MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

Objective To examine the differentially expressed invasion-related genes in two anchorage-independent uterine cervical carcinoma cell lines derived from the same patient using a cDNA array. Methods Two human uterine cervical carcinoma subclonal cell lines CS03 and CS07 derived from a single donor line CS1213 were established by limited dilution procedure. The two cDNA samples retro-transcribed from total RNA derived from CS03 and CS07 cells were screened by a cDNA microarray carrying 234 human cell-cycle related genes and 1011 human signal transduction and membrane receptor -associated genes, scanned with a ScanArray 3000 laser scanner. Results The cDNA microarray analysis showed that ]2 genes in CS03 were up-regulated compared to CS07, and 24 genes in CS07 were upregulated. The function of a number of differentially expressed genes was consistently associated with cell-cycle, cell proliferation, migration, apoptusis, signal transduction and tumor metastasis, including p34^cdc2, TSC22, plasminogen activator inhibitor Ⅰ （PAI-1）and desmusome associated protein（Pinin）. Conclusion Multiple genes are differentially expressed in uterine cervical carcinoma cell lines even came from the same patient. It is suggested that these genes are involved in the different phenotypic characteristics and development of cervical carcinoma.     

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合理的配送路线可以提高物流配送的效率。启发式的最优路径选择方法是目前研究的热点，本文针对模拟退火算法收敛速度慢的缺点，提出采用退火过程的导引知识（即上次退火过程节点的分布情况）作为下次选择节点的依据，从而提高模拟退火算法的速度。分别针对不同的配送节点进行实验，结果表明，基于导引知识的模拟退火算法能够获得更快的收敛速度，更快得到最优解。     

不同表型的人宫颈癌细胞亚克隆株的基因表达谱

目的　采用基因芯片技术分析同一人宫颈癌细胞株来源 ,锚着依赖性不同的两个亚克隆细胞株的侵袭相关基因表达。方法　采用有限稀释法建立CS12 13细胞的CS0 3和CS0 7亚克隆细胞株 ,检测其在软琼脂中的集落形成率 ,分别用流式细胞术和免疫组化法观察细胞黏附分子CD4 4和E cadherin、Fibronetin的表达 ,并将CS0 3和CS0 7细胞的总RNA经逆转录获得的cDNA作为探针 ,与含 10 11个与细胞信号转导和细胞膜受体相关基因表达谱芯片杂交 ,信号用ScanArray 30 0 0扫描分析。结果　CS0 3和CS0 7细胞的集落形成率分别是 0和 38.5 % ,CD4 4表达分别是 35 .1%和 13.7% ;两者的基因表达谱差异明显 ,CS0 3细胞中表达上调的基因为 12个 ,CS0 7细胞中有 2 1个基因表达增加 ,其中一些与细胞增生、迁移、凋亡 ,细胞间信号传导和肿瘤转移相关 ,包括p34cdc2 、转化生长因子 β刺激蛋白 (TSC2 2 )、纤维蛋白酶原活性因子抑制剂 (PAI 1)和桥粒相关蛋白 (PININ)等。结论　同一来源的人宫颈癌细胞株中有多个差异表达基因 ,它们参与人宫颈癌细胞的表型特征 ,可能影响肿瘤的进展。     

A Hybrid TCNN Optimization Approach for the Capacity Vehicle Routing Problem

Introduction The vehicle routing problem(VRP)is one ofthe well-known NP-hard optimization problems[1]occurring in many transport logistics and distribu-tion systems of considerable economic significance.The capacity vehicle routing problem(CVRP)likeVRP with the additional constraint that every vehi-cle must have a capacity,so it is also an NP-hardoptimization problems.It involves minimizing costvehicle schedules for a fleet of vehicles originatingand terminating from a central depot.The …     

风化砂岩在路基施工中的应用研究

在有些山区存在大量的风化砂岩,同时山区土地资源缺乏,如果能因地制宜地使用风化砂岩材料,将会带来节约土地和保护环境的社会效益和经济效益。结合风化砂岩在施工中的应用经验,从若干方面论述风化砂岩的物理性质、基本特性以及在公路路基施工中的应用方法,可为公路技术人员提供参考。     

物料配送和废旧产品回收的VRPSDP问题的并行模拟退火算法

为了求解面向制造领域物料配送和废旧产品回收的同时送取货的车辆路径优化问题（VRPSDP）,提出一种主从式并行模拟退火算法代替传统的串行模拟退火算法．算法首先使用剩余承载力和径向附加费（RCRS）算法求得初始解,然后在并行模拟退火算法框架下使用4种邻域搜索方法对初始解进行优化．最后,选取国际上通用的Montane和Galvao测试数据集（18个顾客数为100～400的测试问题）对算法性能进行测试,与文献中其他5种算法的比较显示,提出的并行模拟退火算法总体性能较优．     

人体桡神经浅支支配机械感受性单位的生理学特征

实验用神经内微记录和微刺激相结合的方法,对人体桡神经浅支支配的有毛皮肤所属机械感受性单位的特征和诱发感觉的性质进行了观察。在47个感觉单位中,SA单位占74.5%,其中SAⅠ61.7%,SAⅡ12.8%,RA单位占25.5%,没有发现毛囊单位和PC单位。SA单位的平均机械阈值高于RA单位,RA和SAⅠ单位的感受野明显小于SAⅡ单位。神经内微刺激诱发感觉的性质取决于所兴奋单位的类型,RA单位产生间断的跳动感,SAⅠ单位产生持续的压感;而SAⅡ单位在单独兴奋时不产生特定的感觉。结果提示:人体桡神经浅支支配的有毛皮肤机械感受性单位的特征与无毛皮肤没有明显差别。     

基于卡尔曼滤波的快时变稀疏信道估计新技术

针对高铁以及山区环境下正交频分复用（orthogonal frequency division multiplexing,OFDM）通信系统的信道估计问题,提出一种基于卡尔曼滤波的快时变稀疏信道估计方法.该方法基于快时变信道的基扩展模型（basic expansion model,BEM）,应用压缩感知（compressed sensing,CS）理论进行稀疏时延估计,并应用卡尔曼滤波（Kalman filter,KF）技术对BEM系数进行估计,进而获得信道增益.仿真结果表明,在相同信噪比（signal to noise ratio,SNR）条件下,随着归一化多普勒频移（frequency-normalized Doppler shift,FND）增大,新方法的信道估计均方差（mean square error,MSE）性能优于传统方法,如当SNR为20 dB,FND为0.1时,新方法较传统方法性能提升了4 dB,表明对信道时变性具有更优的鲁棒性;在相同的多普勒频移条件下,随着SNR增加,各方法的均方差均有所改善,新方法改善更明显,如当FND为0.2时,在信道估计均方差为0.06的条件下,新方法较传统方法获得了6 dB的信噪比增益,表明对抗信道噪声能力更强.      

Network of tRNA gene sequences

A network of 3719 tRNA gene sequences was constructed using simplest alignment. Its topology, degree distribution and clustering coefficient were studied. The behaviors of the network shift from fluctuated distribution to scale-free distribution when the similarity degree of the tRNA gene sequences increases. The tRNA gene sequences with the same anticodon identity are more self-organized than those with different anticodon identities and form local clusters in the network. Some vertices of the local cluster have a high connection with other local clusters, and the probable reason was given. Moreover, a network constructed by the same number of random tRNA sequences was used to make comparisons. The relationships between the properties of the tRNA similarity network and the characters of tRNA evolutionary history were discussed.     

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本文提出了一个支持向量机进行初始行程时间预测并结合卡尔曼滤波算法进行动态调整的快速公交车行程时间综合预测模型.以快速公交车运行的GPS数据为基础,对北京市朝阳区快速公交2号线进行行程时间预测案例研究.利用该模型对其早高峰和上午平峰的两个不同时段的公交行程时间分别进行预测和对比分析,并通过与单一的卡尔曼滤波方法所得的预测结果进行比较.结果表明,该模型应用于快速公交行程时间预测具有更好的适用性,并且预测平峰时段的精度要高于高峰时段.     

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提出考虑信息接收程度的Ｌogit型随机交通分配方法,来计算道路指引信息发布下路网交通的重新分配.出行者被划分成两类,一类根据信息的指引出行,另一类则以Logit型的随机方式进行路径选择;利用道路指引信息的市场占有率的概念,给出这两种出行行为共存下的网络加载算法,并运用逐次平均法实现了拥挤网络中的交通分配.数值试验结果表明,发布良好的道路指引信息有助于提高路网运行效率;对拥挤网络中的给定OD,存在一个最佳的占有率,并随着交通需求的增加而增加;在合理的占有率下,增加信息指引路段可有效降低路网运行时间.     

Infrared Image Reconstruction Based on Archimedes Spiral Measurement Matrix

It is a new research direction to realize infrared(IR) image reconstruction using compressed sensing(CS) theory. In the field of CS, the construction of measurement matrix is very principal. At present, the types of measurement matrices are mainly random and deterministic. The random measurement matrix can well satisfy the property of measurement matrix, but needs a large amount of storage space and has an inconvenient in hardware implementation. Therefore, a deterministic measurement matrix construction method is proposed for IR image reconstruction in this paper. Firstly, a series of points are collected on Archimedes spiral to construct a definite sequence; then the initial measurement matrix is constructed; finally, the deterministic measurement matrix is obtained according to the required sampling rate. Simulation results show that the IR image could be reconstructed by the measured values obtained through the proposed measurement matrix. Moreover, the proposed measurement matrix has better reconstruction performance compared with the Gaussian and Bernoulli random measurement matrices.     

山区高速公路长大纵坡车辙防治分析

长大纵坡的车辙是山区高速公路面临的严重问题之一,分析长大纵坡车辙的影响因素,并从材料、结构设计、施工和养护等方面提出防治措施,对于山区高速公路建设具有一定的指导意义。     

山区公路建设与生态环境保护探析

以某具体工程为例,针对山区公路建设给当地生态环境带来的影响,根据工程概况,结合环境保护原则及相关的技术标准,提出符合当地实际情况的环保措施,以降低山区公路建设对自然环境的不良影响。     

山区双车道公路货车碰撞预测的双变量冲突极值模型

为预测山区双车道公路货车与冲突车辆发生的碰撞，本文基于无人机视频，提取货车与交互车辆的高精度轨迹数据，选取适用于不同运行轨迹的交通冲突指标，结合极值理论，构建双变量冲突极值(BTCEV)模型，将后侵入时间(PET)与碰撞时间(TTC)纳入统一框架，实现山区双车道公路货车与冲突车辆的碰撞预测，并以云南省货车事故高发的山区双车道公路为例，验证 BTCEV模型的预测性能。研究表明：PET为0.382 s、TTC为4.471 s是山区双车道公路货车严重冲突的阈值；BTCEV 模型预测山区双车道公路货车年事故发生率为 5.84%，预测准确性高达 98.92%，较PET模型以及TTC模型分别提高了167.33%和10.80%；且相比于单变量模型，双变量模型所估计的置信区间更窄，预测精度更高。研究结果将山区双车道公路货车碰撞预测方法从单变量扩展到双变量，在山区货车交通安全分析方面有广阔的应用前景。     

神经干细胞经脑室移植后在损伤脊髓的早期动态分布

目的观察神经干细胞经脑室移植后在损伤脊髓的早期动态分布。方法取转录有绿色荧光蛋白(GFP)基因的孕16 d SD鼠胚脑海马组织,培养成神经干细胞球,注射到脊髓损伤鼠第四脑室,通过免疫染色和电镜,观察其在脊髓的存活、分化状况。结果移植细胞在脊髓表面形成细胞团,大多分布于损伤区头侧的腹、背侧两面。细胞团面积背侧小于腹侧;数目背侧多于腹侧,并于术后5 d达到高峰。这种分布和增殖形式见于损伤脊髓正常部分和无损伤脊髓(对照组)。1周时细胞侵入损伤区,分化并表达神经胶质纤维酸性蛋白(GFAP)。2~3周时与宿主细胞良好融合。结论通过脑脊液移植细胞对于损伤性脊髓进行干细胞移植是一种实用、有效的方法。     

公交司机排班问题的混合元启发算法研究

针对我国公交企业中司机在1 个工作日内驾驶同一辆车的“人车绑定”管理模式, 提出混合元启发算法求解司机排班问题.首先建立以车辆数为目标的车辆调度模型,获得仅 满足司机休息时间的非可行解;接着迭代地使用局部搜索算子、破坏重建扰动等方法对解进 行调整,使其满足司机工作时间和吃饭时间等约束,并尽可能地降低排班成本;在迭代搜索 过程中记录发现的可行排班链集合,迭代结束后构建集合覆盖问题(SCP)模型对其进行改 进,以获得最佳的司机排班方案.在13 条公交线路案例上进行测试,实验结果验证了本文算 法的有效性.     

山地城市应急救护中心选址研究

针对山地城市的路网结构特殊性,根据山地城市路网模型,提出了山地城市道路的连通率的计算方法,然后基于蒙特卡罗的模拟算法对震后路网可靠指标进行评估,并对山城重庆某局域路网进行了震后路网可靠性评估,分析评估结果为应急救护中心的选址提供依据.     

山区公路路线设计探析

山区公路路线的设计受到的制约因素比较多,应结合山区公路路线选线的准则和当地的具体条件,综合考虑平纵面设计、构造物的设计和环境保护等问题,设计出合理的山区公路路线。     

Improvement of oil/water selectivity by stearic acid modified expanded perlite for oil spill cleanup

Ecological disasters and economic losses resulting from oil spills have reminded us the necessity for finding an environmentally friendly, cost-effective, and available in large-scale materials to minimize the oil spill effects. The development of high oil/water selectivity mineral sorbent for the removal of oil from the water is of great interest for oil spill cleanup. In this work, highly oil/water selectivity sorbent of expanded perlite modified with stearic acid was prepared by solution-immersion processes, and the characterization of sorbent was analyzed by Fourier transformation infrared (FT-IR) spectroscopy technique. The optimized amount of loaded stearic acid (SA) on the expanded perlite surface was found to be 2.0%. Then the sorption characteristics of unmodified expanded perlite (UMEP) and stearic acid modified expanded perlite (SMEP) were tested. The effects of different particles size, oil/water volume ratio and sorption simulated condition were investigated. It was found that expanded perlite modified with stearic acid could adsorb selectively oil from water in oil/water system. The maximum oil/water sorption ratio of SMEP was 46, which was about 191 times that of UMEP (0.24) in studied experimental range. The FT-IR analysis demonstrated the presence of long-chain alkyl group in the SMEP samples, which played an important role in oil/water selectivity of SMEP samples. The equilibrium were tested with Langmuir and Freundlich isotherm models, and oil sorption process of SMEP showed good correlation with the Langmuir isotherm model.