陕西地区人群C_3的遗传多态性研究

用琼脂糖高压电泳继以免疫固定法对陕西地区273人补体第三组分(C_3)进行了分型.结果显示陕西地区人群中存在有两种C_3表型即SS、FS。其中SS的表型频率为98.53%,FS表型频率为1.47%,C_3S等位基因频率为0.9926,C_3F等位基因频率为0.0073。与国内其它汉族人群的分布一致,并与Hardy-weinbcng平衡相吻合。     

国人D17S30遗传多态性研究

采用基因扩增结合电泳银染技术分析１３０名汉族群体ＤＮＡＤ１７Ｓ３０遗传多态性，发现了１３个等位基因，基因频率为０．００７８～０．３５３８，有４２种基因型，其观察值和期望值经Ｘ２检验符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡定律；３个家系的９名成员Ｄ１７Ｓ３０遗传学分析，符合孟德尔遗传学规律。表明ＤＮＡＤ１７Ｓ３０在国人汉族群体具有高度多态性，在医学基因诊断、遗传学研究和法科学个人识别、亲权鉴定等领域是非常有价值的遗传标记。     

MITOCHONDRIAL DNA POLYMORPHISM IN CONTROL REGION FROM CHINESE YUGU POPULATION

Objective To investigate the mitochondrial DNA sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic data used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identified. The genetic diversity was calculated to be 0. 9691, and the genetic identity was calculated to be 0. 0406. Conclusion There are some particular polymorphism sites in Chinese YUGU ethnic group. The results suggest that sequence polymorphism from np]6091 -- 16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.     

ANALYSIS ON GENETIC POLYMORPHISM OF 6 STR LOCI ON CHROMOSOME 12 IN CHINESE HAN POPULATION

Shorttandemrepeats(STRs)arearichsource ofhighlypolymorphicmarkersinthehumange nome,arerelativelysmallinsize,andcanbestud iedwiththerelativeexpediently.ThustheSTR polymorphismsarehighlyusefultoolsforlinkagea nalysisofdisease relatedgenesandconstructionthe …     

ASSOCIATION ANALYSIS OF POLYMORPHISMS IN SIX GENES WITHIN THE GH/IGF-1 AXIS IN PATIENTS WITH IDIOPATHIC SHORT STATURE IN THE CHINESE HAN POPULATION

Objective To investigate relationships of polymorphisms in six genes ( GHR,IGF-1,IGF-1R,IGFBP-3,JAK2,and STAT5b) in the growth hormone ( GH)/insulin-like growth factor-1 (IGF-1) axis with idiopathic short stature (ISS) in the Chinese Han population. Methods A casecontrol study was carried out on a cohort of 198 ISS patients and 306 healthy controls.A total of 106 tagging single nucleotide polymorphisms (tagSNPs) from the six genes were selected from the HapMap ( haplotype map of the human genome ) Han Chinese in the Beijing subset.Results of genotyping conducted by highthroughput Illumina GoldenGateTM Assay were analyzed by statistical software. Results Both individual tagSNPs and haplotypes showed an association with ISS in the Hun Chinese population ( P ＜ 0.05 ).For each single test,both allele and genotype were tested.By allele frequency analysis,six positive SNP sites ( rsNo.1,rsNo.2,rsNo.3,rsNo.4,rsNo.5,and rsNo.6 ) of 3 genes ( JAK2,IGF-1R,and GHR) were found having associations with ISS. By genotype frequency analysis, there were significant differences between the patient and control groups in the following SNP sites:4 sites in JAK2 gene ( rsNo.1,rsNo.2,rsNo.3,and rsNo.4 ) and 1 site in GHR gene ( rsNo.6 ).The risk which affected ISS was found related to the JAK2 gene in 4 sites ( increase in rsNo.1 and decrease in rsNo.2,rsNo.3,and rsNo.4) and to the GHR gene in 1 site (decrease in rsNo.6).They were four haplotypes in gene of IGF-1R as “ TGC","CGCT",”TA",and "CA",one haplotype in IGFBP-3 as "TA",and one haplotype in JAK2 as "CTG",which revealed high significance for risks of affecting ISS. At last,multivariate logistic regression analysis of specific site rsNo.6 of the GHR gene revealed that the serum IGF-1 was related to genotypes AA and AC,with genotype CC as the reference ( P =0.015). Conclusion Genetic variances in six genes within the GH/IGF-1 axis may be important etiological factors for ISS in the Chinese Han population.     

USING STR POLYMORPHISM TO STUDY THE GENETIC RELATIONSHIPS AMONG A CHINESE KOREAN POPULATION AND OTHER GROUPS

Shorttandemrepeat(STR)polymorphismsare mainlyusedintheforensicfieldforpaternitytests andpersonalidentificationatpresent.Multiplex PCRamplificationandtypingsystemwithmulti coloredfluorescentlabeledprimershavemadeit convenienttogenotypeSTRsusingcommerciall…     

ENERGY CALCULUS IN CHINESE LANGUAGESEGMENTATION

IntroductionIn the past twenty years,many Chinese lan-guage processing systems have been developed.Excellent researchers[1～ 3] have led Chinese lan-guage processing into a new era.However,seg-mentation ambiguity problem has not beensolved satisfyingly.The current techniques to eliminate segmenta-tion ambiguity can be classified into two cate-gories:rule- based techniques( strong technique)and statistic- based techniques( soft technique) .Hybrid techniques belong to both.In rule- based techn…     

陕西汉族人群备解素因子B的遗传多态性研究

本文用琼脂糖凝胶高压电泳及免疫固定技术检测了326例陕西汉族人群的备解素因子B(简称Bf)的遗传多态性。在该人群中检出了BfSS、BfFS、BfFF及BfSS 07 4种Bf表型,他们的频率分别为0.7883,0.1687,0.0245及0.01 85。Bf的3个等位基因Bf~*F,Bf~*S和Bf~*S07频率分别为0.8819,0.1089及0.0092。这一结果与大部分已检测的中国汉族人群的Bf分布特点相似,而与白种人和黑种人的Bf多态性分布有所不同。     

THE GENE EXPRESSION OF BDNF IN NORMAL RABBIT RETINA

Objective To investigate the distribution of brain-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, also in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulation of RGCs.     

国人脑组织铝蓄积初步调查

本文系应用无火焰原子吸收光谱法测定了廿六年前和两年前的人脑灰质和白质中的铝含量,结果:两年前的脑灰质中含铝量比廿六年前的标本中含铝量高近两倍(分别为1.649和0.578),P<0.05,两时期脑白质中铝含量近似,P>0.05。     

小儿病毒性心肌炎脂质过氧化速率的变化

本文测定了71例病毒性心肌炎患儿血清脂质过氧化物、维生素E浓度和红细胞过氧化氢溶血率,发现脂质过物浓度和红细胞溶血率均高于正常组,脂质过氧化物亦高于上感组;脂质过氧化物浓度与红细胞溶血率呈轻度正相关,与乳酸脱氢酶活性中度正相关。急性期脂质过氧化物浓度和红细胞溶血率高于迁延期和慢性期,中重型高于轻型。提示自由基-脂质过氧化在此类心肌炎发病中起一定作用,且与病情和病期有一定关系。     

389例正常人骨矿物质含量分析

本文对３８９例正常人使用ＳＤ－１００单光子吸收骨密度测定仪进行左侧尺桡骨中下１／３交界处骨矿物质含量（ＢＭＣ）测定。结果显示各个年龄组男性ＢＭＣ明显高子女性，男女性ＢＭＣ高峰出现于３１岁～４０岁年龄组，男性在６１岁以后，女性在５１岁以后ＢＭＣ明显下降，男女性ＢＭＣ与体重有一定关系，但与身高没有相关性，本测定为该地区初步制定了一套正常人ＢＭＣ值。     

中国老年前期女性血沉参考值与地理因素的关系

为制定中国老年前期女性血沉参考值的统一标准提供科学依据,本文收集了中国各地用温氏法制定的健康老年前期女性血沉参考值,并对其与地理因素的关系进行了研究。发现海拔高度是影响老年前期女性血沉参考值最主要的因素,随着海拔高度的逐渐增大,老年前期女性血沉参考值在逐渐的减少,相关性很显著,用逐步回归分析的方法推导出了一个回归方程。可用之估算某地区的老年前期女性血沉参考值     

GENETIC POLYMORPHISM OF STR LOCI IN CHINESE DRUNGS

ＳＴＲｉｓａｕｎｉｖｅｒｓａｌｇｅｎｅｔｉｃｍａｒｋｅｒｔｈａｔｈａｓｃｈａｎｇｅａｂｌｅ ｐｏｌｙｍｏｒｐｈｉｓｍａｎｄｓｔａｂｌｅｈｅｒｅｄｉｔｙｉｎｈｕｍａｎ ｇｅｎｏｍｅ .ＩｔｉｓａｓｐｅｃｉｆｉｃＤＮＡｓｅｇｍｅｎｔｃｏｍｐｏｓｅｄｏｆ 2～ 7ｂａｓｅｐａｉｒｓａｓｉｔｓｃｏｒｅｓｅｑｕｅｎｃｅ ,ａｎｄｉｓｆｏｒｍｅｄｔｈｒｏｕｇｈｔｈｅｒｅｐｅａｔｅｄｃｏｎｎｅｃｔｉｏｎｏｆｔｈｅｓａｍｅｏｎｅ[1,2 ] .Ｓｉｎｃｅｉｔｈａｓｔｈｅｃｈａｒａｃｔｅｒｉｓｔｉｃｓｓｕｃｈａｓｎｕｍｅｒｏｕｓａ…     

翻译学视域下的西方涉华文论

西方主流媒体对日益崛起的中国所发生的一切都十分关注,大量刊载与中国相关的报道、评论.在涉华文章、评论的汉语用词翻译方面,形成了自己独特的方式,基本采用了异化框架下的音译、归化框架下的意译,文化融合中的音意兼译以及简译等手法.     

胰腺癌及其正常组织中糖胺聚糖的对比分析

用生化分析的方法对6例手术切除并证实为胰外分泌腺癌及其2例正常组织中糖胺聚糖的含量和组成成份进行对比分析。结果发现：①糖胺聚糖的合量癌组织显著高于正常胰腺组织；②双向电泳分析正常胰腺组织出现三条区带，为硫酸皮肤素、透明质酸和硫酸乙酰肝素，胰腺癌组织出现四条区带，除上述三种区带外还有明显的硫酸软骨素带；③超微结构示胰腺癌组织明显纤维化，肿瘤易玻坏基底膜向问质内浸润。结果提示在胰腺癌发生及发展中癌细胞和间质中糖胺聚糖的代谢和表达发生了显著的变化。     

TRANSVAGINAL COLOR DOPPLER STUDY OF UTEROPLACENTAL CIRCULATION IN NORMAL EARLY PREGNANCY

Ｔｈｅｐｈｙｓｉｏｌｏｇｉｃａｌｃｈａｎｇｅｓｏｆｕｔｅｒｉｎｅｈｅｍｏｄｙｎａｍｉｃｓｉｓｅｓｓｅｎｔｉａｌｆｏｒｃｌｉｎｉｃａｌｅｖａｌｕａｔｉｏｎｏｆｔｈｅｓｔａｔｕｓｏｆｔｈｅｕｔｅｒｉｎｅｃｉｒｃｕｌａｔｉｏｎ．Ｔｈｅｆｉｒｓｔｓｔｕｄｉｅｓｏｆｅａｒｌｙｕｔｅｒｏｐｌａｃｅｎｔａｌｂｌｏｏｄｆｌｏｗｗｅｒｅｃａｒｒｉｅｄｏｕｔｉｎｖｉｔｒｏｏｎａｂｏｒｔｉｏｎｔｉｓｓｕｅａｎｄｗｅｒｅｍａｉｎｌｙｒｅｓｔｒｉｃｔｅｄｔｏａｎａｔｏｍｉｃａｌｉｎｖｅｓｔｉｇａｔｉｏｎｏｆｔｈｅｕｔｅｒ…     

120例孕妇心理分析

对我院产前检查的１２０例健康孕妇进行了心理问卷调查，结果显示多数孕妇心理健康状况良好，认为生育是妇女的特权，因妊娠而心情愉快，全心期待执行优生优育政策而对胎儿性别无所谓，积极准备承担母亲角色。但有一些孕妇却存在不同程度的消极心理反应：忧虑、感情脆弱、依赖性强，为胎儿的健康与性别以及分娩痛苦而担心。针对目前孕妇的心理需求，提出了加强孕妇心理保健的措施。