EFFECT OF GRINDING TISSUE ON ISOLATE RECOVERY FROM RABBIT CORNEAS WITH FUNGAL KERATITIS

ＥＦＦＥＣＴＯＦＧＲＩＮＤＩＮＧＴＩＳＳＵＥＯＮＩＳＯＬＡＴＥＲＥＣＯＶＥＲＹＦＲＯＭＲＡＢＢＩＴＣＯＲＮＥＡＳＷＩＴＨＦＵＮＧＡＬＫＥＲＡＴＩＴＩＳＲｉｃｈａｒｄＲｏｂｉｎｓｏｎ，ＹａｎｇＲｏｎｇ；Ｗ．ＳｔｅｖｅｎＨｅａｄ，ＤｅｎｔｓＯ＇Ｄａｙ（Ｄ...     

PREVENTIVE EFFECT OF BORON ON DAMAGE TO CARTILAGE OF RATS WITH INTAKE EXCESSIVE FLUORIDE

Althoughfluorideinthebodymayhelpthe growthofbonesandteethinitsrangeofsafety,ex cessiveintakeoffluoridefromtheenvironmentcan damagetissuesandorgansinhumanandanimal, thusinducingskeletalfluorosis(SF)[1,2].Control lingoffluoridesources(suchastodefluoridate…     

STUDY ON LIQUID-LIQUID EXTRACTION OF RESVERATROL FROM GIANT KNOTWEED AND ITS PROTECTIVE EFFECT ON MYOCARDIUM INJURY

Ｒｅｓｖｅｒａｔｒｏｌｈａｓｂｅｅｎｆｏｕｎｄｉｎｍａｎｙｐｌａｎｔｓａｎｄｈａｓａｌｏｔｏｆｖａｌｕａｂｌｅｅｆｆｅｃｔｓｕｃｈａｓａｎｔｉ ｔｕｍｏｒ,ａｎｔｉ ｉｎｆｌａｍ ｍａｔｉｏｎ ,ｔｒｅａｔｍｅｎｔｏｆｃａｒｄｉｏ ｖａｓｃｕｌａｒｄｉｓｅａｓｅｓ[1] .Ｔｈｅｍｅｃｈａｎｉｓｍｉｓｒｅｌａｔｅｄｔｏｔｈｅｑｕｅｎｃｈｉｎｇｏｆｌｉｐｉｄｐｅｒｏｘｉｄａ ｔｉｏｎ[2 ,3] .Ｍａｎｙｐａｐｅｒｓｒｅｐｏｒｔｅｄｔｈａｔｒｅｓｖｅｒａｔｒｏｌｗａｓｅｘ ｔｒａｃｔｅｄｆｒｏｍｇｒａｐｅ .Ｈｏｗｅｖ…     

EFFECT OF SIBELIUM ON HYPOXIC CULTURED NEURONS FROM NEWBORN RATS CEREBRAL CONTEX IN VITRO

ＥＦＦＥＣＴＯＦＳＩＢＥＬＩＵＭＯＮＨＹＰＯＸＩＣＣＵＬＴＵＲＥＤＮＥＵＲＯＮＳＦＲＯＭＮＥＷＢＯＲＮＲＡＴＳＣＥＲＥＢＲＡＬＣＯＮＴＥＸＩＮＶＩＴＲＯ（刘小红）（陈征起）（和光祖）ＬｉｕＸｉａｏｈｏｎｇ；ＣｈｅｎＺｈｅｎｇｑｉ；ＨｅＧｕａｎｇｚｕ；...     

THE EFFECT OF HBsAg ANTIGENEMIA ON PREGNANCY

ＴＨＥＥＦＦＥＣＴＯＦＨＢｓＡｇＡＮＴＩＧＥＮＥＭＩＡＯＮＰＲＥＧＮＡＮＣＹＹｕｅＹａｆｅｉ；ＺｈａｎｇＳｈｕｌｉｎ；ＬＩＧｕｉｌｉｎ；（ＤｅｐａｒｔｍｅｎｔｏｆＯｂｓｔｅｔｒｉｃｓａｎｄＣｙｎｅｃｏｌｏｇｙ，ＦｉｒｓｔｃｆｆｉｌｉａｔｅｄＨｏｓｐｉ...     

INFLUENCE OF CHITOSAN ON CARBOHYDRATE METABOLISM IN EXERCISING MICE

Chitosan ,thealkalescencemacromoleculepoly mercontainingamidocyanogen ,canbeabsorbedbyhumanbodyandthebasicunitwasglucoseamine .Bothinternationalanddomesticstudiesdemonstratethatchitosancanpreventcellsfromoxygenationanditalsocaneliminatevarietiesoffreeradicalandre moveaciditymetabolismsubstanceseffectively[1,2 ] .However,therewasnoreportaboutwhetherchi tosanhadeffectonglucosemetabolisminthepro cessofexercising .Thisarticlemainlydiscusshowtoimproveeffectofchitosanonlucosemetabolismdisorderowingt…     

INHIBITION AND RADIATION SENSITIZING EFFECT OF INDOLEACETIC ACID COMBINED WITH HORSERADISH PEROXIDASE ON HELA CELLS

Indoleaceticacid (IAA )isagrowthhormoneforplants.ResearchinrecentyearshasshownthatIAApresentsacytotoxiceffectonhumancancersaf teritisoxidizedbyhorseradishperoxidase (HRP) .Becauseoflowtoxicityandstrongeffectonanaero biccells ,ithasattractedpeople sattentionasapro drugfortargettreatmentofcancers .ItsmechanismmightberelatedwiththefactthatIAAisoxidizedinthecellsofthelivingbodytoproducefreeradi cals.Theanaerobiccellgroupsincancersareoneofthemaincausesforradiationresistance .Therehavebeenfewr…     

EFFECT OF ROSIGLITAZONE AND METFORMIN ON CLOMIPHENE CITRATE RESISTANCE IN WOMEN WITH POLYCYSTIC OVARY SYNDROME

Objective To evaluate the impacts of rosiglitazone and metformin on ovarian response, hirsutism and insulin action in women with polycystic ovary syndrome (PCOS). Methods Ninety women resistant to clomiphene citrate with PCOS were randomized, 40 cases to rosiglitazone group, 50 cases to meformin group. Rosiglitazone and metformon were administered for 6 months in combination with clomiphene citrate on cycle day 5th to 9th, respectively. The clinical evaluations were performed monthly. Reproductive hormone, serum glucose and insulin levels were observed before and after treatment. The data were analyzed using repeated analysis of variance (ANOVA). Results There were significant changes in reducing hirsutism score, serum testosterone level, LH/FSH ratio, and restoration of ovarian ovulation after metformin or rosiglitazone administration respectively (P<0.05). Insulin sensitivity was more significantly improved in the rosiglitazone group than in metformin group (P<0.05) After treatment. Homa IR and Homa β were decreased from 1.54±0.34 to 0.83±0.38 and from 5.83±0.55 to 4.95±0.54 (P<0.05) in the rosiglitazone group. The body mass index(BMI) was decreased from 25.30±3.64 to 23.83±2.32 in the metformon group (P<0.05). There were no significant changes in ovulation rate and pregnancy rate between groups (P>0.05). Conclusion Rosiglitazone can increase insulin sensitivity. Metformon may reduce BMI. They all restore regular menstrual cycles, increase pregnancy rate, and reduce testosterone and LH concentration in women with polycystic ovary syndrome.     

THE EVALUATION OF THE EFFECT OF MEBENDAZOLE ON TREATING ASCARIS LUMBRICOIDES AND TRICHURIS TRICHURA INFECTION WITH TWO KINDS

ＴＨＥＥＶＡＬＵＡＴＩＯＮＯＦＴＨＥＥＦＦＥＣＴＯＦＭＥＢＥＮＤＡＺＯＬＥＯＮＴＲＥＡＴＩＮＧＡＳＣＡＲＩＳＬＵＭＢＲＩＣＯＩＤＥＳＡＮＤＴＲＩＣＨＵＲＩＳＴＲＩＣＨＵＲＡＩＮＦＥＣＴＩＯＮＷＩＴＨＴＷＯＫＩＮＤＳＯＦＴＨＥＲＡＰＹＷｅｉＲｕｎｍｉｎ...     

多抗甲素对小鼠腹腔巨噬细胞的作用

本文研究多抗甲素对S_(180)荷瘤小鼠腹腔巨噬细胞的作用,结果表明,多抗甲素能促进巨噬细胞定向趋化及活化强度,并提高其吞噬鸡红血球和产生TNF_α能力。提示增强单核—巨噬细胞吞噬能力和TNF_α细胞毒作用可能是多抗甲素抗肿瘤作用的一个主要机制。     

促肝细胞生长素对小鼠肝纤维化的影响

用四氯化碳致小鼠肝纤维化，分别用促肝细胞生长素（PHGT）和复方丹参注射液进行实验治疗。结果显示：实验治疗组病理显示肝细胞变性坏死经，胶原纤维形成减少，肝细胞再生显著，和复方丹参注射液组相比，无显著差异；透明质酸检测肝纤维化模型组含量明显高于治疗组，而治疗组和对照组相比，无显著差异（P＞0．05）。提示PHGT有抗肝纤维化作用，PHGT对小鼠肝纤维化作用的影响主要是肝细胞得到较好的保护，减轻CCl4造成的肝细胞损伤，终止肝细胞变性坏死，抑制肝纤维化的启动因素，使肝细胞再生。     

经腹化疗栓塞治疗肝癌的疗效分析

我院自1988年～1993年经腹手术行肝癌化疗栓塞122例,手术死亡4例,余全部随访。1年生存率31％,2年生存率6.5％。肿瘤直径在10cm以下者1年生存率75％,膨胀型生长的病人1年生存率63.15％。合并重度肝硬化的病人1年生存率26.3％。肝动脉门静脉双栓塞的病人1年生存率53.3％。结果表明对于中晚期肝癌的病人尽管病变广泛,肝硬化严重,但治疗应取积极态度,经血管途径的化疗栓塞手术有明显的姑息治疗效果。     

硒对实验性肝损伤保护作用的研究

采用综合法建立慢性肝损伤动物模型，分组观察硒对实验性肝损伤的影响。结果表明：①补硒组谷丙转氨酶降低，血清白蛋白回升，肝组织病理改变减轻；②补硒组动物肝组织硒含量及谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性显著增高（Ｐ＜０．０１）；③超微结构显示补硒组比对照组也有明显改善。表明硒具有保护肝损伤和促进肝细胞再生的作用。     

硒对大鼠肝核糖体含量及其聚集程度的影响

观察了低硒组和加硒组大鼠肝去线粒体上清液（ＰＭＳ）中总核糖体含量、多聚核糖体／总核糖体比值的变化。同时还测定了肝匀浆中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性、核酸含量及ＴＢＡ值。结果表明：低硒组大鼠肝ＰＭＳ中总核糖体含量及其聚集程度均显著低于加硒组，肝ＧＳＨ－Ｐｘ活性和ＲＮＡ含量也明显低于加硒组，而肝ＴＢＡ值升高。     

加味当归补血汤对环磷酰胺注射后小鼠免疫功能的影响

加味当归补血汤（MDB）连续胃饲小鼠10d（50mg／d），观察其对环磷酰胺所致的免疫抑制小鼠免疫功能的影响。结果显示：MDB对免疫受抑小鼠红细胞免疫功能（以C3b受体花环率和IC花环率为指标）及细胞免疫功能（以E-玫瑰花结形成、酸性α-醋酸萘酯酶和淋巴细胞转化率为指标）均有明显恢复和增强作用，而对体液免疫功能（以血清凝集素和脾溶血空斑形成细胞为指标）作用不显著。     

补硒对大骨节病患者红细胞免疫功能的影响

给１３～１６岁大骨节病患者每日口服２００μｇ亚硒酸钠硒或硒酵母硒１２周，测定红细胞硒含量和谷胱甘肽过氧化物酶（ＧＰＸ）活性、红细胞免疫粘附（ＲＣＩＡ）功能、血清ＲＣＩＡ调节功能和循环免疫复合物（ＣＩＣ）含量，以探讨补硒对大骨节病患者ＲＣＩＡ功能的影响。结果表明：补硒后患者的红细胞硒含量、ＧＰＸ活性和红细胞Ｃ３ｂ受体（ＲＣ３ｂＲ）花环率均明显提高，而血清ＲＣＩＡ抑制率明显降低，对红细胞免疫复合物（ＩＣ）花环率、ＣＩＣ含量及血清ＲＣＩＡ促进率无明显影响。硒酵母硒提高血硒和ＲＣＩＡ功能的作用比亚硒酸钠硒明显。说明：补硒可使病区大骨节病患者ＲＣＩＡ功能和血清ＲＣＩＡ调节功能恢复。     

硒对饲克山病病区粮大鼠肝线粒体~3H—亮氨酸参入的影响

本文采用同位素活体参入的方法,观察了饲克山病病区粮组和加硒粮组大鼠肝~3H—亮氨酸参入比放射性的变化,同时测定了肝硒含量,谷胱甘肽过氧化物酶活性和TBA值。发现病区组大鼠肝线粒体参入率,谷胱甘肽过氧化物酶活性和肝硒含量明显降低,而TBA值却显著升高。此改变提示,病区组大鼠肝抗过氧化能力低下,脂质过氧化物及其代谢产物堆积,导致线粒体蛋白质合成障碍,这可能是内环境缺硒时动物组织细胞呼吸酶活性降低,能量代谢障碍的重要原因之一。     

益肾降脂片对实验性肾阳虚小鼠IgG含量的影响

用益肾降脂干浸膏给正常小鼠灌胃，其ＩｇＧ含量无明显变化（Ｐ＞０．０５）。而对用氢化考的松制造的小鼠肾阳虚模型，则ＩｇＧ含量明显增加（Ｐ＜０．０５），表明益肾降脂片能提高肾阳虚小鼠的体液免疫功能。