Capillary electrophoresis with inhibited electrochemiluminescent detection for the trace analysis of epinephrine and dopamine

In this paper, a novel electrochemiluminescent (ECL) detection approach was developed for highly sensitive detection of ECL inhibitors based on the ECL inhibition of Ru(bpy)32+/2-(Dibutylamino)ethanol (DBAE) system. A microfluidic ECL detection cell was fabricated to couple with the capillary electrophoresis system, the electrochemical system and the postcolumn injection system. Both Ru(bpy)32+ and DBAE solutions were injected directly to the working electrode surface by a micro-infusion system to obtain a high and stable ECL signal. The performance of this setup was demonstrated by the analysis of two typical ECL inhibitors, dopamine and epinephrine. Under the optimal conditions, the limit of detection (LOD) for dopamine and epinephrine was 50nM and 5nM respectively. The proposed method was also successfully used for the trace analysis of dopamine and epinephrine in human serum samples.     

CE-ECL法同时测定复方磷酸可待因中三种成分的方法学研究

目的 建立毛细管电泳-电化学发光法分离并检测复方磷酸可待因中三种成分,磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱含量的新方法.方法 基于碱性介质中,复方磷酸可待因溶液中三种主要成分对联吡啶钌在铂电极上的电化学发光信号的增敏作用,且增敏强度和药物浓度成线性关系,与毛细管电泳联用,建立了毛细管电泳-电化学发光法分离并检测其含量的新方法.结果 在优化的实验条件下,确定三种药物对照品的线性范围、线性方程和检出限.通过对浓度为1.0×10~(-5)kg/L的磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱对照品进行12次平行测定,其ECL发光强度的RSD值分别为2.89%,3.76%,3.32%.结论 该方法可用于复方磷酸可待因口服溶液中三种主要成分的含量测定,待测样品中的基质不干扰测定,方法的回收率分别为100.4%,101.9%,99.8%(n=5).     

毛细相变流体回路中蒸发器的预热启动特性研究

通过分析毛细管内液柱的动量方程,从力平衡的角度获得毛细相变流体回路系统的启动判别条件,并建立蒸发器的三维导热模型来研究系统的启动特性.研究表明:蒸发器的启动预热时间随启动热流的增大而减小;减小液体补偿腔的高度可以加快蒸发器的启动过程,且启动热流愈小该现象愈显著;在较低的启动热流条件下,减小蒸气槽道的宽度,可以减小启动预热时间,改善蒸发器启动性能,而在较高的热流密度下,其对蒸发器的启动性能影响不大.     

毛细管内径对类氖氩46.9nm激光输出的影响

毛细管放电类氖氩等离子体产生的46.9 nm激光是亮度很高的相干光源,有着重要应用前景。影响激光输出的放电参量和制约因素较多,其中毛细管内径属于后者。实验研究了内径分别为3 mm和4 mm的氧化铝陶瓷毛细管输出激光的情况,结果表明在满足3 mm内径的毛细管能输出较强的激光信号的最优放电条件下,4 mm内径的毛细管没有输出激光信号。分析实验现象并与理论结论对照,发现4 mm内径的毛细管中氩等离子体被箍缩到出光状态,主脉冲电流应提高4/3倍,达到约53 kA。     

Simultaneous determination of berberine, matrine and oxymatrine in traditional Chinese medicines by using nonaqueous capillary electrophoresis

A rapid method for the simultaneous determination of berberine (BBR), matrine (MT) and oxymatrine (OMT) by nonaqueous capillary electrophoresis (NACE) was developed. Optimum separation of the analytes was obtained on a 50cm×50μm i.d. fused-silica capillary using a non-aqueous buffer system of 70mM ammonium acetate, 7.0% acetic acid and 10% acetonitrile at 25kV and 20℃. The relative standard deviations (R.S.D.) of the migration times and peak areas of the three active components were 0.06%-0.20% and 0.12%-3.41% for berberine, 0.11%-0.60% and 0.74%-1.63% for matrine, 0.15% and 0.45% for oxymatrine, respectively. Detection limits of berberine, matrine and oxymtrine were 0.18μg/mL, 4.08μg/mL and 4.16μg/mL, respectively. In the tested concentration range, good linear relationships (0.9992 for berberine, 0.9988 for matrine and 0.9988 for oxymatrine) were observed. The linear calibration ranges were 0.45-360.0μg/mL for berberine, 8.16-408.0μg/mL for matrine and 20.8-416.0μg/mL for oxymatrine. This method has been successfully applied to the phytochemical analysis of alkaloids extracts from two commonly used traditional Chinese herbal drugs: Sophora flavescens Ait. (Kushen) and Cortex phellodendri chinensis (Huangbai) and their medicinal preparations.     

绝热毛细管流动特性的模拟分析

在绝热毛细管积分近似模型解析解和雍塞状态下压力和流量的关系基础上,提出了结合绝热毛细管积分模型对其流动特性及其相关参数进行计算的研究方法。通过与相关试验和文献结果的比较分析,证实了所建立的模型具有可靠的计算精度和适用性。     

绝热毛细管流动特性的模拟分析

在绝热毛细管积分近似模型解析解和雍塞状态下压力和流量的关系基础上,提出了结合绝热毛细管积分模型对其流动特性及其相关参数进行计算的研究方法。通过与相关试验和文献结果的比较分析,证实了所建立的模型具有可靠的计算精度和适用性。     

硫酸盐盐渍土中毛细水上升规律的初探

在对盐渍土地区毛细水上升机理的研究过程中,首先论述了硫酸盐盐渍土中影响水分迁移的不同因素,其次通过室内试验,得出不同配比下硫酸盐盐渍土毛细水上升高度的基本规律,最后通过实验对硫酸盐盐渍土地区公路病害治理方面提出一些措施和建议。     

Analysis of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection

A new method for the determination of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection (MCE-CCD) was proposed. The effects of va...     

毛细管放电类氖氩46.9nm软X光激光谱线的分辨

毛细管放电46.9 nm软X光激光以高温高密度氩等离子体作为激光增益介质,在46.9 nm激光谱线附近会出现大量的背景光谱,这对于进一步研究激光及其应用极为不利.本文实验研究了不同气压下毛细管放电输出激光的情况,发现低气压下适于产生激光的氩气气压为38 Pa,此时激光信号最大.随着气压的增加,激光信号减小.当气压达到6...     

多敏灵片中咖啡因和盐酸多塞平含量的测定

目的建立高效液相色谱法,测定多敏灵片中咖啡因、盐酸多塞平的含量。方法采用高效液相色谱法,以Kromasil C18(250 mm×4.6 mm,5μm)为分析柱,流动相为甲醇-水-三乙胺(75∶25∶1),流速1 mL/min,检测波长295 nm,柱温为室温。结果咖啡因、盐酸多塞平与其他组分分离良好,且不受其他成分的干扰;咖啡因回归方程为:Y=196 604X+218 976(r=0.999 8,n=5),咖啡因在0.08~0.24μg的范围内线性关系良好;盐酸多塞平回归方程为:Y=60 158X+58 230(r=0.999 9,n=5),盐酸多塞平在0.04~0.12μg的范围内线性关系良好。方法的平均回收率分别为99.06%、96.67%,精密度(RSD)值分别为1.04%、1.13%。结论本方法操作简便、快速、准确、灵敏,适用于多敏灵片的含量测定。     

Normal and reverse flow injection-spectrophotometric determination of thiamine hydrochloride in pharmaceutical preparations using diazotized metoclopramide

Simple and sensitive normal and reverse flow injection methods for spectrophotometric determination of thiamine hydrochloride (THC) at the microgram level were proposed and optimized. Both methods are ...     

应用离心造粒法制备盐酸二甲双胍微丸的研究

目的研究离心造粒粉末层积法制备盐酸二甲双胍微丸。方法采用多功能微丸包衣造粒机制备微晶纤维素空白丸芯和盐酸二甲双胍微丸,并且通过产率、粒径和粒径分布、堆密度、表面形态和圆整度、脆碎度和释放度对盐酸二甲双胍微丸进行质量评价。结果以盐酸二甲双胍∶乳糖∶微晶纤维素=100∶5∶3的比例,主机转速150 r/min,喷浆泵转速20 r/min,供粉速度25 r/min,滚圆6 min,进行制备微丸,获得了很好的效果。结论所制备的盐酸二甲双胍微丸圆整度好,粒径均匀,硬度合格,流动性好,释放完全,无骨架结构。     

轻轨车辆电气耦合轮对导向控制技术

针对独立车轮自导向性能差的问题,应用改进电轴技术在轻轨车辆电机单独驱动独立车轮中,形成一种电气耦合轮对型式,以无形的电轴替代传统的机械轴,改善导向性能.从电路分析理论出发,分析了新型耦合轮对的耦合性能和影响因素,确定电气耦合轮对是一种对左右车轮转角差自动施加反馈控制的技术,且轮对耦合的强弱可通过调节电容与电阻实现,调节电容方案因能耗低而优于调节电阻方案.建立电气耦合轮对和弹性阻尼耦合轮对轻轨车辆横向动力学模型,分析了轮对的转向行为.仿真结果表明:电气耦合轮对耦合力矩最大达到近1kN·m,耦合能力强;直线上受激扰后,2种车辆的横移量与摇头角自行趋于0,通过曲线时,横移量稳定值小于8 mm,摇头角小于0.2°,因此,2种耦舍轮对车辆具有直线对中能力和曲线通过性能;通过小曲线时,电机因电气耦合而造成的最大耗能功率低于140 W,系统能耗低.     

Liquid chromatography coupled with time-of-flight and ion trap mass spectrometry for qualitative analysis of herbal medicines

With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing g...     

Sensing of chlorpheniramine in pharmaceutical applications by sequential injector coupled with potentiometer

This paper reports on development of a system consisting of a portable sequential injector coupled with potentiometric unit for sensing of chlorpheniramine （CPA）, based on the reaction of CPA with pota...     

弹性阻尼耦合轮对铁路客车系统横向稳定性

首次将弹性阻尼耦合轮对应用到客车系统中 ,并采用特征值法对该系统横向稳定性进行了分析。指出将弹性阻尼耦合轮对模型的扭转刚度和扭转阻尼分别取不同的值 ,可与阻尼耦合轮对、扭转弹性轮对以及独立轮对模型统一。研究结果表明 ,采用弹性阻尼耦合轮对模型 ,选取适当的扭转阻尼和扭转刚度 ,可以提高车辆蛇行临界速度。     

跨座式单轨交通系统耦合振动特性

将列车的每节车厢简化为15自由度的动力系统, 由拉格朗日方程导出其振动微分方程, 将轨道梁简化为欧拉梁, 基于能量法和车辆与轨道梁位移协调条件, 建立了车辆和轨道梁耦合运动控制方程, 研究了跨座式单轨交通轨道梁的动力特性。计算了列车以不同车速通过时轨道梁的动力响应, 并比较了计算结果与实测结果。比较结果表明: 理论计算结果与实测结果基本吻合, 车速对轨道梁挠度的影响较小, 但对加速度影响较大, 加速度随车速增大而增大, 在40~50 km·h^-1处出现最大值, 之后随车速增大反而减小; 轮胎模型与轨道表面不平度功率谱密度函数对轨道梁横向响应计算结果影响较大, 使计算误差增大。