多发性骨髓瘤患者氧自由基代谢状态的研究

测定了不同病期多发性骨髓瘤（MM）患者内源性抗氧化酶—红细胞超氧化物岐化酶（SOD）、谷胱甘肽过氧化物酶（GSH－Px）、过氧化氢酶（CAT）活性，血浆铜锌SOD（CuZn－SOD）、锰SOD（Mn－SOD）及总SOD（T-SOD）活性，血清铜、锌、锰、硒含量及血浆脂质过氧化物（LPO）含量。结果表明非缓解期MM患者红细胞3种抗氧化酶活性明显降低，血浆T－SOD、CuZn－SOD及Mn-SOD活性显著升高，血清锌、锰、硒含量降低，铜含量增高，血浆LPO含量明显增高。提示患者体内氧自由基含量增多，代谢增强，抗氧化能力明显减弱，且与疾病的发展及严重程度有关。     

多发性骨髓瘤患者超氧化物歧化酶和脂质过氧化物的改变及其意义

用邻苯三酚自氧化法和改良硫代巴比妥酸（ＴＢＡ）荧光微量法测定了多发性骨髓瘤（ＭＭ）患者初发期、缓解期和复发期红细胞超氧化物歧化酶（ＳＯＤ）活性和血浆脂质过氧化物（ＬＰＯ）含量。结果表明：ＭＭ初发和复发病例红细胞ＳＯＤ活性明显降低，血浆中ＬＰＯ含量明显增高，较正常对照均有明显差异。缓解期患者的ＳＯＤ活性和ＬＰＯ含量大致恢复正常水平。ＭＭ红细胞ＳＯＤ活力与血浆ＬＰＯ含量呈负相关（ｒ＝－０．４２５，Ｐ＜０．０５）），与血红蛋白浓度呈正相关（ｒ＝０．７２２，Ｐ＜０．００１），与骨髓中浆细胞百分数呈负相关（ｒ＝－０．５２６，Ｐ＜０．０５）。以上结果提示自由基损伤作用参与了ＭＭ的发生、发展和转归过程，且与病情的严重程度有关。     

神经内分泌分化与前列腺癌进展及预后的关系

目的研究神经内分泌分化(neuroendocrine differentiation,NED)与前列腺癌(prostate cancer,PCa)发展及预后的关系。方法收集有随访资料的61例PCa穿刺活检标本,采用免疫组化法检测嗜铬素A(chromogranin,CgA)、神经特异性烯醇化酶(neuron-specific enolase,NSE)、突触素(synaptophysin,Syn)在选定标本中的表达,分析NED与PCa各临床病理参数及患者生存时间的关系,多因素Cox风险模型分析影响PCa患者预后的危险因素。结果 61例PCa患者中有24例伴NED(39.3%),NED表达水平随着Gleason评分增加及穿刺前前列腺特异性抗原(prostatespecific antigen,PSA)水平升高而逐渐升高(P<0.01),Syn分别与NSE及CgA表达水平呈正相关性(P<0.01)。伴NED组PCa患者累积生存率明显低于不伴NED组(P<0.01),多因素Cox回归分析显示Gleason评分、NED是PCa患者生存的独立危险因素(P<0.01)。结论 NED与PCa发展及患者不良预后密切相关,是判断PCa患者预后的独立因素。     

THE EFFECT ON PROTEOGLYCAN METABOLISM OF DEOXYNIVALENOL AND SELENIUM IN THE CULTURED HUMAN FETAL CHONDROCYTES IN VITRO

Kashin BeckDisease(KBD)isasevere,en demicosteoarthrosis,butitspathogenesisisstilla pendentproblem.Withregardtoetiologyanalysis,mycotoxinhypothesisandlow seleniumhypothesis havebeenstudiedmoreinChina[1].Thepathologi calfeaturesofKBDarethedegenerationandnec…     

醛固酮及螺内酯对肝星状细胞中TIMP-1和MMP-2mRNA表达的影响

目的观察醛固酮及螺内酯对活化的肝星状细胞(HSC-T6)作用后不同时间段MMP-2和TI MP-1 mRNA的表达情况。方法采用RT-PCR的方法测定HSC-T6中MMP-2、TI MP-1 mRNA的表达。结果活化的HSC可表达MMP-2及TI MP-1的mRNA。醛固酮组MMP-2、TI MP-1的mRNA在48、72 h表达均高于对照组,在作用24、48、72 h时MMP-2、TI MP-1的mRNA的表达随时间递增。而螺内酯组则相反。醛固酮加螺内酯组与单加醛固酮组相比MMP-2、TI MP-1的mRNA表达下降。结论醛固酮可能通过促使MMP-2及TI MP-1 mRNA的表达促纤维化,螺内酯可抑制这一作用。     

NETWORK MANAGEMENT WITH SECURED MOBILE AGENT

IntroductionMobile Agents equipped with intelligence,of-fer new technology that helps automate NetworkManagement activities,which are increasingly be-coming complex due to exponential growth of in-ternet users,and thus demanding higher levels ofhuman manager expertise and involvement.Well-known network management protocols that areused to monitor and manage network devices in-clude Simple Network Management Protocol ( SN-MP) and Common Management Information Proto-col ( CMIP) ,SNMP be…     

EXPRESSION OF MATRIX METALLOPROTEINASE-9 IN HUMAN ABDOMINAL AORTIC ANEURYSMAL TISSUES

Objective To study the effects of MMP-9 （Matrix Metalloproteinase-9, MMP-9） in the pathogenesis of abdominal aortic aneurysms （AAAs） by localizing the expression of MMP-9 in the aneurysmal tissues. Methods By , the frozen sections （5μm） with aneurysmal tissues （n = 10） were incubated with MMP-9 antibody-added agents, then the sections were stained and observed under the microscope to localize the expression of MMP-9, which displayed a brown precipitate within the arterial walls. The normal arterial wall tissues（n= 10） and the diseased arterial wall tissues from the arterial occlusive diseases （AODs） （n= 15） were also immunized exactly the same way as control. Results A quantity of positive granules which appeared within the aortic media showed the strong expression of MMP-9 in the AAAs, with the positive rate reaching 95 % （19/20）, while no expression of MMP- 9 was observed in the normal artery. However, the scattered distributed positive granules were seen within the arterial wall of some cases of the AOI）s, implying the weak positive expression of MMP-9 in this disease with the positive rate of 26.7% （4/15）. There was a significant difference of the expression of MMP-9 within the arterial wall between the AAAs and AODs（P〈0.01 ）. Conclusion High expression of MMP-9 within the aortic media faciliatates the degradation of collagen and elustin fibres and subsequent dilation of the aortic artery , thus playing an important role in the pathogenesis of AAAs. To refrain MMP-9 from enhanced expressing within the aortic wall is of clinical significance in the prevention and treatment of AAAs.     

STUDY ON DETERMINATION AND APPLICATION OF THE SPECIFIC ANTIBODIES TO CYTOMEGALOVIRUS IN BONE MARROW TRANSPLANTATION

Cytomegalovirus(CMV)interstitialpneumonitis(lP)isoneofthemostseriouscomplicationsafterbonemarrowtransplantation(BMT).TheincidenceofCMV--lPisusually50%andthemortalityisalmost85%--100%".'>.AstherearenoeffectiveandspecifictherapeuticmeasuresforCMV-lP,ithasbecomethefirstimportantreasonforfailureofBMT.WaysofCMVinfectionaremainlybytrnasfusion,'organtransplantation,closecontactandverticaltransmission.PatientswithBMTareusuallysusceptibletoCMV.Thesepatientshavetoreceivetotalbodyirradiation(…     

层黏连蛋白及其受体在正常绒毛和绒癌组织中的表达及意义

目的探讨层黏连蛋白(LN)及层黏连蛋白受体(LN-R)与滋养细胞侵袭的关系。方法采用免疫组织化学的方法,观察正常早期妊娠绒毛和绒癌组织中LN、LN-R的表达。结果在正常绒毛组织中从绒毛、细胞柱到浸润的绒毛外滋养细胞,随着滋养细胞侵袭力的增加,LN及LN-R的表达呈下降趋势。绒癌组织的LN表达较正常绒毛明显减弱,两组间比较有显著性差异(P<0.05)。结论在滋养细胞侵袭的过程中,LN和LN-R起着重要作用。     

数据仓库在商品交易中的应用

着重讨论了数据仓库技术在商品交易中的应用，详细介绍了商品交易系统中数据仓库的设计和建立。并且进一步讨论了如何建立基于数据仓库的商品交易决策支持模型。     

松茸多糖对受照小鼠抗氧化系统的保护作用

目的探讨松茸多糖(PTM)对辐射损伤小鼠血清抗氧化系统的保护作用。方法 150只健康雄性ICR小鼠随机分为5组,正常对照组、辐射对照组和高、中、低3个剂量PTM组,每组30只。正常对照组和辐射对照组给予生理盐水灌胃,3个剂量PTM组给予不同剂量PTM灌胃,每天1次,连续7 d;第8天给予辐射对照组和PTM组动物全身一次性照射,总剂量2.0 Gy,检测小鼠照射后第1、5、14天的血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷光肝肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的变化。结果与辐射对照组比较,PTM组小鼠血清中SOD、CAT、GSH-Px活性明显升高,MDA含量明显降低(P<0.05或P<0.01)。结论松茸多糖对辐射损伤小鼠的血清抗氧化系统有显著保护作用。     

Liquid chromatography coupled with time-of-flight and ion trap mass spectrometry for qualitative analysis of herbal medicines

With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing g...     

INTRAUTERINE GROWTH RETARDATION AT FULL TERM PREGNANCIES WITH ENDOCRINE FACTORS

Intrauterinegrowthretardation(IUGR)isoneofthecriticalcomplicationsduringpregnancyandremainsthemajorcauseofperinatalmorbidityandmorta1ity.Despiteadvancesintheresearchofeti-ology,pathogenesis,diagnosis,andmanagementforIUGR,doctorscouldneitherdiagnoseitcor-rectlynormanageitefficiently.ThedataprovidedbynationalresearchinggroupforbirthdefectsshowedthattheincidencerateofIUGRwas6.38%inChina.Amongthetotalperinataldeathofneonates,themortalityofIUGRaccountsfor42.3%.Moreover,evenaftertheirbirth,th…     

Robust digital audio watermarking scheme using blind source separation with global optimal property

The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteris...     

Simple closed-loop method to compensate interference asymmetry in time-division duplex MIMO-OFDM systems

In wireless communications systems with time-division duplex (TDD) deployment, channel reciprocity and symmetric interference between transmitter and receiver sides are two widely-adopted assumptions for the design of optimal adaptation transmission mode. However, in practice, there is an undesirable but non-negligible effect, namely the asymmetric interference, that makes the assumptions no longer valid. In this paper, a simple closed-loop feedback method of compensating interference asymmetry in TDD multiple-input multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) system is proposed. The system makes the estimated interference at transmit-side be able to track the instantaneous receive-side interference dynamically. The proposed method maintains constant frame error rate (FER) by adopting adaptive modulation coding (AMC) and power loading. The final simulations have verified the effectiveness of the new method.     

A STUDY ON THE ALTERATION OF FREE RADICALS、PROSTAGLANDINS AND THEIR RELATIONSHIP IN CHILDREN EITH ACUTE GLOMERULONEPHRITIS

ＡＳＴＵＤＹＯＮＴＨＥＡＬＴＥＲＡＴＩＯＮＯＦＦＲＥＥＲＡＤＩＣＡＬＳ、ＰＲＯＳＴＡＧＬＡＮＤＩＮＳＡＮＤＴＨＥＩＲＲＥＬＡＴＩＯＮＳＨＩＰＩＮＣＨＩＬＤＲＥＮＥＩＴＨＡＣＵＴＥ　ＧＬＯＭＥＲＵＬＯＮＥＰＨＲＩＴＩＳＹａｎＸｉａｏｃａｉ；，ＷｅｉＪｕ...     

Guillain-Barre综合征早期电生理变化及分型再认识

分析了31例Guillain－Barre综合征（GBS）的电生理改变。有14例于病后3周行肌电图检查。早期运动神经传导速度（MCV）异常率为71．01％，主要表现为末端潜伏时（ML）延长，占61．22％，其次为MCV轻度减慢，而感觉神经传导速度（SCV）及肌电图（EMG）对早期GBS的诊断价值有限．但EMG鼻常出现越早，则预后越差。31例电生理特点分型：髓鞘型GBS27例（87．09％），轴索型GBS2例（6．4％），另有2例（6．4％）髓鞘及轴索损害均较重，预后差，故认为可能是两者同时损害的混合型。     

Guillain-Barre综合征早期电生理变化及分型再认识

分析了31例Guillain－Barre综合征（GBS）的电生理改变。有14例于病后3周行肌电图检查。早期运动神经传导速度（MCV）异常率为71．01％，主要表现为末端潜伏时（ML）延长，占61．22％，其次为MCV轻度减慢，而感觉神经传导速度（SCV）及肌电图（EMG）对早期GBS的诊断价值有限．但EMG鼻常出现越早，则预后越差。31例电生理特点分型：髓鞘型GBS27例（87．09％），轴索型GBS2例（6．4％），另有2例（6．4％）髓鞘及轴索损害均较重，预后差，故认为可能是两者同时损害的混合型。     

猫海马传入纤维的来源—HRP法研究

本文用8只雄性成年猫,以戊巴比妥钠麻醉,将33％的HRP(sigma Ⅵ)注射于右侧背海马或腹海马,在下列各核区观察到酶标细胞:丘脑前核,内、外侧下丘脑核,下乳头丘脑束核,上乳头体核,内侧隔核,斜角带,旁海马回,中缝背核,中央上核,篮斑和对侧海马的CA_3与CA_4区等。     

ABC—ELISA法检测自身免疫性甲状腺病患者TRAb的研究

本文叙述了在自身免疫性甲状腺病TRAb检测中引入一种新方法-ABC-ELISA法的研究过程。并将该法与常规ELISA法的检测灵敏度作了比较,用FRTL-5细胞测定TSAb的方法鉴定了该法的可靠法。未治甲亢用ABC—ELISA法测TRAb的阳性率91%,用常规ELISA法测TRAb阳性率70%,用FRTL-5细胞检测了未治甲亢中26例的TSAb所得结果与ABC法相同。据此,作者认为ABC—ELISA是一种敏感性高、重复性好、简便、适用于临床的方法。