hydrates deposits.     

Exchange of carbon by an upwelling filament off Cape Ghir (NW Africa)

We have studied the physicochemical and biological structure of a permanent filament off Cape Ghir (31°N) and estimated the transport of organic matter associated with it. The seaward filament exported coastal upwelled water, with low temperature and salinity and high organic matter, to the open ocean even in the absence of upwelling-favorable conditions. The estimated flux of excess organic carbon (the nonrefractory pool) expressed in annual basis yielded a value of 3.1×10⁹ kg C, from which 90% was transported as dissolved organic carbon. This flux represents about 63% of the average annual primary production for the region of study. We conclude that the net-offshore transport may contribute to the enrichment of offshore oligotrophic waters throughout the year, partly explaining the metabolic imbalance found in open ocean waters of the subtropical Northeast Atlantic.     

Suspended sediment and erosion dynamics in Kugmallit Bay and Beaufort Sea during ice-free conditions

The Mackenzie River is the largest river on the North American side of the Arctic and its huge freshwater and sediment load impacts the Canadian Beaufort Shelf. Huge quantities of sediment and associated organic carbon are transported in the Mackenzie plume into the interior of the Arctic Ocean mainly during the freshet (May to September). Changing climate scenarios portend increased coastal erosion and resuspension that lead to altered river-shelf-slope particle budgets. We measured sedimentation rates, suspended particulate matter (SPM), particle size and settling rates during ice-free conditions in Kugmallit Bay (3–5 m depth). Additionally, measurements of erosion rate, critical shear stress, particle size distribution and resuspension threshold of bottom sediments were examined at four regionally contrasting sites (33–523 m depth) on the Canadian Beaufort Shelf using a new method for assessing sediment erosion. Wind induced resuspension was evidenced by a strong relationship between SPM and wind speed in Kugmallit Bay. Deployment of sediment traps showed decreasing sedimentation rates at sites along an inshore–offshore transect ranging from 5400 to 3700 g m^− 2 day^− 1. Particle settling rates and size distributions measured using a Perspex settling chamber showed strong relationships between equivalent spherical diameter (ESD) and particle settling rates (r² = 0.91). Mean settling rates were 0.72 cm s^− 1 with corresponding ESD values of 0.9 mm. Undisturbed sediment cores were exposed to shear stress in an attempt to compare differences in sediment stability across the shelf during September to October 2003. Shear was generated by vertically oscillating a perforated disc at controlled frequencies corresponding to calibrated shear velocity using a piston grid erosion device. Critical (Type I) erosion thresholds (u) varied between 1.1 and 1.3 cm s^− 1 with no obvious differences in location. Sediments at the deepest site Amundsen Gulf displayed the highest erosion rates (22–54 g m^− 2 min^− 1) with resuspended particle sizes ranging from 100 to 930 µm for all sites. There was no indication of biotic influence on sediment stability, although our cores did not display a fluff layer of unconsolidated sediment. Concurrent studies in the delta and shelf region suggest the importance of a nepheloid layer which transports suspended particles to the slope. Continuous cycles of resuspension, deposition, and horizontal advection may intensify with reduction of sea ice in this region. Our measurements coupled with studies of circulation and cross-shelf exchange allow parameterization and modeling of particle dynamics and carbon fluxes under various climate change scenarios.     

Biological effects of Mississippi River nitrogen on the northern gulf of Mexico—a review and synthesis

The Mississippi River currently delivers approximately 1.82 Tg N year⁻¹ (1.3×10¹¹ mol N year⁻¹) to the northern Gulf of Mexico. This large input dominates the biological processes of the region. The “new” nitrogen from the river stimulates high levels of phytoplankton production which in turn support high rates of bacterial production, protozoan and metazoan grazing, and fisheries production. A portion of the particulate organic matter produced in the pelagic food web sinks out of the euphotic zone where it contributes to high rates of oxygen consumption in the bottom waters of the inner shelf, resulting in the development of an extensive zone of hypoxia each summer. In spite of the significance of this river system to the coastal ocean of the northern gulf, we do not have an adequate understanding of the inputs, processing and ultimate fates of river nitrogen. Here we review available literature on this important system and propose a conceptual model showing how biological processes evolve in the river plume between the point of discharge and the point where plume waters are fully diluted by mixing with oceanic water.     

Variations of the abundance and nucleic acid content of heterotrophic bacteria in Beaufort Shelf waters during winter and spring

Depth profiles of heterotrophic bacteria abundance were measured weekly over a 6-month period from December to May in Franklin Bay, a 230 m-deep coastal Arctic Ocean site of the southeastern Beaufort Sea. Total bacteria, low nucleic acid (LNA) and high nucleic acid (HNA) bacteria abundances were measured using flow cytometry after SYBR Green I staining. The HNA bacteria abundance in surface waters started to increase 5–6 weeks after phytoplankton growth resumed in spring, increasing from 1 × 10⁵ to 3 × 10⁵ cells mL^− 1 over an 8-week period, with a net growth rate of 0.018 d^− 1. LNA bacteria response was delayed by more than two months relative to the beginning of the phytoplankton biomass accumulation and had a lower net growth rate of 0.013 d^− 1. The marked increase in bacterial abundance occurred before any significant increase in organic matter input from river discharge (as indicated by the unchanged surface water salinity and DOC concentrations), and in the absence of water temperature increase. The abundance of bacteria below the halocline was relatively high until January (up to 5 × 10⁵ cells mL^− 1) but then decreased to values close to 2 × 10⁵ cells mL^− 1. The three-fold bacterial abundance increase observed in surface waters in spring was mostly due to HNA bacteria, supporting the idea that these cells are the most active.     

Vertical profiles of particulate organic matter and its relationship with chlorophyll-a in the upper layer of the NE Mediterranean Sea

Particulate organic matter (POM), nutrients, chlorophyll-a (CHL) and primary production measurements were performed in the upper layer of three different regions (cyclonic, anticyclonic and frontal+peripherial) of the NE Mediterranean Sea in 1991–1994. Depth profiles of bulk POM exhibited a subsurface maximum, coinciding with the deep chlorophyll maximum (DCM) established near the base of the euphotic zone of the Rhodes cyclone and its periphery, where the nutricline was situated just below the euphotic zone for most of the year. Moreover, the POM peaks were broader and situated at shallower depths in late winter–early spring as compared to its position in the summer–autumn period. Under prolonged winter conditions, as experienced in March 1992, the characteristic POM feature disappeared in the center of the Rhodes cyclone, where the upper layer was entirely occupied by nutrient-rich Levantine deep water. Deep convective processes in the cyclonic gyre led to the formation of vertically uniform POM profiles with low concentrations of particulate organic carbon (POC) (2.1 μM), nitrogen (0.21 μM), total particulate phosphorus (PP) (0.02 μM) and chlorophyll-a (0.5 μg/L) in the euphotic zone. Though the Levantine deep waters ascended up to the surface layer with the nitrate/phosphate molar ratios (28–29) in March 1992, the N/P molar ratio of bulk POM in the upper layer was low as 10–12, indicating luxury consumption of phosphate during algal production. Depth-integrated primary production in the euphotic zone ranged from 38.5 for oligotrophic autumn to 457 mg C m⁻² day⁻¹ for moderately mesotrophic cool winter conditions.     

Measuring the thicknesses of the freshwater-layer plume and sea ice in the land-fast ice region of the Mackenzie Delta using helicopter-borne sensors

Helicopter-borne sensors have been used since the early 1990s to monitor ice properties in support of winter marine transportation along the east coast of Canada. The observations are used in ice chart production and to validate ice hazard identification algorithms using satellite advanced synthetic aperture radar (ASAR) imagery. In this study we evaluated the sensors' additional capability to monitor the freshwater plume characteristic beneath land-fast ice. During the Canadian Arctic Shelf Exchange Study (CASES) data were collected over the Mackenzie Delta in the southern Beaufort Sea where a buoyant river plume exists. Results showed that the electromagnetic–laser system could describe not only the ice properties but also the horizontal distribution of the freshwater plume depths that decreased in depth stepwise offshore as the flow of the buoyant plume was restricted by a series of ridge-rubble fields running parallel to the coast. Relative to the 2 m mean ice thickness, the plume layer depth varied from zero under mobile offshore pack ice to 3 m inshore of the third set of ridge-rubble fields.     

Variations in ectoenzymatic hydrolytic activity in an oligotrophic environment (Southern Tyrrhenian Sea, W Mediterranean)

The variations in the expression of two hydrolytic ectoenzymes (leucine aminopeptidase – LA – and β glucosidase — BG) were studied in the southern Tyrrhenian Sea during spring 2004. This area is characterised by a complex morphology and hydrodynamism, which generate significant differences between different sectors, particularly in the 0–100 m layer. However, the area generally exhibits oligotrophic features such as low autotrophic pigment and organic matter concentrations and a higher bacterial biomass than the phytoplanktonic one. Despite this general bottom-up pressure, adaptations by the microbial consumers were indicated by the ectoenzymatic activities and by the relationships between the enzymes, their organic substrates and their producers (namely the bacteria). In particular, bacteria were able to exploit the inorganic N supply (nitrite + nitrate provided by irregular intrusions of intermediate waters) to escape the bottom-up limitation and produce enzymes such as BG devoted to the degradation of cellulose remnants and, therefore, also able to take advantage on this refractory organic matter. In the 200–800 m layer, where trophic limitation was strong due to the low values of potentially-labile organic matter (namely proteins), the peculiar hydrodynamism led to the formation of nepheloid layers rich in organic matter, which provided the bacteria with substrates and allowed the development of a significant correlation between LA activity and its own organic substrate. Furthermore, a reduction of the bottom-up pressure was also indicated by a higher mean bacteria cell size in the entire water column of the central and eastern sectors, and a significantly increased expression of BG related to the increase in the cell size.The ectoenzymatic activities, therefore, suggested that the southern Tyrrhenian Sea should be considered as a mosaic of subsystems, where the peculiar hydrological features stimulate bacterial adaptations and enhance the channelling of energy embedded in refractory materials into the food web.     

Characteristics and potential impacts of under-ice river plumes in the seasonally ice-covered Bothnian Bay (Baltic Sea)

The northernmost basin of the Baltic Sea, the Bothnian Bay, is ice-covered for about half the year. During this time, distinct under-ice river plumes develop, even seaward of the smallest rivers, that are substantially thicker and larger in extent than during the summer months. Wind mixing is negligible, and during late spring in April or May, the highest annual discharge occur while the sea is ice covered, thus providing conditions for the formation of extensive under-ice plumes. These plumes are characterised by high levels of trace elements (e.g., Al, Fe and Zn), organic matter (TOC and dissolved organic carbon [DOC]), nutrients and also optically active substances (colored dissolved organic matter, CDOM). The under-ice plumes provide an important pathway for undiluted transport of land-derived substances to the pelagic waters of the basin, affecting the salinity, chemistry and optical properties of coastal waters. Freshwater ice growth on the underside of an existing sea ice sheet also restricts the buildup of sea ice and under-ice algal communities, potentially in large areas along the coasts. Plume water influences the optical characteristics of coastal waters for a period of time after ice break-up, potentially affecting primary production in these areas. Furthermore, the formation of under-ice plumes potentially has a positive feedback on the ice season length due to freshening of the coastal waters (earlier freeze-up) and restricted oceanic heat flux (slower melting).     

Interannual variability of the shelf-slope front position between 75° and 50° W

The shelf-slope front (SSF) is a continuous shelf-break front running from the Tail of the Grand Banks to Cape Hatteras, North Carolina, separating colder and less-saline continental shelf waters from warmer and more saline slope waters. Time series containing mean monthly SSF positions were produced along each of 26 longitude lines between 75° and 50°W by workers located at Bedford Institute of Oceanography by digitizing individual frontal charts and computing mean monthly latitudinal positions over a 29-year (1973–2001) period. After removing seasonal variability at each longitude, interannual variability (IAV) of the SSF position at each longitude was computed as the annual mean of all monthly SSF position anomalies for each year over the 29-year period. Despite some missing data, a longitude-time plot reveals alternating bands of offshore (late-1970s, late-1980s, late-1990s) and onshore (early-1980s, early-1990s, early-2000s) annual mean SSF anomaly values, exhibiting a period of approximately 10 years. Annual mean SSF anomaly amplitudes are largest in the east, with maxima of O (± 100 km) located east of 60° W for years when data are available. Empirical orthogonal function (EOF) modes 1–4 (accounting for > 90% of the variance) form a set of basis functions that describe the SSF anomaly data and allow reconstruction of the entire data set since missing data are relatively few (14%). A complex empirical orthogonal function (CEOF) analysis using the “reconstructed” data reveals a wavelength scale of approximately 20° of longitude, a distance nearly equal to the entire study domain, along with steady, westward phase propagation of SSF anomalies over approximately the same distance. Speed calculations for the westward-propagating features yield a value of approximately 1.2 to 2.4 cm s^− 1 (1 to 2 km d^− 1), with annual mean SSF anomalies thus requiring about 4 years to propagate from the Tail of the Grand Banks in the east to Cape Hatteras, North Carolina, in the west. This propagation speed and the timing of the SSF positional anomalies at the Tail of the Grand Banks for the 29-year study period are in agreement with speeds computed for the propagation of quasi-decadal salinity anomalies through the Labrador Sea and the time of their arrival at the Tail of the Grand Banks. The small westward SSF anomaly propagation speed is an order of magnitude smaller than the associated currents, in agreement with a highly damped flow-through system originating from both Davis Strait and the West Greenland Current as discussed by other workers. Observations from both southern and northern portions of the study domain, within both continental shelf and slope waters, show that interannual changes in the volume of shelf water along with shelf water bulk properties exhibit a strong relationship with IAV of the SSF position over long time periods.     

Dissolved organic matter in shelf waters off the Ría de Vigo (NW Iberian upwelling system)

Net in situ production and export of dissolved organic carbon (DOC) and nitrogen (DON) have been studied in shelf waters off the Ría de Vigo (NW Spain), as part of a comprehensive hydrographic survey carried out from September 1994 to September 1995 with a fortnight periodicity. DOC and DON correlated well (r=+0.78), the slope of the regression line being 12.0±0.7 mol-C mol-N⁻¹, about twice the Redfieldian slope of particulate organic matter, 6.5±0.2 mol-C mol-N⁻¹ (r=+0.95). Labile DOC and DON accumulated in the upper 50 m during the upwelling season (March–September), mainly after prolonged periods of wind relaxation, when horizontal flows were reduced. This labile material represented 50% and 35% of the total (dissolved+particulate) organic carbon and nitrogen susceptible of microbial utilisation, which assert the key contribution of dissolved organic matter (DOM) to the export of new primary production in the NW Iberian upwelling system. This surface excess in shelf waters appeared to be formed into the highly productive Ría de Vigo (a large coastal indentation) at net rates of 4.4 μM-C d⁻¹ and 1.3 μM-C d⁻¹ in the inner and outer segments of the embayment respectively, and subsequently exported to the shelf. Once in the shelf, simple dilution with the inert DOM pool of recently upwelled Eastern North Atlantic Central Water (ENACW) occurred. Eventually, the DOM excess produced during the upwelling season is exported to the adjacent open ocean waters by the coastal circulation. Conversely, during the unproductive downwelling season (October–February), the lowest DOC and DON levels were recorded and export was prevented by the characteristic downwelling front associated to the seasonal poleward slope current.     

Inter-annual variability of hypoxic conditions in a shallow estuary

Water quality data from two monitoring programs in the Pamlico River Estuary (PRE) were analyzed for dissolved oxygen (DO), salinity, temperature, and nutrient concentrations. Data were collected bi-weekly at 8 stations from 1997 to 2003 by East Carolina University and continuously at three stations from 1999 to 2003 by the U.S. Geological Survey. Hypoxic conditions were observed mostly in the upper to middle estuary, but the frequency of hypoxic events varied between years. During June to October in 1997–1999 (referred to as the oxic summers) bottom water hypoxia (DO < 2 mg l^− 1) was found in 8.7% of the observations. By contrast, during June to October in 2001–2003 (referred to as the hypoxic summers), 37.9% of the total measurements had DO concentrations less than 2 mg l^− 1. The more frequent and/or prolonged hypoxic conditions during the hypoxic summers were closely associated with stronger salinity stratification and greater loadings of nutrient and particulate matter.Salinity stratification appeared to be governed by patterns of freshwater discharge, and frequency of wind mixing events. The “oxic” summers were characterized by continuous low freshwater inflow (except one extremely high flow event due to hurricanes), stronger northeastward wind, and more frequent wind mixing events. In contrast, the hypoxic summers were characterized by frequent moderate freshwater inflow events, and fewer wind mixing events.The greater loadings of nutrient (nitrate, ammonium, and phosphate) and particulate matter during the hypoxic summers were primarily due to higher river discharges. At the head of the PRE, no significant differences were found in concentrations of nutrient and particulate nitrogen between the oxic and the hypoxic summers. In addition, chlorophyll a concentrations were averaged above 30 μg l^− 1 (maximum 167 μg l^− 1) during the hypoxic summers, significantly higher than those during the oxic summers (averaged around 15 μg l^− 1).     

Validation of the 3D biogeochemical model MIRO&CO with field nutrient and phytoplankton data and MERIS-derived surface chlorophyll a images

This paper presents results obtained with MIRO&CO-3D, a biogeochemical model dedicated to the study of eutrophication and applied to the Channel and Southern Bight of the North Sea (48.5°N–52.5°N). The model results from coupling of the COHERENS-3D hydrodynamic model and the biogeochemical model MIRO, which was previously calibrated in a multi-box implementation. MIRO&CO-3D is run to simulate the annual cycle of inorganic and organic carbon and nutrients (nitrogen, phosphorus and silica), phytoplankton (diatoms, nanoflagellates and Phaeocystis), bacteria and zooplankton (microzooplankton and copepods) with realistic forcing (meteorological conditions and river loads) for the period 1991–2003. Model validation is first shown by comparing time series of model concentrations of nutrients, chlorophyll a, diatom and Phaeocystis with in situ data from station 330 (51°26.00′N, 2°48.50′E) located in the centre of the Belgian coastal zone. This comparison shows the model's ability to represent the seasonal dynamics of nutrients and phytoplankton in Belgian waters. However the model fails to simulate correctly the dissolved silica cycle, especially during the beginning of spring, due to the late onset (in the model) of the early spring diatom bloom. As a general trend the chlorophyll a spring maximum is underestimated in simulations. A comparison between the seasonal average of surface winter nutrients and spring chlorophyll a concentrations simulated with in situ data for different stations is used to assess the accuracy of the simulated spatial distribution. At a seasonal scale, the spatial distribution of surface winter nutrients is in general well reproduced by the model with nevertheless a small overestimation for a few stations close to the Rhine/Meuse mouth and a tendency to underestimation in the coastal zone from Belgium to France. PO₄ was simulated best; silica was simulated with less success. Spring chlorophyll a concentration is in general underestimated by the model. The accuracy of the simulated phytoplankton spatial distribution is further evaluated by comparing simulated surface chlorophyll a with that derived from the satellite sensor MERIS for the year 2003. Reasonable agreement is found between simulated and satellite-derived regions of high chlorophyll a with nevertheless discrepancies close to the boundaries.