STUDY ON SOLVENT EFFECT OF BATHOCHROMIC HETERO-CYCLIC AZO DYES

ＳＴＵＤＹＯＮＳＯＬＶＥＮＴＥＦＦＥＣＴＯＦＢＡＴＨＯＣＨＲＯＭＩＣＨＥＴＥＲＯ┐ＣＹＣＬＩＣＡＺＯＤＹＥＳ＊ＬｉｕＹａｎｇａｎｇ（刘燕刚）ＨａｎＬｉ（韩莉）ＨｕａｎｇＤｅｙｉｎ（黄德音）（Ｄｅｐｔ．ｏｆＡｐｐｌｉｅｄＣｈｅｍｉｓｔｒｙ，Ｓｈａｎｇｈ...     

用表达重组TSH受体的CHO细胞测定Graves'甲亢病人血清中的TSAb

用表达重组人ＴＳＨ受体的中国仓鼠卵细胞（Ｃｈｉｎｅｓｅｈａｍｓｔｅｒｏｖａｒｙｃｅｌ,ＣＨＯ）细胞代替传统的ＦＲＴＬ－５细胞测定Ｇｒａｖｅｓ甲亢病人的血清甲状腺刺激抗体（ＴｈｙｒｏｉｄＳｔｉｍｕｌａｔｉｎｇＡｎｔｉｂｏｄｙ,ＴＳＡｂ）水平,方法相对简单,特异性强,敏感性高,未治疗的Ｇｒａｖｅｓ病人９２．７％阳性,治疗后迅速下降,恢复期的阳性率为３５％,该方法对Ｇｒａｖｅｓ甲亢的诊断、治疗后效果的判断有重要意义。     

TERATOGENIC EFFECT OF ANTI-HBs-A CONCURRENT COHORT STUDY

ＴＥＲＡＴＯＧＥＮＩＣＥＦＦＥＣＴＯＦＡＮＴＩ－ＨＢｓ－ＡＣＯＮＣＵＲＲＥＮＴＣＯＨＯＲＴＳＴＵＤＹＹｕｅＹａｆｅｉ；ＺｈａｎｇＳｈｕｌｉｎ；ＭｅｎＢｏｙｕａｎ；ＬｉＹｉｆａｎｇ；ＣｈｅｎＴａｉｐｉｎｇ；ＷｕＬｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＯｂｓｔ...     

SIX-YEAR EFFICACY OF PLASMA-DERIVED HEPATITIS B VACCINE (ABSTRACT)

ＳＩＸ－ＹＥＡＲＥＦＦＩＣＡＣＹＯＦＰＬＡＳＭＡ－ＤＥＲＩＶＥＤＨＥＰＡＴＩＴＩＳＢＶＡＣＣＩＮＥ（ＡＢＳＴＲＡＣＴ）￥ＳｕｉＸｉｕｆｅｎ；ＸｕＨｕｉｗｅｎ，ＭｅｎＢｏｙｕａｎ；ＷａｎｇＸｕｅｌｉａｎｇ；ＬｉｕＰｅｎｇｂｏ（ＤｅｐａｒｔｍｅｎｔｏｆＥ...     

DETECTION OF E6 TRANSFORMATION CENE OF HUMAN PAPILLOMAVIRUS TYPE 16 IN ORAL CANCERS USING POLYMERASE CHAIN REACTIONS （ABSTRAC

ＤＥＴＥＣＴＩＯＮＯＦＥ６ＴＲＡＮＳＦＯＲＭＡＴＩＯＮＣＥＮＥＯＦＨＵＭＡＮＰＡＰＩＬＬＯＭＡＶＩＲＵＳＴＹＰＥ１６ＩＮＯＲＡＬＣＡＮＣＥＲＳＵＳＩＮＧＰＯＬＹＭＥＲＡＳＥＣＨＡＩＮＲＥＡＣＴＩＯＮＳ（ＡＢＳＴＲＡＣＴ）ＺｈａｎｇＴｏｎｇ；ＣｈｕＹｏ...     

74 CASES OF THE REDUCTION OF TRAUMATIC POSTERIOR DISLOCATION OF THE HIP WITH THE IMPROVED STIMSON＇S METHOD

７４ＣＡＳＥＳＯＦＴＨＥＲＥＤＵＣＴＩＯＮＯＦＴＲＡＵＭＡＴＩＣＰＯＳＴＥＲＩＯＲＤＩＳＬＯＣＡＴＩＯＮＯＦＴＨＥＨＩＰＷＩＴＨＴＨＥＩＭＰＲＯＶＥＤＳＴＩＭＳＯＮ＇ＳＭＥＴＨＯＤＳｈｉＭｉｎｇｑｉ（史明起）；ＬｉｕＳｈｉｆｕ（刘世甫）（Ｄｅｐａｒｔ...     

MULTIPLE TRANS-ACTING FACTORS MEDIATE THE INDUCTION OF C-FOS IN VASCULAR SMO

ＭＵＬＴＩＰＬＥＴＲＡＮＳ－ＡＣＴＩＮＧＦＡＣＴＯＲＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．Ｎａｆｔｉｌａｎ（Ｄｅ...     

THREE－YEAR FOLLOW－UP AFTER A SINGLE BOOSTER DOSE OF VACCINE IN THE NONRESPONDERS AND HYPORESPONDERS TO HEPATITIS B VACCINE

ＴＨＲＥＥ－ＹＥＡＲＦＯＬＬＯＷ－ＵＰＡＦＴＥＲＡＳＩＮＧＬＥＢＯＯＳＴＥＲＤＯＳＥＯＦＶＡＣＣＩＮＥＩＮＴＨＥＮＯＮＲＥＳＰＯＮＤＥＲＳＡＮＤＨＹＰＯＲＥＳＰＯＮＤＥＲＳＴＯＨＥＰＡＴＩＴＩＳＢＶＡＣＣＩＮＥ（王学良）（徐慧文）（隋秀芬）（门伯媛）...     

PRODUCTION OF PARATHYROID HORMONE－RELATED PROTEIN （PTHrP） BY A HUMAN HEPATOMA CELL LINE

ＰＲＯＤＵＣＴＩＯＮＯＦＰＡＲＡＴＨＹＲＯＩＤＨＯＲＭＯＮＥ－ＲＥＬＡＴＥＤＰＲＯＴＥＩＮ（ＰＴＨｒＰ）ＢＹＡＨＵＭＡＮＨＥＰＡＴＯＭＡＣＥＬＬＬＩＮＥＬｉＨｕｓｏｎｇ；ＰＫ．Ｓｅｉｔｚ，Ｐ．Ｓｅｌｖａｎａｙａｇａｍ，Ｓ．Ｒａｊａｒａｍａｎ，Ｃ．Ｃｏ...     

THE STUDY OF RED BLOOD CELL IMMUNE FUNCTIONS AND THEIR RELATIONSHIP WITH T LYMPHOCYTE SUBSETS AND FREE RADICAL IN CHILDREN WI

ＴＨＥＳＴＵＤＹＯＦＲＥＤＢＬＯＯＤＣＥＬＬＩＭＭＵＮＥＦＵＮＣＴＩＯＮＳＡＮＤＴＨＥＩＲＲＥＬＡＴＩＯＮＳＨＩＰＷＩＴＨＴＬＹＭＰＨＯＣＹＴＥＳＵＢＳＥＴＳＡＮＤＦＲＥＥＲＡＤＩＣＡＬＩＮＣＨＩＬＤＲＥＮＷＩＴＨＭＹＯＣＡＲＤＩＴＩＳ（ＡＢＳＴＲＡ...     

雌、孕激素受体和rasP~(21)蛋白的表达在乳腺癌预后中的意义

用酶联亲合组化法和ABC免疫组化法对93例乳腺癌石蜡标本中雌激素受体(ER)、孕激素受体(PR)和ras P~(21)蛋白进行了检测和对比研究。经果表明:ER、PR阳性率分别为64.52％和60.22％,经随访5年累积无瘤生存率阳性组高于阴性组(P<0.05);P~(21)蛋白的阳性表达率为52.69％,多出现在分化差的Ⅱ、Ⅲ级具有浸润性的乳腺癌组织中,随乳腺癌组织学分级的增加而增强,与ER水平呈负相关,5年累积无瘤生存率阴性组高于阳性组(P<0.05)。认为P~(21)蛋白表达的强弱同ER、PR一样反映了乳腺癌的生物学特性及预后。     

联合应用MG_7－Ag和AgNOR检测胃癌及萎缩性胃炎的研究

用ＭＧ_７－Ａｇ和ＡｇＮＯＲ法对３０例胃癌、６４例慢性萎缩性胃炎（ＣＡＧ）、２０例正常胃粘膜研究。发现胃癌ＭＧ_７－Ａｇ强阳性高于ＣＡＧ（Ｐ＜０．００５）；中、重度ＣＡＧ或伴ＩＭ者高于轻度或局限胃窦ＣＡＧ（Ｐ＜０．０５）。以细胞核内ＡｇＮＯＲ计数明显增多（＞９．２６）或大小不等，呈团块状分布为阳性指标，则胃癌检出率高于ＣＡＧ（Ｐ＜０．００５），中、重度ＣＡＧ高于轻度（Ｐ＜０．０５）。ＭＧ_７－Ａｇ和ＡｇＮＯＲ联用，胃癌检出率可达９０％，高于单用任何一项者（Ｐ＜０．０２５），而假阳性检出与单项应用比Ｐ＞０．１。可为临床区分良恶性胃病提供有力的客观证据。     

Induction of animal model of Graves' disease in BALB/c mice

Objective To construct an animal model of Graves' disease (GD) by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex (MHC) class Ⅱ molecules and human thyrotropin receptor (TSHR) molecules. Methods BALB/c mice in experimental group (H-2d) were immunized with hM12 cells Intraper-itoncally every 2 weeks for six times, while mice in control group were immunized with M12 cells. Five weeks later, the thyroids were histologically examined, and serum samples were tested for thyroid-stimulating antibodies (TSAb) and thyroid hormone levels. Results One BALB/c mouse in experimental group developed Graves'-like disease. Total T4 and T3 levels in this mouse were above the upper limit of normal, TSAb activity was displayed in its serum. The thyroid histologically showed the features of thyroid hyperactivity including thyrocyte hypercellularity and colloid absorption.None of control mice developed Graves'-like disease. Conclusion An animal model with some characteristics of human Graves' disease was successfully induced and the model will facilitate studies aimed directly at understanding the patho-genesis of autoimmunity in Graves' disease.     

秦巴山区孕妇人巨细胞病毒宫内感染与UL54、UL97基因突变的关系

目的探讨秦巴山区孕妇妊娠期人巨细胞病毒(HCMV)宫内感染与HCMV UL54、UL97基因突变的关系。方法采用PCR方法检测228对孕妇血清和新生儿脐血血清中的HCMV DNA,对HCMV DNA阳性者采用限制性片段长度多态性(PCR-RFLP)方法检测HCMV UL54、UL97基因501、594及595密码子是否发生突变。结果①228例孕妇血清中HCMV DNA阳性者19例,阳性率为8.33%;新生儿脐血血清中HCMV DNA阳性8例,阳性率为3.51%。在19例HCMV DNA阳性孕妇中,其配对脐血阳性7例,HCMV宫内传播率为36.84%。②HCMVDNA阳性孕妇血清及其配对脐血阳性的标本中均未发现HCMV UL54、UL97基因501、594及595密码子发生突变。结论秦巴山区HCMV宫内感染的发生率较高,其发生与HCMV UL54、UL97基因501、594及595密码子突变无相关性,但不排除存在其他位点突变或其他形式基因异常导致病毒致病性改变的可能性。     

不孕不育与支原体感染和抗精子抗体的关系

目的　探讨不孕不育与支原体感染和抗精子抗体 (ASA)之间的关系。方法　采用培养法分别对 2 4 8例不孕妇女(观察组 )和 16 7例不育男性患者 (观察组 )进行解脲支原体 (UU)和人型支原体 (MH)检测 ;采用ELISA法检测血清抗精子抗体ASA IgM和ASA IgG。同时分别选择 6 9例正常妇女和 4 3例正常男性作为对照。结果　不孕症观察组及不育症观察组UU、MH阳性检出率均明显高于对照组 (P <0 .0 5 )。不孕和不育观察组ASA阳性检出率 (2 8.6 3% ,2 0 .96 % )均显著高于对照组 (7.2 1% ,6 .98% ,P <0 .0 5 )。支原体感染阳性组ASA阳性率明显高于支原体感染阴性组(P <0 .0 2 5 )。结论　女性不孕和男性不育均与支原体感染及免疫因素有关 ,并且ASA与支原体感染有关     

EXPRESSION OF MATRIX METALLOPROTEINASE-9 IN HUMAN ABDOMINAL AORTIC ANEURYSMAL TISSUES

Objective To study the effects of MMP-9 （Matrix Metalloproteinase-9, MMP-9） in the pathogenesis of abdominal aortic aneurysms （AAAs） by localizing the expression of MMP-9 in the aneurysmal tissues. Methods By , the frozen sections （5μm） with aneurysmal tissues （n = 10） were incubated with MMP-9 antibody-added agents, then the sections were stained and observed under the microscope to localize the expression of MMP-9, which displayed a brown precipitate within the arterial walls. The normal arterial wall tissues（n= 10） and the diseased arterial wall tissues from the arterial occlusive diseases （AODs） （n= 15） were also immunized exactly the same way as control. Results A quantity of positive granules which appeared within the aortic media showed the strong expression of MMP-9 in the AAAs, with the positive rate reaching 95 % （19/20）, while no expression of MMP- 9 was observed in the normal artery. However, the scattered distributed positive granules were seen within the arterial wall of some cases of the AOI）s, implying the weak positive expression of MMP-9 in this disease with the positive rate of 26.7% （4/15）. There was a significant difference of the expression of MMP-9 within the arterial wall between the AAAs and AODs（P〈0.01 ）. Conclusion High expression of MMP-9 within the aortic media faciliatates the degradation of collagen and elustin fibres and subsequent dilation of the aortic artery , thus playing an important role in the pathogenesis of AAAs. To refrain MMP-9 from enhanced expressing within the aortic wall is of clinical significance in the prevention and treatment of AAAs.     

THE DETECTION OF HPV16E6，E7 GENES IN TISSUES AND THEIR ANTIBODIES IN THE SERA OF PATIENTS WITH CERVICAL CARCINOMA

ＴＨＥＤＥＴＥＣＴＩＯＮＯＦＨＰＶ１６Ｅ６，Ｅ７ＧＥＮＥＳＩＮＴＩＳＳＵＥＳ　ＡＮＤＴＨＥＩＲ　ＡＮＴＩＢＯＤＩＥＳＩＮＴＨＥＳＥＲＡＯＦＰＡＴＩＥＮＴＳＷＩＴＨ　ＣＥＲＶＩＣＡＬＣＡＲＣＩＮＯＭＡＬｉｕＴｉａｎｊｕ；ＬｉｕＨｕａ；，ＳｕｎＹｉ；，Ｓ...     

胃癌中EGFR和C-erbB-2基因表达的临床研究

应用免疫组化技术（ABC法），对94例胃癌及癌旁组织中表皮生长因子受体（EGFR）和C-erbB－2的表达进行了研究，结果显示：①EGFR和C－erbR－2在正常胃粘膜中均无表达，而在胃癌中的表达率分别为40．4％和46．8％，癌旁组织及新生血管中EGFR有阳性表达，C－erbB－2表达只限于癌灶，癌旁组织均为阴性；②EGFR和C－erbB－2的表达与患者的性别、年龄和肿瘤大小无关（P＞0．05），而与肿瘤的生长方式和浆膜层受浸与否关系密切（P＜0．05）；③肿瘤的分化程度、有无淋巴结转移和临床分期与EGFR的表达有相关性（P＜0．01），而与C－erbB－2的表达不相关（P＞0．05）；④EGFR表达与生存期密切相关（P＜0．01），EGFR阳性者预后差，阴性者预后好，而C－erbB－2表达与生存期不相关（P＞0．05）；⑤EGFR和C－erbB－2在胃癌中的表达无明显关系（P＞0．05）。结果表明：①EGFR表达与胃癌的恶性程度有明显的关系；②C－erbB－2表达是胃癌发生的晚期事件，是恶性细胞的标志；③EGFR可作为预测胃癌的预后和指导治疗的指标之一。④EGFR和C－erbB－2在胃癌中的表达相互独立。     

阻断5-HT1B受体对正常和帕金森病大鼠底丘脑核神经元活动的不同作用

目的观察体循环给予5-HT1B受体拮抗剂SB224289后,对正常大鼠和帕金森病(Parkinsons disease,PD)模型大鼠底丘脑核(subthalamic nucleus,STN)神经元电活动的影响。方法采用在体细胞外记录的方法,研究正常和PD大鼠STN神经元电活动的变化;比较阻断5-HT1B受体后两组大鼠STN神经元电活动的改变。结果①PD组大鼠STN神经元的平均放电频率[(7.87±1.37)Hz]明显高于正常组大鼠[(6.59±1.44)Hz,P<0.001]。两组大鼠STN神经元均表现为规则、不规则和暴发式放电3种形式。正常组大鼠具有规则、不规则和暴发式放电的神经元比例分别为33.33%、54.17%和12.50%;PD组分别为31.25%、35.42%和33.33%,PD组大鼠具有暴发式放电的神经元的百分比显著高于正常组(P<0.01)。②阻断5-HT1B受体后,正常大鼠STN神经元平均放电频率明显增高(P<0.01),具有暴发式放电的神经元数量显著增多(P<0.05)。而PD组大鼠阻断5-HT1B受体后STN神经元的放电频率和放电形式均无明显变化。结论 PD大鼠STN神经元呈过度兴奋状态;阻断5-HT1B受体可兴奋正常大鼠的STN神经元,而不影响PD大鼠的神经元活动。     

急性白血病多药耐药蛋白的异常表达及其与临床疗效和预后的关系

目的研究多药耐药蛋白P-糖蛋白(PGP)、多药耐药相关蛋白1(MRP1)、谷胱甘肽-硫-转移酶π(GST-π)和拓扑异构酶Ⅱα(TOPOⅡα)在初发急性白血病(AL)患者中的表达,并探讨其与疗效及预后之间的关系。方法采用免疫组化SABC法检测了PGP、MRP1、GST-π、TOPOⅡα在30例AL患者白血病细胞中的表达情况。结果PGP、MRP1、GST-π、TOPOⅡα在初发AL中表达的阳性率分别为23.33%、36.6%、60%和43.33%;PGP、MRP1阳性组的完全缓解(CR)率均明显低于阴性组(P<0.01);TOPOⅡα阳性组的CR率为92.31%,高于阴性组的58.82%(P<0.05);GST-π阳性组的CR率与阴性组的CR率之间无统计学意义(P>0.05)。结论单一PGP、MRP1的表达升高及TOPOⅡα的表达降低与AL的治疗反应差、预后不良有明显的相关性;联合多个多药耐药蛋白能够更准确地预测AL的耐药和预后。