EFFECTS OF QUERCETIN ON MEMBRANE FLUIDITY OF INJURED VASCULAR ENDOTHELIAL CELLS WITH HYPOXIA AND THE LACK OF GLUCOSE

Vascularendothelialcellsinhypoxiaandthelackofglucosecanproducealotofactiveoxygen ,whichresultsinthedamageofendothelialcells .Furthermore,dysfunctionofvascularendothelialcellsistheinitiativestepofatherosclerosisandmayleadtosomeofcardiovasculardiseases[1 ,2 ] .So ,theprotectionofendothelialcellsfromtheinjuryplaysanimportantroleinthepreventionandtreatmentofatherosclerosis,hypertension ,hyperc holesterolaemiaandsoon .Therefore,manyre searchershavebeenlookingforamedicinethatcanhelptomaintainthenorm…     

STUDY ON LIQUID-LIQUID EXTRACTION OF RESVERATROL FROM GIANT KNOTWEED AND ITS PROTECTIVE EFFECT ON MYOCARDIUM INJURY

Ｒｅｓｖｅｒａｔｒｏｌｈａｓｂｅｅｎｆｏｕｎｄｉｎｍａｎｙｐｌａｎｔｓａｎｄｈａｓａｌｏｔｏｆｖａｌｕａｂｌｅｅｆｆｅｃｔｓｕｃｈａｓａｎｔｉ ｔｕｍｏｒ,ａｎｔｉ ｉｎｆｌａｍ ｍａｔｉｏｎ ,ｔｒｅａｔｍｅｎｔｏｆｃａｒｄｉｏ ｖａｓｃｕｌａｒｄｉｓｅａｓｅｓ[1] .Ｔｈｅｍｅｃｈａｎｉｓｍｉｓｒｅｌａｔｅｄｔｏｔｈｅｑｕｅｎｃｈｉｎｇｏｆｌｉｐｉｄｐｅｒｏｘｉｄａ ｔｉｏｎ[2 ,3] .Ｍａｎｙｐａｐｅｒｓｒｅｐｏｒｔｅｄｔｈａｔｒｅｓｖｅｒａｔｒｏｌｗａｓｅｘ ｔｒａｃｔｅｄｆｒｏｍｇｒａｐｅ .Ｈｏｗｅｖ…     

THE PROTECTIVE EFFECTS OF THE TOTAL SAPONIN OF DIPSACUSASPEROIDES ON THE APOPTOSIS OF HIPPOCAMPAL NEURONSINDUCED BY β-AMYLOID PROTEIN

Alzheimer sdisease(AD)isaneurodegenerativedisease ,whosehallmarksof pathologyaresenileplaques,βamyloidprotein (Aβ)depositionwith inbrain ,neurofibrillarytanglesformationandalotofneuronalloss .Thefactorsthatleadtoneuronallossareofcomplexityandmultiplicity ,includingthetoxicityofAβ,intracellularfreecalciumcon centrationhomeostasisimbalance ,damageofoxidefreeradicals ,andinitiationofmechanismofapop tosis,etal.[1- 3] .Thedetailedmechanismhasnotreachedadefiniteconclusion .Thepreventionandtreat…     

EFFECTS OF HYPOPHYSECTOMY AND GROWTH HORMONE REPLACEMENT ON HYPOTHALAMIC SOMATOSTATIN IN THE OLD RAT

ＥＦＦＥＣＴＳＯＦＨＹＰＯＰＨＹＳＥＣＴＯＭＹＡＮＤＧＲＯＷＴＨＨＯＲＭＯＮＥＲＥＰＬＡＣＥＭＥＮＴＯＮＨＹＰＯＴＨＡＬＡＭＩＣＳＯＭＡＴＯＳＴＡＴＩＮＩＮＴＨＥＯＬＤＲＡＴＺｈａｎｇＺｈａｎｇｊｉｎ；ＲｅｎＨｕｉｍｉｎ；ＨｕＨａｉｔａｏ；ＬｉｎｇＦ...     

DESENSITIZATION OF ACETYLCHOLINE ON THE INHIBITION EFFECTS OF BLOOD PRESSURE IN ANESTHETIZED CANINE

Desensitizationisaphenomenonthatthefirstreactiontowardsadrugdecreasedduringoraftercontinuouslyexposure[1] .Now ,theconceptioniswidelyusedtorepresentthephenomenonthatthereactionoftargettissuesorcellstowardshormone ,drugorneurotransmitterisreduced gradually[2 ] .Themechanismofdesensitizationisstillunclear .Itiswellknownthattheeffectsofheart,autonomicneuralsystem ,muscles ,arefadewhenexposuretoacetylcholine (ACh)foraperiodoftime[3] .Atpre sent,manystudiesfocusonisolatedtissuesorcells[1,5,6 ,7,8]…     

EFFECTS OF THE HYPERTHERMIA AND HARRINGTONINE ON TELOMERASE ACTIVITY OF HL-60 CELLS

ＴｅｌｏｍｅｒａｓｅｉｓａｎＲＮＡｄｅｐｅｎｄｅｎｔｐｏｌｙｍｅｒａｓｅｔｈａｔｓｙｎｔｈｅｓｉｚｅｓｔｒｌｏｍｅｒｅＴＴＡＧＧＧｒｅｐｅａｔｓｓｅｑｕｅｎｃｅｓａｔｔｈｅｅｎｄｓｏｆｃｈｒｏｍｏｓｏｍｅｓｒｅｑｕｉｒｅｄｆｏｒｔｈｅｉｎｔｅｇｒｉｔｙａｎｄｓｔａｂｉｌｉｔｙｏｆｌｉｎｅａｒｃｈｒｏｍｏｓｏｍｅｓ[1] .Ｎｏｒｍａｌｓｏｍａｔｉｃｃｅｌｌｓ ,ｇｅｎｅｒａｌｌｙｌａｃｋｉｎｇｔｅｌｏｍｅｒａｓｅａｃｔｉｖｉｔｙ ,ｄｉｓｐｌａｙｐｒｏｇｒｅｓｓｉｖｅａｔｔｒｉｔｉｏｎｏｆｔｅｌｏｍｅｒ…     

THE DETECTION AND CLINICAL SIGNIFICANCE OF hEGF AND β＿2－MG IN BALF OF PATIENTS WITH LUNG CANCER

ＴＨＥＤＥＴＥＣＴＩＯＮＡＮＤＣＬＩＮＩＣＡＬＳＩＧＮＩＦＩＣＡＮＣＥＯＦｈＥＧＦＡＮＤβ＿２－ＭＧＩＮＢＡＬＦＯＦＰＡＴＩＥＮＴＳＷＩＴＨＬＵＮＧＣＡＮＣＥＲ（陈国安）（刘天菊）（何积银）（李雅莉）（杨德昌）（任晓肖）（郑纯礼）ＣｈｅｎＧｕｏａｎ；...     

EFFECTS OF DIPSACUS ASPER AND VITAMIN E ON THE SS NEURONS IN THE HIPPOCAMPAL FORMATION OF RAT MODELS OF ALZHEIMER‘S DISEASE

Ａｌｚｈｅｉｍｅｒ’ｓｄｉｓｅａｓｅ (ＡＤ)ｉｓｔｈｅｍｏｓｔｃｏｍｍｏｎｄｅｍｅｎｔｉａｉｎｔｈｅｓｅｎｉｏｒ ,ａｎｄｔｈｅｒｅｉｓｎｏｃｕｒｅｏｒｅｆｆｅｃ ｔｉｖｅｔｒｅａｔｍｅｎｔ .ＡｌｔｈｏｕｇｈｔｈｅｅｔｉｏｌｏｇｙｏｆＡｌｚｈｅｉ ｍｅｒ’ｓｄｉｓｅａｓｅｉｓｎｏｔｆｕｌｌｙｕｎｄｅｒｓｔｏｏｄ ,ａｃｃｕｍｕｌａｔｉｎｇｅｖｉｄｅｎｃｅｄｅｍｏｎｓｔｒａｔｅｓｔｈａｔｂｏｔｈｔｈｅｃｏｎｃｅｎｔｒａｔｉｏｎｏｆｔｈｅｓｏｍａｔｏｓｔａｔｉｎ (ＳＳ)ａｎｄｔｈｅＳＳｎｅｕｒｏｎｓｉｎｔｈ…     

EFFECTS OF CHRONIC STRESS ON THE ACTIVITIES OF SOD, GSH-Px AND MDA LEVEL IN FEMALE RATS‘ BRAIN

“ＣｉｔｙＳｔｒｅｓｓＳｙｎｄｒｏｍｅ” ,ｓｕｃｈａｓａｎｘｉｅｔｙ ,ｍｅｍｏｒｙｒｅｃｅｓｓｉｏｎａｎｄｎｅｕｒａｓｔｈｅｎｉａ ,ｉｓｂｅｃｏｍｉｎｇａ ｇｌｏｂａｌｐｈｅｎｏｍｅｎｏｎ .Ｏ’Ｂｒｉｅｎｒｅｐｏｒｔｅｄｔｈａｔ ｐｒｏｌｏｎｇｅｄｓｔｒｅｓｓｍｉｇｈｔｃａｕｓｅ ｐｅｒｍａｎｅｎｔｂｒａｉｎｄａｍａｇｅ[1] .Ｑｕｅｒｖａｉｎ’ｓｒｅｓｅａｒｃｈｅｓｉｎｄｉｃａｔｅｄｔｈａｔｃｈｒｏｎｉｃｓｔｒｅｓｓｃｏｕｌｄｉｍｐｒｏｖｅｍｅｍｏｒｙｄｅｆｉｃｉｅｎｃｙｍｅｄｉａｔｅｄｂｙｈｉｐｐ…     

EFFECTS OF THROMBOLYSIS WITH ULTRASOUND ON THE STRUCTURE OF ERYTHROCYTE AND ITS SAFETY THRESHOLD

The study was to investigate thrombolysis in vitro with ultrasound, and to discuss effects of thrombolysis with ultrasound on the structure of erythrocyte and its safety threshold under different ultrasound intensity and exposure time. The structure of erythrocyte in thrombus was evaluated under light microscope. The relationship between the structure of erythrocyte in thrombus and ultrasound intensity and exposure time was obtained. The results showed that ultrasound eliminated the thrombus. According to the change of the structure of erythrocyte in thrombus and ultrasound intensity and exposure time, the effects of thrombolysis with ultrasound could be divided into three kinds of areas： the A, B, C area. The area A was the safe area, the area B was the relatively safe area, and the area C was the irreversible damage area. The study suggested that ultrasound intensity and exposure time had significant impact on the structure of erythrocyte. Stronger ultrasound intensity or longer exposure time could cause erythrocytes irreversible damage. It could accelerate thrombolysis and shorten the exposure time that the ultrasound intensity was little bit increased. The study of effects of thrombolysis with ultrasound on the structure of erythrocyte and its safety threshold were important for practical applications.     

扩张型心肌病患者心肌线粒体DNA缺失突变及与心衰发病的关系

为探讨扩张型心肌病（DCM）患者心肌线粒体DNA（mtDNA）缺失突变对心衰发病的意义，10例DCM患者心内膜活检心肌，用定量PCR方法，以常发缺失型突变mtDNA4977bp（mtDNA4977）和7436bp（mtDNA7436）缺失率为损伤指标，观察心衰不同发展阶段mtDNA缺失程度与患者心肌线粒体呼吸酶活性改变及临床心功能受损之间的关系。结果表明：DCM患者活检心肌中mtDNA4977缺失频率为100％，缺失率在0．128％～1．028％之间；mtDNA7436的缺失频率为70％，缺失率为0．103％～0．843％；10例健康对照中，仅2例45岁以上者有低水平的mtDNA4977及mtDNA7436缺失。心功能愈差，心脏扩大愈显著者，mtDNA缺失率愈高，心肌线粒体呼吸酶活性降低程度也愈明显；mtDNA缺失程度与心肌线粒体呼吸酶活性改变及整体心功能受损之间有着良好的一致性关系。提示mtDNA缺失可能参与了DCM心衰的形成与发展过程。     

阿霉素对大鼠心肌细胞膜磷脂损伤及CoQ_(10)的保护作用

用电镜方法观察阿霉素（ADR）对心肌细胞膜磷脂损伤及CoQ10的保护作用。结果显示：ADR大鼠心脏心肌细胞依ADR累积量均呈现线粒体明显增生，线粒体膜磷脂不同程度脱失；血浆及心肌丙二醛（MDA），血浆乳酸脱氢酶（LDH）和肌酸磷酸激酶（CPK）升高，且与心功能及心脏大小相关。培养心肌细胞结构、培养液中MDA、LDH及CPK含量与ADR心脏改变一致。CoQ10干预后，心肌细胞线粒体增生程度减轻，线粒体膜磷脂损伤性改变得到修复。提示：ADR通过损伤心肌细胞及线粒体膜磷脂对心脏产生毒害作用。CoQ10对这种膜损伤有保护和修复作用。     

扩张型心肌病的自身免疫发病机理研究

本文应用淋巴细胞单克隆抗体,发现扩张型心肌病(DCM)外周血淋巴细胞亚群明显异常;同时用短寿命细胞法发现DCM 的抑制细胞功能明显减低,并与心功能不全之间存在明显的正相关。还发现DCM 患者循环中抗心肌抗体对培养的心肌细胞具有明显的细胞毒效应。DCM 可能是一个与病毒性心肌炎及遗传免疫缺陷有关的自身免疫病.     

美多心安对扩张型心肌病患者血淋巴细胞线粒体膜的影响

为探讨美多心安对心力衰竭患者心肌能量代谢的影响，以淋巴细胞为研究模型，用电镜细胞化学方法，对35例扩张型心肌病心衰患者在常规抗心衰治疗基础上随机分为两组，加服美多心安或安慰剂。经平均2．5月观察，随着心功能改善，加用美多心安者外周血淋巴细胞线粒体增生程度减轻，线粒体膜磷脂超微结构定位损伤性改变得到调整、修复；而在常规抗心衰治疗基础上加用安慰剂者，则改变不明显。提示美多心安可通过调整、修复心衰患者线粒体膜磷脂损伤，改善心肌能量代谢状态。     

容量超负荷心脏不同阶段线粒体膜磷脂结构改变及卡托普利干预的影响

对容量超负荷大鼠模型，用电镜细胞化学方法观察了卡托普利不同时期干预对心肌线粒体膜磷脂结构损伤性改变的作用。结果发现在容量超负荷不同阶段，心肌线粒体皆表现有增生及线粒体嵴膜磷脂结构有程度不等的脱失等损伤性改变。于超负荷后随即卡托普利干预者，心肌线粒体膜磷脂结构改变与对照组比较变化不大（Ｐ＞０．０５），心室作功能力却进一步降低；于造瘘后１月干预者，反映心脏代谢状态的心肌线粒体膜磷脂结构损伤性改变有所减轻，同时部分心室作功参数也有所改善。提示在容量超负荷心脏过早应用转换酶抑制剂不利于心脏重建，而于造瘘后一月干预，可改善心肌代谢和心室功能。     

~(99m)TC-MIBI心肌显像对扩张型心肌病的诊断价值

为探讨心肌显像对扩张型心肌病（DCM）的诊断价值，对34例DCM患者和14例以心衰为突出表现的其它心脏增大患者进行99mTc－MIBI心肌灌注断层显像。34例DCM患者表现左室重度扩大者32例（占94％），其中72％（23／32）同时伴有不同程度的右室扩大，心肌出现多节段花斑样改变者占85％；6例缺血性，C肌病（ICM）患者主要表现为轻度和中度的左室扩大；以二尖瓣狭窄为主的风湿性心脏病（RHD）患者主要表现为右室扩大。提示该显像技术可用于DCM的诊断及鉴别诊断。     

左旋多巴治疗扩张型心肌病的远期随访观察

本文对9例扩张型心肌病患者进行了长期口服左旋多巴(L-dopa)治疗,平均随访19.8±7.7个月。结果表明:其中6例病人的心功能得到了显著改善,表现为运动耐力增加,生活能力提高.故认为在扩张型心肌病治疗中,L-dopa 不失为可供选择的药物之一。     

SUPEROXIDE DISMUTASE ACTIVITY AND MEMBRANE LIPIDSCOMPOSITION OF ERYTHROCYTES IN PATIENTS WITH DILATEDCARDIOMYOPATHY

Dilatedcardiomyopathyisakindofunknowncausesheartmuscledisease.Inrecentyears,theroleoflipid-peroxidationcausedbyoxygenfreeradicalsinjuryinthepathogenesisofmyocardialinjuryanddilatedcardiomyopathyhasbeenpaidmuchattention.Ithasbeendemonstratedthattheselenium(Se)content,glutathioneperoxidase(GSH-Px)activitiesdecreasedandtheserumcon-tentofmalondiadehyde(MDA)increasedsignifi-cant1yinthepatientswithdilatedcardiomyopathy.Itcanbesupposedthattheabilityofanti-peroxida-tionhaddecreasedinthosepatients"…     

阿斯匹林对卒中患者血管内皮细胞释放tPA功能的影响

比较了日服40mg（甲组）和300mg（乙组）阿斯匹林对缺血性脑卒中患者静脉血流加压阻断前后血浆组织型纤溶酶原激活物含量（tPA：Ag）、活性（fPA：A）和其抑制物活性（PMI：A）的影响。结果表明：静脉血流加压阻断10min后tPA：Ag升高程度用药后较用药前为低（P＜0．05），但两组间无差异。乙组tPA：A升高程度用药后较用药前为低（P＜0．05），甲组用药前后无差异。提示阿斯匹林能保护内皮细胞，增强其对血流阻断损伤的耐受性。40mg／d阿斯匹林较小影响内皮细胞纤溶活性的储备，可能更有利于年中的预防。     

用无创伤性方法判定扩张型心肌病肺嵌压及平均肺动脉压

本文用超声心动图和心脏X线片有关参数估测了21例扩张型及缺血性心肌病患者肺嵌压(PCWP)及平均肺动脉压(PAP)并与同期漂浮导管测定结果进行了对照。结果显示:用左房内径、心脏面积指数及用左房内径、心脏面积指数和右肺下动脉宽度分别计得的回归方程,可较准确的反映PCWP及PAP。