A STUDY ON THE ALTERATION OF FREE RADICALS、PROSTAGLANDINS AND THEIR RELATIONSHIP IN CHILDREN EITH ACUTE GLOMERULONEPHRITIS

ＡＳＴＵＤＹＯＮＴＨＥＡＬＴＥＲＡＴＩＯＮＯＦＦＲＥＥＲＡＤＩＣＡＬＳ、ＰＲＯＳＴＡＧＬＡＮＤＩＮＳＡＮＤＴＨＥＩＲＲＥＬＡＴＩＯＮＳＨＩＰＩＮＣＨＩＬＤＲＥＮＥＩＴＨＡＣＵＴＥ　ＧＬＯＭＥＲＵＬＯＮＥＰＨＲＩＴＩＳＹａｎＸｉａｏｃａｉ；，ＷｅｉＪｕ...     

A COMPARATIVE STUDY ON THERAPEUTIC RESPONSE AND SURVIVAL TIME OF PATIENTS WITH MULTIPLE MYELOMA TREATED WITH MODIFIED VMCP RE

ＡＣＯＭＰＡＲＡＴＩＶＥＳＴＵＤＹＯＮＴＨＥＲＡＰＥＵＴＩＣＲＥＳＰＯＮＳＥＡＮＤＳＵＲＶＩＶＡＬＴＩＭＥＯＦＰＡＴＩＥＮＴＳＷＩＴＨ　ＭＵＬＴＩＰＬＥＭＹＥＬＯＭＡＴＲＥＡＴＥＤ　ＷＩＴＨ　ＭＯＤＩＦＩＥＤＶＭＣＰＲＥＧＩＭＥＮＣｈｅｎＸｕｅｍｉｎ...     

COMPARATIVE STUDIES ON THE CORNEAL STRUCTURE OF MAMMAL ANIMALS：I．ULTRASTRUCTURE OF THE CORNEAL SUBSTANTIA PROPRIA IN GUINEA P

ＣＯＭＰＡＲＡＴＩＶＥ　ＳＴＵＤＩＥＳ　ＯＮ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＴＲＵＣＴＵＲＥ　ＯＦ　ＭＡＭＭＡＬ　ＡＮＩＭＡＬＳ：Ｉ．ＵＬＴＲＡＳＴＲＵＣＴＵＲＥ　ＯＦ　ＴＨＥ　ＣＯＲＮＥＡＬ　ＳＵＢＳＴＡＮＴＩＡ　ＰＲＯＰＲＩＡ　ＩＮ　ＧＵＩＮＥＡ　Ｐ...     

REGULATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASEPKR AND2＇5＇ OLIGOADENYLATE SYNTHETASE BY A CATALYTICALLY INACTIVE PKR M

ＲＥＧＵＬＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＡＮＤ２＇５＇ＯＬＩＧＯＡＤＥＮＹＬＡＴＥＳＹＮＴＨＥＴＡＳＥＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＰＫＲ　ＭＵＴＡＮＴＴＨＲＯＵＧＨ　Ｃ...     

ACOUSTIC SYSTEMS WITH MULTIPLE INCOHERENT SOURCES AND ITS APPLICATION IN STUDYING NOISES RADIATED FROM VEHICLE ENGINE

ＡＣＯＵＳＴＩＣＳＹＳＴＥＭＳＷＩＴＨＭＵＬＴＩＰＬＥＩＮＣＯＨＥＲＥＮＴＳＯＵＲＣＥＳＡＮＤＩＴＳＡＰＰＬＩＣＡＴＩＯＮＩＮＳＴＵＤＹＩＮＧＮＯＩＳＥＳＲＡＤＩＡＴＥＤＦＲＯＭＶＥＨＩＣＬＥＥＮＧＩＮＥＪｉａｎｇＷｅｉｋａｎｇ（蒋伟康）（Ｓｃｈｏｏ...     

VALUE OF MOTOR EVOKED POTENTIALS BY MAGNETIC STIMULATION IN DIAGNOSIS OF LUMBOSACRAL RADICULOPATHY

ＶＡＬＵＥＯＦＭＯＴＯＲＥＶＯＫＥＤＰＯＴＥＮＴＩＡＬＳＢＹＭＡＧＮＥＴＩＣＳＴＩＭＵＬＡＴＩＯＮＩＮＤＩＡＧＮＯＳＩＳＯＦＬＵＭＢＯＳＡＣＲＡＬＲＡＤＩＣＵＬＯＰＡＴＨＹＹａｎｇＺｈｅ；ＣｈｅｎＪｕｎｃｈａｎｇ；ＺｈａｏＬｏｎｇｚｈｕＷａｎｇＫｕｎ...     

LOSS OF NUCLEUS BASALIS MAGNOCELLULARIS，BUT NOT SEPTAL，CHOLINERGIC NEURONS CORRELATES WITH PASSIVE AVOIDANCE IMPAIRMENT IN RA

ＬＯＳＳＯＦＮＵＣＬＥＵＳＢＡＳＡＬＩＳＭＡＧＮＯＣＥＬＬＵＬＡＲＩＳ，ＢＵＴＮＯＴＳＥＰＴＡＬ，ＣＨＯＬＩＮＥＲＧＩＣＮＥＵＲＯＮＳ　ＣＯＲＲＥＬＡＴＥＳＷＩＴＨＰＡＳＳＩＶＥＡＶＯＩＤＡＮＣＥＩＭＰＡＩＲＭＥＮＴＩＮＲＡＴＳＴＲＥＡＴＥＤ　ＷＩＴ...     

DIETARY MAGNESIUM DEFICIENCY INCREASES LEVELS AND FUNCTION OF Giα IN THE RAT HEART AFTER MYOCARDIAL INFARCTION

ＤＩＥＴＡＲＹＭＡＧＮＥＳＩＵＭＤＥＦＩＣＩＥＮＣＹＩＮＣＲＥＡＳＥＳＬＥＶＥＬＳＡＮＤＦＵＮＣＴＩＯＮＯＦＧｉαＩＮＴＨＥＲＡＴＨＥＡＲＴＡＦＴＥＲＭＹＯＣＡＲＤＩＡＬＩＮＦＡＲＣＴＩＯＮＳｈｉＢｉｎｇ；Ｊ．Ｅ．Ｈｅａｖｎｅｒ，Ｌ．Ｍ．Ｂｏｙｌａｎ...     

THE DISTRIBUTION OF ATRIONATRIURETIC POLYPEPTIDE （ANP）－LIKE SUBSTANCE IN THE HEART OF THE HUMAN FETUS AND RAT－AN IMMUNOHISTOC

ＴＨＥＤＩＳＴＲＩＢＵＴＩＯＮＯＦＡＴＲＩＯＮＡＴＲＩＵＲＥＴＩＣＰＯＬＹＰＥＰＴＩＤＥ（ＡＮＰ）－ＬＩＫＥＳＵＢＳＴＡＮＣＥＩＮＴＨＥＨＥＡＲＴＯＦＴＨＥＨＵＭＡＮＦＥＴＵＳＡＮＤＲＡＴ－ＡＮＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＳＴＵＤＹＬｅｉＴ...     

LEISHMANIA PROMASTIGOTES EVADE INTERLEUKIN 12 INDUCTION BY MACROPHAGES AND STIMULATE A BROAD RANGE OF CYTOKINES FROM CD_（4）~（＋

ＬＥＩＳＨＭＡＮＩＡＰＲＯＭＡＳＴＩＧＯＴＥＳＥＶＡＤＥＩＮＴＥＲＬＥＵＫＩＮ１２ＩＮＤＵＣＴＩＯＮＢＹＭＡＣＲＯＰＨＡＧＥＳＡＮＤＳＴＩＭＵＬＡＴＥＡＢＲＯＡＤ　ＲＡＮＧＥＯＦＣＹＴＯＫＩＮＥＳＦＲＯＭ　ＣＤ_（４）~（＋）ＴＣＥＬＬＳＤＵＲＩＮＧＩ...     

霜天蛾cDNA表达文库的构建和初步鉴定

目的构建霜天蛾cDNA表达文库,为进一步筛选霜天蛾主要变应原、制备重组变应原奠定基础。方法利用Trizol法抽提霜天蛾RNA,经过Oligo(dT)纤维素柱纯化后得到mRNA,反转录合成dscDNA,连接EcoRⅠ接头,末端磷酸化,XhoⅠ消化,将双末端黏性化的双链dscDNA经过凝胶柱层析,收集大于400bp的cDNA片段,加工后与UniZAPXRVector连接,经过体外包装,感染宿主菌,构建cDNA文库,检测文库容量和重组效率,并采用PCR方法对插入的cDNA片段大小进行分析。结果成功构建了一个含有5×105重组子的霜天蛾表达文库,重组率为67%,平均插入片段长度约为1.49kb。结论所建文库容量及插入片段大小合适,适用于筛选目的cDNA克隆。     

ADENO-ASSOCIATED VIRUS INTRODUCED INTEGRATION AND EXPRESSION OF FOREIGN GENES IN PC12 CELLS

The ability to induce the expression of targetgenes in neurons is a goal of molecular neurobiolo-gy up to now. A variety of means including lipo-somes and recombinant retrovirus vectors and re-combinant adenovirus vectors have been used to ac-coplish this goal. Each method has some specificdisadvantages['J. None can provide reliable andcontinuouk gene expression in neurons of the cen-tral nervous system.Vectors derived from adeno--associaed virus(AAV ) have the potential to stably transducem…     

一种通用型重组2型腺伴随病毒载体的构建及外源基因表达的研究

以野生型2型人腺伴随病毒（AAV－2）基因组载体pSSV9－int-为基础，构建了一种携带和表达真核基因的基础AAV载体pSSV9／MulV－neosv，表达了neo基因，并采用该载体组建了携带人白细胞介素-2cDNA的重组AAV载体pSSV9／MulV－neosv－IL－2，转染A549细胞后表达了人白细胞介素-2基因。为应用腺伴随病毒作基因治疗研究提供了实验基础。     

葎草花粉cDNA表达文库的构建和初步鉴定

目的构建葎草花粉cDNA表达文库,为进一步筛选葎草花粉的主要致敏蛋白组分、制备重组变应原奠定基础。方法采集新鲜葎草花粉,过筛后液氮保存,利用Trizol法抽提葎草花粉总RNA,纯化后PCR法反转录合成cDNA。经SfiⅠ酶切,过凝胶柱层析,去除小于400 bp的片断,收集大于400 bp的cDNA片段,加工后与λTripIEx2连接,文库体外包装,取出一小部分感染宿主菌XL1-Blue MRF,检测文库容量;PCR分析插入的cDNA片段的大小及多样性。结果成功构建一个含有5×105重组子的葎草花粉表达文库,重组率为90%,平均插入片段长度约为1.02 kb。结论所建文库容量及插入片段大小合适,适用于筛选克隆的目的cDNA。     

A novel mutation-L539fs/47 of hERG in a Chinese long QT syndrome family

Objective To identify the mutation of human ether-a-go-go-related gene (hERG) and analyze the clinical characteristics of a Chinese family with long ST syndrome (LQTS). Methods The electrocardiogram and DNA samples were obtained from a Chinese LQTS family of 26 members. Genotype was performed with polymorphic short tandem repeat (STR) markers at the known LQT1, LQT2, and LQT3 loci. SSCP analysis was used to find aberrant conformers. hERG mutation was confirmed by cloning and sequencing. Results Three gene carriers were linked to chromosome 7q35-36, where the potassium channel gene hERG was encoded. A 19-base pair deletion was identified. The mutation was located at nucleotide position 1 619-1 637 between transmembrane domains S4 and S5. Furthermore, A1692G polymorphism was found both in the normal control and patients. Conclusion A novel 19 bp deletion mutation of hERG is identified in a Chinese family. All gene carriers are demonstrated to be typical LQT2 ECG phenotype.     

EFFECT OF MIFEPRISTONE (RU486)ON MARKERS OF ENDOMETRIAL RECEPTIVITY

Mifepristone (RU4 86 )hasbeenusedinclinicasaneffectiveemergencycontraception (EC)meth od .And ,areductionfrom 6 0 0mgto 10mgdidnotinfluenceefficacy[1] .However ,themechanismisnotclear .Inpractice ,unprotectedsexualintercoursecanoccuratanytimeinamenstrualcycle .AnidealECwouldbeonewhichcouldblockovulationandimplantation .Thus ,theeffectofEContherecep tivityoftheendometriumiscriticalforitseffec tiveness .Implantationofthehumanblastocystintheen dometriumisconsideredtobeinitiatedbyadia loguebet…     

增强型绿色荧光蛋白标记的Hela细胞株的建立

目的构建增强型绿色荧光蛋白(EGFP)标记的Hela细胞系。方法PCR和DNA测序鉴定pEGFP-C1真核表达质粒,采用Qiagen tip 500进行pEGFP-C1真核表达质粒DNA的提取和纯化;cellfectin转染Hela细胞,G418筛选,有限稀释法单克隆培养,荧光显微镜进行荧光检测EGFP的表达,采用激光共聚焦显微镜观测器检测荧光特性。结果PCR和DNA测序证实pEGFP质粒结构正确;在倒置荧光显微镜下,转染24h后,部分Hela细胞可见绿色荧光,转染72h时,大约80%Hela细胞内均可见绿色荧光。加入含G418的选择性培养基进行选择性培养,选择荧光较强的Hela细胞经有限稀释,持续筛选及克隆化培养,获得稳定表达绿色荧光的Hela细胞克隆,扩大培养并传至10代以上,将此细胞株命名为Hela-EGFP。激光共聚焦显微镜观察发现:在蓝光(-395nm)激发时,Hela-EGFP细胞绿色荧光激发波长为395nm,最大发射峰为509nm。结论Hela-EGFP细胞株具备稳定表达EGFP的能力,为实时可视化进行宫颈癌侵袭转移机制的研究奠定了基础。     

重组小鼠血管抑素基因在胆囊癌细胞中的表达及对血管内皮细胞增殖的抑制作用

目的　研究重组小鼠血管抑素基因真核表达质粒转染的胆囊癌细胞能否表达具有抑制血管内皮细胞生长活性的血管抑素蛋白。方法　应用脂质体LipofectAMINE 2 0 0 0将重组小鼠血管抑素基因的真核表达质粒 pcDNA3.1(+) angiostatin转染胆囊癌细胞株GBC SD ,G4 18抗性筛选 ;以Westernblot检测血管抑素的表达 ,以血管内皮细胞增殖分析检测其活性。结果　经Westernblot检测 ,得到高表达血管抑素的胆囊癌细胞克隆 ,其培养上清能有效抑制bFGF刺激的血管内皮细胞增殖 (P <0 .0 5 )。结论　小鼠血管抑素基因真核表达质粒 pcDNA3.1(+) angiostatin转导的胆囊癌细胞能够表达具有生物学活性的血管抑素蛋白 ,在胆囊癌的血管抑制基因治疗中有潜在临床应用价值     

反义HSP70真核表达载体的构建和在人喉癌细胞的表达

目的　进行促凋亡治疗喉癌实验 ,构建和鉴定携带反义HSP70 (heatshockprotein 70 )基因的真核表达载体。方法　将HSP70cDNA反向克隆入 pcDNA 3.1,构建CMV启动子控制的真核表达载体 pcDNA AHSP70 ,用酶切鉴定结果 ;应用该载体转染人喉表皮样癌细胞Hep 2 ,G4 18筛选阳性克隆 ;westernblot和免疫组化检测转染前后瘤细胞的HSP70的表达。检测转染前后瘤细胞的生物学性状。结果　获得 pcDNA AHSP70真核表达载体 ,HSP70反义RNA阻断了Hep 2细胞的HSP70的表达 ,经westernblotting和免疫组化证实 ,实验组瘤细胞不能表达或低表达HSP70 ,而对照和空载体组高表达HSP70。转染反义HSP70的真核表达载体的Hep 2细胞比空载体组和对照组的细胞生长缓慢 ,细胞周期可见实验组出现亚二倍凋亡峰 ,而空载体组没有。结论　本研究成功构建了反义HSP70真核表达载体并在人喉癌细胞得以表达。