Simultaneous determination of captopril and hydrochlorothiazide by time-resolved chemiluminescence with artificial neura

The combined use of chemometrics and chemiluminescence（CL）measurements,with the aid of the stopped-flow mixing technique,developed a simple time-resolved CL method for the simultaneous determination of captopril（CPL）and hydrochlorothiazide（HCT）.The stoppe     

一种新的循环谱统计量频谱感知算法

为了提高认知无线电中感知用户对授权用户（主用户）的检测概率,提出一种新的基于循环谱统计量的主用户信号检测算法．该算法利用循环谱离散频域平滑方法,对信号的循环谱在循环频率处建立合理的检测统计量,然后求解得到对应不同虚警概率的判决门限,以使感知用户能检测信噪比更低的授权用户信号．该算法具有检测结构简单,易实现的特点．仿真结果表明,在高斯白噪声信道中,虚警概率为0．1,频域平滑累积次数为255,在信噪比为-16dB的条件下,与Dandawate-Giannakis（DG）方法相比,本文算法的检测概率提高23％．     

产孢海洋真菌等离子体诱变活性突变株筛选改进

以具有抗肿瘤抗菌活性的产孢子海洋真菌Aspergillus unguis DLEP2008001为出发菌株,对其采用介质阻挡放电等离子体技术进行了诱变处理.在采用肿瘤细胞替代模型——DNA损伤修复基因缺陷型的大肠杆菌E coli AB3027进行突变株活性筛选的过程中,比较了三种筛选方案,最终确定了一种使用96孔板液体摇床发酵的筛选方法,该方法与通常的液体深层发酵有较好的对应性,且方便易行.通过该方法,灵敏快捷的筛选出了基于DNA损伤机制的高活性突变株,同时也显示介质阻挡放电等离子体技术对提高该菌株生物活性具有显著效果.     

Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatoceUular carcinoma marker glypican-3

Glypican-3 （GPC3） is reported as a great promising tumor marker for hepatocellular carcinoma （HCC） diagnosis. Highly sensitive and accurate analysis of serum GPC3 （sGPC3）, in combination with or instead of traditional HCC marker alpha-fetoprotein （AFP）, is essential for early diagnosis of I-ICC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles （MnPs） and magnetic microparticles （MmPs） with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay （CLEIA）. After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance.     

Chemiluminescence determination of melamine with Luminol-K3Fe（CN）6 system

A sensitive chemiluminescence（CL） method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe（CN）6 system in alkaline medium. The determination con     

基于简化差分相干积累的北斗B1频点信号精捕获算法

北斗卫星信号的捕获速度、灵敏度及精度将直接影响接收机的性能指标.本文提出了一种提高北斗导航卫星B1频点信号检测信噪比的精捕获算法,该算法基于简化差分相干积累（SDCI）的短时匹配滤波器（STMF）和快速傅里叶变换（FFT）运算（STMF-FFT）.为了保证在降低FFT运算量和提高信号检测概率的条件下,获得更精确的多普勒频率估计,采用逼近效果较好的切比雪夫线性最小二乘曲线拟合法得到多普勒频移的精确化估计值.理论分析和仿真验证表明：本文提出的SDCI算法比非相干积累算法获得的检测信噪比高约3.2 dB.     

Flow injection chemiluminescence determination of loxoprofen and naproxen with the acidic permanganate-sulfite system

A novel flow injection chemiluminescence（CL）method for the determination of loxoprofen and naproxen was proposed based on the CL system of KMnO4 and Na2SO3 in acid media.The CL intensity of KMnO4-Na2SO3 was greatly enhanced in the presence of loxoprofen a     

我国运输服务业国际竞争力现状分析

通过对我国运输服务业的贸易现状,市场占有率、显性比较优势指数、贸易竞争力,以及法律环境、硬件设施等方面来综合测度和分析我国运输服务业的国际竞争力,得出其法律环境、硬件设施不断完善,但总体国际竞争力低下的重要结论.     

Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin （HSA） was investigated using indirect chiral high performance liquid chromatography （HPLC） and ultrafiltration techniques. S-（-）-1-（1-naphthyl）- ethylamine （S-NEA） was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of S-enantiomer was stronger than R-enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.     

朔黄重载铁路路桥过渡段加固路基的检测与分析

在既有重载铁路中,路基、桥梁与涵洞等构筑物连接的路桥过渡段是线路的薄弱环节,对其进行检测,评估路基承载力是保证线路平顺度和列车安全、平稳运行的关键.采用地基系数K30试验、轻型动力触探试验和室内试验对加固后的朔黄铁路第170号桥路桥过渡段质量进行了检测.试验结果表明：①地基系数K30能够直观地表征路基刚度及承载能力,加固后朔黄铁路170号桥路桥过渡段不同位置处的地基系数K30值提高15%～40%左右;②地基系数K30和轻型动力触探试验结果N10有显著的线性关系,压实系数与地基系数K30不存在相关性;③既有重载铁路路桥过渡段路基质量检测应进行大量的轻型动力触探试验,再辅以地基系数K30试验进行校核,对提高检测的工作效率、降低检测成本、减少检测工作对既有线运营的干扰有重要的意义.     

基于STBC的混沌MIMO雷达的信号处理研究

针对MIMO（multiple input multipleoutput）雷达正交信号设计的困难,提出了一种超宽带调制的混沌二相码,并结合STBC（space-time block coding）技术,能够达到发射信号的完全正交和最大空间分集．方法简单易行,具有较好的保密特性．仿真实验给出2×2系统下,信噪比为20dB时的回波信号波形,发现该方法较好地实现对复杂目标的识别．同时推导了该模型在奈曼皮尔逊准则下的目标检测概率,相对于窄带系统,检测概率显著提高．     

Microfluidics-based assay on the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors

Abstract A new microfluidic system with four different microchambers （a circle and three equilateral concave polygons） was designed and fabricated using poly（dimethylsiloxane） （PDMS） and the soft lithography method. Using this microfluidic device at six flow rates （5, 10, 20, 30, 40, and 50 μL/h）, the effects of microenvironmental geometry and aqueous flow on bacterial adhesion behaviors were investigated. Escherichia coli HB101 pGLO, which could produce a green fluorescent protein induced by L-arabinose, was utilized as the model bacteria. The results demonstrated that bacterial adhesion was significantly related to culture time, microenvironment geometry, and aqueous flow rates. Adhered bacterial density increased with the culture time. Initially, the adhesion occurred at the microchamber sides, and then the entire chamber was gradually covered with increased culture time. Adhesion densities in the side zones were larger than those in the center zones because of the lower shearing force in the side zone. Also, the adhesion densities in the complex chambers were larger than those in the simple chambers. At low flow rates, the orientation of adhered bacteria was random and disorderly. At high flow rates, bacterial orientation became close to the streamline and oriented toward the flow direction; All these results implied that bacterial adhesion tended to occur in complicated aqueous flow areas.The present study provided an on-chip flow system for physiological behavior of biological cells, as well as provided a strategic cue for the prevention of bacterial infection and biofilm formation.     

基于形态学分形维数的输电线路故障选相方法

输电线路故障信号是一种典型的非线性信号,分形几何理论为描述非线性故障信号的特性提供了一个有力的分析工具。针对传统分形维数的局限性,本文提出了一种基于局域均值分解（local mean decomposition,LMD）-形态学的分形维数-Elman神经网络的输电线路故障选相新方法。该方法通过对故障电流进行相模转换后,对单一线模分量进行LMD分解得到若干乘积函数（product function,PF）分量,然后选取前4个PF分量进行数学形态学的分形维数估计,最后形成特征向量作为Elman神经网络的输入参数。仿真试验表明：提出的故障分类识别方法能快速、准确地识别各类故障,并且不易受故障初始角、故障位置和过渡电阻的影响,与传统的BP神经网络相比,Elman神经网络具有更好的效果,为准确判断输电线路故障选相提供了一种快速有效的新方法。     

一种在OFDM系统中同步训练序列结构优化方法

同步对于正交频分复用（OFDM）性能至关重要,由于经典Schmidl＆Cox同步算法在符号定时同步估计中,存在同步估计不准确性问题,故对训练序列的结构进行优化,以提高系统性能.     

基于小波变换的图像水印研究

研究了一种新的基于小波变换的数字水印技术。该项技术中,把强健水印嵌入至一幅图像中频率最低的部分,用于检测图像是否发生改变的脆弱水印则在较为粗糙的尺度下,通过对小波系数设置阈值及量化的方法嵌入到图像中。实验表明,强健水印具有抵抗非恶意图像变换（如有损压缩等）,而脆弱水印能检测到恶意的图像攻击（如修改或伪造图像中的某一部分）。     

Preparation and evaluation of monolithic molecularly imprinted stationary phase for S-naproxen

An S-naproxen（S-NAP）molecularly imprinted monolithic stationary phase（MIMSP）with specific recognition for S-NAP and naproxen（NAP）was prepared by in situ technique,utilizing 4-vinylpridine（4-VP）as a function monomer,ethylene glycol dimethacrylate（EDMA）as a     

单相SAPF系统谐波电压检测与控制的仿真

为了改善用户侧电压质量与谐波问题,通过分析单相串联型有源电力滤波器（SAPF）的结构、原理及特点,提出了单相SAPF系统谐波检测的方法;同时借鉴单相SVPWM技术原理,将开关模式优化的SVPWM技术应用于单相SAPF系统中,提高了系统波形控制精度;利用Matlab软件,建立了基于上述谐波检测和系统波形控制的单相SAPF系统的仿真模型,并利用该模型对动态电压跌落与闪变等电压故障状态进行了动态补偿的仿真.仿真结果显示：本文提出的SAPF滤波系统不但具有良好的滤波性能,而且可以保障负载不受系统电压故障的影响,具有良好的工程应用前景.     

基于虚拟装配体绑定的产品层次化装配序列生成

装配是产品制造中的重要环节之一.计算机辅助装配顺序的生成为实施快速、灵活、优化的装配,提高装配质量和效率提供有力的支持.任务层次与分解方法的设计是层次任务网规划方法应用的关键技术.文中提出了一种基于虚拟装配体绑定的底层装配操作实施机制,在此基础上建立了产品装配序列生成的任务分解策略.装配实例表明,该方法在产品装配序列生成中具有建模层次清晰、任务分解策略易于制定、装配序列生成比较灵活的特点.     

自密实再生混凝土的基本力学性能试验研究

为了研究自密实再生混凝土的基本力学性能,通过立方体抗压强度试验、轴心抗压强度试验、劈裂抗拉强度试验和弹性模量试验,观察了自密实再生混凝土的受压和受拉破坏过程和破坏形态,分析了再生粗骨料替代率对自密实再生混凝土基本力学性能的影响,探讨了普通混凝土和再生混凝土的各力学性能指标之间的换算关系对自密实再生混凝土的适用性. 结果表明:自密实再生混凝土受压和受拉的破坏形态与普通混凝土相似;当再生粗骨料替代率由0增加至100%时,自密实再生混凝土的立方体抗压强度、轴心抗压强度、劈裂抗拉强度和弹性模量分别降低了15.5%、12.7%、25.6%和11.5%,而自密实再生混凝土的峰值应变增大了19.8%;再生粗骨料替代率对自密实再生混凝土的泊松比无显著影响;普通混凝土和再生混凝土的各力学性能指标之间的换算关系不适用于自密实再生混凝土,提出的自密实再生混凝土的立方体抗压强度与其他力学性能指标之间的换算关系具有较高精度.      

A New Range Sidelobe Suppression Technique for Randomly Intermittent Spectra HF Radar Signal

The randomly intermittent spectra (RIS) signal is employed to combat spectrum congestion in radar and other radio services to evade the external interferences in high-frequency (HF) and ultrahigh-frequency (UHF)bands. However, the spectra discontinuity of the signal gets rise to high range sidelobes when matching the reflected echo, which is much more difficult for targets detection. So it is indispensable to investigate the technique for sidelobes suppression of the range profile when RIS signal is utilized, This paper introduced a new processing technique based on time domain filtering to lower the range sidelobes. A robust and effetive algorithm is adopted to solve the coefficients of the filter, and the restriction on the desired response of the filter is derived. The simulation results show that the peak range sidelobe can be reduced to -27dB from -9.5dB while the frequency band span (FBS) is 200kHz.