Electrogenerated chemiluminescence sensor for the determination of metoclopramide using ordered mesoporous carbon for immobilizing tris(2, 2'-bipyridyl) ruthenium

A novel electrogenerated chemiluminescence (ECL) sensor for the determination of metoclopramide was developed by employing ruthenium complex as an ECL signal producer and an ordered mesoporous carbon (OMC) material as modified material. The ECL sensor was fabricated by adsorption ruthenium complex into a mixture of OMC and Nafion, which showed good electrochemical and ECL behaviors. It was found that the ECL intensity of the sensor fabricated was greatly enhanced in the presence of metoclopramide. Based on this finding, a highly sensitive and reproducible ECL method was developed for the determination of metoclopramide. The result showed that the ECL intensity was linear with the concentration of metoclopramide in the range from 1.0×10-10 to 5.0×10-7M and the detection limit was 3×10-11M. The ECL sensor exhibited a long-term stability and a fine reproducibility with relative standard deviation of 1.0 % for 1.0×10-10M metoclopramide in 18 continuous determinations. The developed method has been applied to the determination of metoclopramide in tablet samples with satisfactory results.     

Capillary electrophoresis coupled with electrochemiluminescence for determination of cloperastine hydrochloride

Objective To investigate the electrochemiluminescence (ECL) behavior of cloperustine hydrochloride.Methods ECL intensity of tris (2,2'-bipyridyl) rutheniumo(Ⅱ) was enhanced, the method for the determination of dupernstine hydrochloride was established using capillary electrophoresis (CE) coupled with electrochemilumolinescence (ECL) detection. Results Under the optimum conditions, ECL intensity varied linearly with cloperastine hydrochloride concentration from 7.0×10-6 g/mL to 1.0×10-4 g/mL. The detection limit (S/N=3) was 8.05×10-7g/mL. he relative standard deviation of the ECL intensity and the migration time for 11 consecutive injections of 1.0 ×10-5g/mL cloperastine hydrochloride was 2.9% and 1.5%, respectively. This method was successfully applied to eloperastine hydrochloride tablet determination. Conclusion The method has been established, validated and applied for determination of cloperastine hydrochloride.     

CE-ECL法同时测定复方磷酸可待因中三种成分的方法学研究

目的 建立毛细管电泳-电化学发光法分离并检测复方磷酸可待因中三种成分,磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱含量的新方法.方法 基于碱性介质中,复方磷酸可待因溶液中三种主要成分对联吡啶钌在铂电极上的电化学发光信号的增敏作用,且增敏强度和药物浓度成线性关系,与毛细管电泳联用,建立了毛细管电泳-电化学发光法分离并检测其含量的新方法.结果 在优化的实验条件下,确定三种药物对照品的线性范围、线性方程和检出限.通过对浓度为1.0×10~(-5)kg/L的磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱对照品进行12次平行测定,其ECL发光强度的RSD值分别为2.89%,3.76%,3.32%.结论 该方法可用于复方磷酸可待因口服溶液中三种主要成分的含量测定,待测样品中的基质不干扰测定,方法的回收率分别为100.4%,101.9%,99.8%(n=5).     

Design of DC Partial Discharge Detecting System for Electrical Equipment

A balancing method was used to build a DC partial discharge (PD) testing circuit for electrical equipment, and a narrow-band detection system was designed using detection resistance and a filter. After signal accessed a high-speed digital acquisition (DAQ) card, the system was triggered to extract a single partial discharge (PD) signal. To eliminate the interference pulses caused by power supply ripple, etc., the time domain and frequency domain features of pulses were extracted. Based on the features, cluster analysis was used to exclude interference pulses. Two-dimensional and three-dimensional histograms were obtained by use of the Δt method. Then, 22 discharge statistical operators were calculated for the two-dimensional charts. Lastly, the defective system is capable of PD detection in electrical equipment.     

KCNE2蛋白在自发性高血压大鼠心脏组织中的分布和表达

目的研究KCNE2蛋白在自发性高血压大鼠(SHR)心脏不同部位的分布和表达差异,及鼠龄对其表达水平的影响。方法提取幼龄和高龄SHR心肌不同部位(心室、室隔膜和心房)组织的膜蛋白,利用Western印迹技术和图象分析方法,比较KCNE2蛋白在样品之间免疫强度差异。结果SHR心肌不同部位组织中均有KCNE2蛋白的分布;同龄大鼠心肌不同部位组织中KCNE2蛋白的表达均无显著性差异;但在高龄大鼠的右心室和室隔膜组织中,KCNE2蛋白的表达显著高于其在幼龄大鼠相同组织中的表达。结论KCNE2蛋白在SHR心脏不同部位均有分布;在高龄大鼠右心室和室隔膜组织中,KCNE2蛋白的表达水平均高于其在幼龄大鼠同一组织中的表达。     

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故障检测与隔离是保证组合导航系统容错性能的重要措施。本文针对车辆导航系统的可靠及安全性需求,分析了组合导航系统的传感器故障特性,建立了包含三个数据通路的车辆组合导航系统容错设计结构,提出了一种基于主成分分析的故障检测与隔离算法。该算法利用数据的相关关系,采用正常条件下的导航历史数据建立统计PCA模型,通过一定的统计量控制限检验新的导航数据样本相对于模型的背离程度实现故障检测,并根据不同数据通路的故障检测结果动态调整导航子系统的有效性因数,从而隔离故障造成的影响。仿真结果表明,该算法实现简单,能够有效实现对组合导航系统故障的检测与隔离。     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection

A rapid method has been developed based on the sample preparation procedure named as QuEChERS （Quick, Easy, Cheap, Effective, Rugged and Safe）, combined with reversed-phase high performance liquid chromatography with fluorescence detector and C18 column after precolumn derivatization using o-phthalaldehyde and 2-mercaptoethanol to determine dopamine in porcine muscle. Methanol and deionized water （0.1% acetic acid, v/v） with a ratio of 60：40 was used as mobile phase. The flow rate was 0.8 mL/min and dopamine was eluted within 15 min. The linearity range was 0.003-8 μg/mL with r=0.9992. The detection limit for dopamine was 4 μg/kg and the quantification limit was 9 μg/kg. Recovery studies were carried out at 0.1, 0.5 and 1.0 mg/kg fortification levels and the average recoveries obtained ranged from 90.4% to 98.2% with relative standard deviations between 3.5% and 8.1%. The method was found to be suitable for detection of dopamine in animal product tissues at the maximum residue level.     

基于监控视频的高速公路能见度检测方法及系统

针对我国高速公路能见度天气的全面检测与预警处置的迫切需求,提出了一种基于监控视频的高速公路能见度检测方法.该方法从监控摄像头提取固定距离参照物的图像,根据图像的灰度失真状况计算能见度值,并通过数据融合的手段对多监控摄像头计算结果进行综合;并设计和开发了一款能见度监测与预警处置信息化系统,利用获取的实时能见度数据,从局部现场和宏观路网两个方面支撑能见度预警、应急处置、交通流调控、信息发布等智能交通业务.实验分析结果表明,该能见度计算方法的平均检测误差为13.4%,能够满足我国高速公路能见度检测的精度和计算性能要求;本文方法及系统可以充分复用高速公路沿线广泛架设的视频监控机电系统,可应用于我国高速公路的运营管理及效率提升等工作中.      

Less is More： Data Processing with SVM for Intrusion Detection

To improve the detection rate and lower down the false positive rate in intrusion detection system,dimensionality reduction is widely used in the intrusion detection system.For this purpose,a data processing (DP) with support vector machine (SVM) was built.Different from traditionally identifying the redundant data before purging the audit data by expert knowledge or utilizing different kinds of subsets of the available 41-connection attributes to build a classifier,the proposed strategy first removes the attributes whose correlation with another attribute exceeds a threshold,and then classifies two sequence samples as one class while removing either of the two samples whose similarity exceeds a threshold.The results of performance experiments showed that the strategy of DP and SVM is superior to the other existing data reduction strategies (e.g.,audit reduction,rule extraction,and feature selection),and that the detection model based on DP and SVM outperforms those based on data mining,soft computing,and hierarchical principal component analysis neural networks.     

基于机器视觉的公路交通标志自动化巡检系统

针对既有交通标志巡检的调查手段和分析方法存在的成本高、效率低、精度低的弊端,提出了一种基于机器视觉的公路交通标志自动化巡检系统,实现对公路交通标志的移动式实时检测与分析。首先利用YOLO （You Only Look Once） 算法,将机器视觉技术运用到交通标志的检测中,加快了检测速度。然后给出了交通标志自动化巡检系统的系统构架、系统结构与功能及数据管理方案。最后利用该系统进行实测。实测结果显示,该自动化巡检系统能够准确地检测交通标志、道路标线及路面坑塘,具有较高的测试精度及较好的测试效果。     

用PCR法检测胸液中结核杆菌DNA

利用聚合酶链反应（ＰＣＲ）技术检测了３２例结核性渗出性胸膜炎和２０例非结核性疾病患者胸液中结核杆菌的６５ＫＤａ抗原蛋白编码基因的特异ＤＮＡ片段。结果表明，其检出率为７８．１３％，并具高度特异性。提示ＰＣＲ扩增ＤＮＡ技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。     

A Machine Vision System for Ball Grid Array Package Inspection

An optical inspection method of the Ball Grid Array package (BGA) was proposed by using a machine vision system. The developed machine vision system could get main critical factors for BGA quality evaluation, such as the height of solder ball, diameter, pitch and coplanarity. The experiment has proved that this system is available for BGA failure detection.     

高速公路自动事件检测算法

高速公路事件检测是事件管理中十分重要的1个环节.自动检侧因其成本低、不受天气 限制且检测率高而在事件检侧方法中备受关注. 其中自动检测系统的关键部分是事件检测算 法. 在分析比较各种检测算法的原理和特点及其优缺点的基础上,提出更为有效的事件检测方 法.     

Electrocatalytic oxidation and determination of dopamine at a carbon ionic liquid electrode modified with nafion-L-aspartic acid composite film

The electrocatalytic oxidation of dopamine (DA) was studied by electrochemical approaches at a carbon ionic liquid electrode (CILE) modified with the composite film of nafion and L-aspartic acid (NL-CILE). The CILE was fabricated by replacing non-conductive organic binders with a room-temperature hydrophobic ionic liquid, 1-butyl-3-methyl-imidazolium hexafluorophosphate. The composite film of NL was used as matrix to adsorb DA and catalyze the oxidation of DA in phosphate buffer solution (PBS). The electrochemical response of DA was investigated at the NL-CILE, the traditional carbon paste electrode (TCPE), CILE and the nafion modified CILE (N-CILE) in 0.1M PBS (pH 7.4), respectively. The results showed the superiority of NL-CILE to N-CILE, CILE and TCPE in terms of provision of higher sensitivity, faster electron transfer and better reversibility. Under optimum condition, the oxidation peak current was rectilinear with DA concentration range from 0.1μM to 0.1mM, with a detection limit of 0.03μM (S/N=3) by differential pulse voltammetry. The proposed method was applied to determine DA in samples successfully.     

TGFβ1-(78-109)-HBc融合蛋白的原核表达

目的　应用基因工程技术制备TGFβ1 (78- 10 9) HBc融合蛋白 ,作为TGFβ1疫苗 ,用于抗纤维化研究。方法　合成TGFβ1 (78- 10 9)编码区DNA ,连接于质粒载体 ,再在其下游连接HBc编码区 ,测序证实后 ,进行原核表达 ,表达产物以SDS PAGE、ELISA等方法鉴定。结果　经DNA序列分析证实融合基因序列完全正确 ,SDS PAGE证实原核表达产物相对分子质量为 2 5kD ,ELISA检测证实TGFβ1 (78- 10 9)和HBc的抗原表位均可正确暴露。结论　本研究成功构建了TGFβ1 (78- 10 9) HBc融合基因 ,进行了原核表达 ,并初步证实了表达产物的抗原性 ,为下一步进行TGFβ1疫苗抗纤维化的研究打下了基础     

基于金字塔多尺度融合的交通标志检测算法

为了解决传统交通标志检测算法针对小目标交通标志检测时存在误检与漏检的问题,提出了一个基于金字塔多尺度融合的交通标志检测算法;为了提高算法对交通标志的特征提取能力,引入ResNet残差结构搭建算法的主干网络,并增加网络浅层卷积层数,以提取较小尺度交通标志目标更准确的语义信息;基于特征金字塔结构的思想,在检测结构中引入4个不同预测尺度,增强深层和浅层特征融合;为了进一步提高算法检测精度,引入GIoU损失函数定位交通标志的锚点框,利用k-means算法对交通标志标签信息进行聚类分析并生成更精准的先验框;为了验证算法的泛化性与解决试验所用数据集TT100K的类间不平衡问题,增强与扩充了数据集。试验结果表明：本文算法的精确率、召回率与平均精度均值分别达到了86.7%、89.4%与87.9%,与传统目标检测算法相比有显著提高;多尺度融合检测机制、GIoU损失函数与k-means的引入能够不同程度提高算法的检测性能,使算法检测精确率分别提升4.7%、1.8%与1.2%;提出算法针对不同尺度交通标志检测时均有更优越的性能表现,在TT100K数据集中的(0,32]、(32,96]与(96,400]尺度下...     

基于GNSS/INS的列车定位风险评估方法

全球卫星导航系统（global?navigation?satellite?system，GNSS）应用于列车运行控制系统位置服务时，需对其进行风险评估，以确保其满足安全相关的需求. 为此，首先建立一种基于卫星导航系统与惯导系统（inertial?navigation system，INS）融合的列车定位单元结构，通过分析传感器融合数据对故障进行检测及识别，并计算水平保护距离，结合水平位置误差、水平告警门限、告警时间等指标参数，对列车定位单元的工作状态进行识别；其次在此基础上分析由危险状态生成的风险事件，并计算列车定位单元危险侧失效率及故障概率；最后结合现场试验数据对所提出的风险评估方法进行测试验证. 验证结果表明:若误警率、漏检率均为1 × 10?7/h，水平告警门限为20 m，定位单元在相对开阔环境下的故障率为9.14 × 10?7/h，受限环境下的故障率为1.52 × 10?4/h；若运行线路对风险指标参数需求降低，则误警率、漏检率及水平告警门限也会增大，受限环境下的定位单元故障率也随之降低，在误警率、漏检率均为1 × 10?5/h，水平告警门限为100 m时，计算获得的受限环境下定位单元故障率为0. 因此，在对定位单元进行风险评估时需考虑不同线路对指标参数的需求.      

抵御单粒子多位翻转的系统自恢复技术

为减少多位翻转(multi-bit upset,MBU)对星载计算机的危害,提出了一种抵御单粒子多位翻转的系统自恢复技术.该技术利用硬件EDAC(error detection and correction)检测多位错的能力,结合系统自恢复的容错技术实现MBU的捕获,并选择性地启动系统自恢复,以防止MBU造成的系统安全性问题.通过建立关键数据查询,避免不必要的系统自恢复,采用除法散列法和适度恢复策略提高处理速度.SEU(single event upset)危害性分析以及某卫星在轨SEU观测数据表明,提出的系统自恢复技术可使SEU引起卫星故障的概率下降90%以上.该技术已成功地应用于我国XX02卫星.     

Research of Consistency Maintenance Mechanism in Real-Time Collaborative Multi-View Business Modeling

Real-time collaborative editing(RTCE)can support a group of people collaboratively work from distributed locations at the same time.However,consistency maintenance is one key challenge when different types of conflicts happen.Therefore a common synchronous mechanism is proposed to support consistency maintenance in the process of multi-view business modeling.Based on operation analysis on different views of models in the real-time collaborative editing system,detection of potential conflicts is realized by means of a decision-making tree.Then consistency maintenance provides a comprehensive and applicable conflicts detection and resolution for collaborative business modeling.Finally,a prototype of collaborative multi-view business modeling system is introduced to verify the approach.The point is that the mechanism proposes a comprehensive solution for collaborative multi-view business modeling.