Distribution of phytoplankton in the southern Black Sea in summer 1996, spring and autumn 1998

The species composition, abundance, and biomass of micro- (>15 μm) and nano- (<15 μm) phytoplankton were studied along the southern Black Sea during June–July 1996 and March–April and September 1998. A total of 150 species were identified, 50% of them being dinoflagellates. The average total phytoplankton abundance changed from 77×10³ cells l⁻¹ in spring to 110×10³ cells l⁻¹ in autumn and biomass from 250 μg l⁻¹ in summer to 1370 μg l⁻¹ in spring. Based on the extensive sampling grid from June–July 1996, phytoplankton seemed to have a rather homogeneous biomass distribution in the southern Black Sea. In all periods, the coccolithophorid Emiliania huxleyi was the most abundant species, its contribution to the total abundance ranging from 73% in autumn to 43% in spring. However, in terms of biomass, diatoms made up the bulk of phytoplankton in spring (97%, majority being Proboscia alata) and autumn (73%, majority being Pseudosolenia calcar-avis), and dinoflagellates in summer (74%, Gymnodinium sp.). There was a remarkable similarity in the dominant species between the western and eastern regions of the southern Black Sea, indicating transport of phytoplankton within the basin.     

Seasonal variability of the Black Sea chlorophyll-a concentration

The optimal spectral decomposition (OSD) method is used to reconstruct seasonal variability of the Black Sea horizontally averaged chlorophyll-a concentration from data collected during the NATO SfP-971818 Black Sea Project in 1980–1995. During the reconstruction, quality control is conducted to reduce errors caused by measurement accuracy, sampling strategy, and irregular data distribution in space and time. A bi-modal structure with winter/spring (February–March) and fall (September–October) blooms is uniquely detected and accurately documented. The chlorophyll-a enriched zone rises to 15 m depth in winter and June, and deepens to 40 m in April and 35 m in August. The June rise of the chlorophyll-a enriched zone is accompanying by near-continuous reduction of upper layer maximum chlorophyll-a concentration.     

Variability of mesozooplankton spatial distribution in the North Aegean Sea, as influenced by the Black Sea waters outflow

The North Aegean Sea constitutes an important region of the Mediterranean Sea since in its eastern part the mesotrophic, low salinity and relatively cold water from the Black Sea (outflowing from the Dardanelles strait) meets the oligotrophic, warm and very saline water of Levantine origin, thus forming a thermohaline front. Mesozooplankton samples were collected at discrete layers according to the hydrology of the upper 100 m, during May 1997 and September 1998. In May highest biomass and abundance values (up to 66.82 mg m^− 3 and 14,157 ind m^− 3) were detected in the 10–20 m layer (within the halocline) of the stations positioned close to the Dardanelles strait. The front moved slightly southwards in September, characterized by high biomass and abundance values within the halocline layer. The areas moderately or non influenced by Black Sea water revealed lower standing stock values than the frontal area in both cruises and maxima were detected in the uppermost low salinity layer. Samples collected at the stations and/or layers more influenced by Black Sea water were distinguished from those collected at layers and/or stations more affected by Levantine waters in both periods. In May the former samples were characterized by the copepods Acartia clausi, Centropages typicus, Paracalanus parvus. The abundance of the above species decreased gradually with increasing salinity, in the horizontal and/or in the vertical dimension, with a parallel increase of the copepods Oithona plumifera, Oithona copepodites, Oncaea media, Ctenocalanus vanus, Farranula rostrata. During September the frontal area as well as that covered by the modified Black Sea water, were highly dominated by the cladoceran Penilia avirostris and doliolids. For both seasons, MDS plots, issued from the combination of mesozooplankton and water-type data, revealed the gradual differentiation of zooplankton composition from the frontal area towards the area covered by Levantine water, following the spreading and mixing of the Black sea water. The observed temporal and spatial variability in the distribution pattern of mesozooplankton standing stock and species composition seems to depend considerably on the variability of circulation and frontal flows.     

Egg production of the copepod Acartia hongi in Kyeonggi Bay, Korea

Many studies of copepod egg production have shown that food availability and temperature are major factors that influence copepod growth. However, coastal environments are complicated ecosystem and the relationships between growth of copepods and influencing factors are not always clear in nature. We conducted a study along an inner–middle–outer bay transect where variations in environmental parameters would be expected to affect the biomass and egg production rate of A. hongi from February 2001 to December 2001. In this study, we investigated the abundance and biomass with developmental stages and egg production rates of A. hongi in relation to various environmental factors. The copepod A. hongi occurred continuously throughout the year, with a peak abundance in May. In general, the variation in egg production rates showed a similar tendency with the variations in chlorophyll-a throughout the study period. This suggests that phytoplankton biomass is an important factor that affects the egg production of A. hongi. In addition, during the warm season, the egg production of A. hongi was also influenced by the ciliates abundance in the middle and outer bay. Consequently, the egg production of A. hongi is generally affected by food availability in Kyeonggi Bay.     

Surface chlorophyll in the Black Sea over 1978–1986 derived from satellite and in situ data

Absolute values of chlorophyll a concentration and its spatial and seasonal variations in the Black Sea were assessed by using satellite CZCS and in situ data. Since the satellite CZCS had operated for the 1978–1986 period, CZCS data was used for assessing the past state of the Black Sea just before the onset of drastic changes observed in late 1980s. The approach used for the calculation of the absolute values of chlorophyll a concentration from CZCS data was based on the direct comparison of in situ chlorophyll a data and those of CZCS and by applying the algorithm developed for the transformation of CZCS data into chlorophyll a values. CZCS Level 2 data related with pigment concentration having a spatial resolution of 1 km at nadir were used. The daily Level 3 files were derived by binning Level 2 values into 4-km grid cells and the monthly and seasonal Level 3 files were created by averaging the daily Level 3 files over the corresponding period. In situ chlorophyll a data were obtained by spectrophotometric and fluorometric methods in 15 scientific cruises over the 1978–1986 period. Total number of ship-measured data used for the comparison with those CZCS values was 590.Chlorophyll a concentration (Chl) was derived from CZCS values (C) with regression equations Chl=kC; the coefficient of transformation k was calculated from six different data sets by taking into account distinctions between subregions and seasons. The reasons for difference in the k values have been analyzed.Statistical comparison of the chlorophyll a values measured in situ and those derived from CZCS data was based on log-transformed data and gave the following results: regression SLOPE=0.842, regression INTERCEPT=−0.081, coefficient of determination (R²)=0.806, root–mean–square ERROR=0.195. The mean monthly chlorophyll a distributions derived from CZCS data over 1978–1986 have been constructed and the mean seasonal chlorophyll a values in different regions have been calculated and analyzed. The significant difference in chlorophyll concentration between the western shelf regions and the open part of the Black Sea has been demonstrated, especially in warm season. At almost all seasons, the highest chlorophyll concentration is observed in the western interior shelf region which is under strong influence of Danube. The summer mean chlorophyll concentration in this region is 18 times higher than that in the open parts and about nine times higher than in the eastern shelf region. The greatest seasonal variations are observed in the open part of the Black Sea: chlorophyll concentration in cold season is four to six times higher than in summer and three to five times higher than in April and October. To the contrary, in the western interior shelf regions, the concentration is higher in May–October (about twice than that in November–March). Seasonal variations in the western outer shelf regions are smoothed out as compared with both the western interior shelf and the open regions.     

The trophic role of the tunicate Salpa thompsoni in the Antarctic marine ecosystem

During a repeat grid survey and drogue study carried out in austral summer 1994/95, the abundance and feeding activity of salps were estimated in the Lazarev Sea region from net tows and in situ measurements of gut fluorescence. Throughout the survey area, Salpa thompsoni accounted for >95% of the total salp stock while Ihlea racovitzai was consistently represented in very low abundances. Maximum densities of S. thompsoni, with ≈4000 ind. 1000 m⁻³, were recorded in the Marginal Ice Zone (MIZ) in December when chlorophyll-a concentrations were well below 1 mg m⁻³. A dramatic decrease in salp stock was observed at the beginning of January, when S. thompsoni virtually disappeared from the most productive area of the MIZ where chlorophyll-a concentrations had by then reached bloom levels of 1.5–3 mg (Chl-a) m⁻³. In situ grazing measurements showed that throughout the cruise S. thompsoni exhibited the highest ingestion rates per individual of any of the most abundant components of the grazing pelagic community, with maxima of ≈160 μg (pigm) ind. ⁻¹ d⁻¹. These feeding rates are 3 to 5 times higher than those previously obtained using in vitro incubations. The total daily consumption of the population of S. thompsoni varied from 0.3 to 108% of daily primary production. We suggest that competitive removal of food by S. thompsoni, rather than direct predation, is responsible for the low krill abundances generally associated with salp swarms.     

Role of filtering and biodeposition by Adamussium colbecki in circulation of organic matter in Terra Nova Bay (Ross Sea, Antarctica)

At Terra Nova Bay, the scallop Adamussium colbecki (Smith, 1902) characterises the soft and hard bottoms from 20 to 80 m depth, constituting large beds and reaching high values of density (50–60 individuals/m²) and biomass (120 g/m² DW soft tissues). To assess its role in the organic matter recycling in the coastal ecosystem, its filtering and biodeposition rates were evaluated in laboratory experiments during the austral summer 1993/94. Filtration rates, measured in a flow-through system, were calculated from the difference in particulate organic carbon (POC), nitrogen (PON) and chlorophyll-a (Chl-a) concentration in inflow and outflow water. Experiments were performed using natural sea water with POC, PON and Chl-a concentrations of about 450 μg/l, 90 μg/l and 2 μg/l, respectively. The biodeposition rate and the biochemical composition of the biodeposits were studied in order to detect how the organic matter is transformed through feeding activity of A. colbecki. At +1°C temperature, the average filtering rate was about 1 l h⁻¹ g⁻¹ (DW soft tissues) in specimens ranging in body mass from 2 to 3 g (DW soft tissues) and 6–7 cm long. The biodeposition rate in 3–8 cm long specimens, ranging from 0.4 to 5.7 g (DW soft tissues), was about 5.65 mg DW/g DW/day, leading to an estimate of Corg flux, through biodeposition by A. colbecki, of about 21 mg C m⁻² day⁻¹ at in situ conditions. Comparison between the biochemical composition of seston and biodeposits shows a decrease of the labile compounds, of the Chl-a/phaeopigments ratio in the biodeposits. The recorded C/N ratio decrease suggests a microbial colonisation in the biodeposits. This study suggests that Adamussium colbecki plays an important role in coupling the material fluxes from the water column to the sea bed, processing about 14% of total Carbon flux from the water column to the sediments, with an assimilation efficiency of 36%.     

Environmental causes of the fluctuations of red shrimp (Aristeus antennatus) landings in the Catalan Sea

The fluctuations of catches of the deep-sea shrimp Aristeus antennatus (Risso, 1816) were analysed in the 6 ports of Catalonia where production is concentrated, based on monthly landings from 1988 to 2004. The 6 selected ports produced 300 t of red shrimp in 2004, or 80% of the total production in Catalonia. The series for each port showed clear interannual variability, with peaks of production in the early 1990s and more recently from 2001 to 2003. The time periods of the monthly data series, estimated by frequency analysis based on Fourier transform, varied around 7–8 years in the four central ports and 12–13 years in the two northern ports. Additionally, the different curves were not in phase: even in nearby ports, the maximum production is observed in different years. Since the North Atlantic Oscillation (NAO) index is an excellent proxy for long-term series of environmental variables, we aimed to explore relationships between the fluctuation of the NAO index and A. antennatus landings in the Catalan Sea. The correlation between the mean annual NAO index and the annual catches in each port was positive and significant with some time lags (from 1 to 3 years). The existence of clear patterns linking the NAO with marine ecological processes has been demonstrated in many studies, but the underlying ecological mechanisms are far from being well understood. The variations in environmental parameters linked to the NAO may act on biological organisms at different levels (individual, population) through physiology (metabolic and reproductive processes) or through trophic relationships, including ecological cascade effects. We propose that NAO-induced environmental variability may enhance food supply to A. antennatus and hence strengthen the reproductive potential of particular year classes, which result in increased catches 1 to 3 years later, although other possible effects of environmental variability on the population dynamics of this species are worth investigating.     

Evidence of episodic changes in deep-sea mesozooplankton abundance and composition in the Levantine Sea (Eastern Mediterranean)

Mesozooplankton samples from the 4270-m deep Ierapetra Basin in the oligotrophic Levantine Sea have revealed a strong temporal variability in abundance and composition hitherto unknown for the deep sea pelagic zone. The phenomenon reflected by a survey in June 1993 as compared to January 1987 is assessed by (i) the increase of the mesozooplankton standing crop throughout the water column, (ii) the absence of a decline in mesozooplankton abundance with increasing depth below 1000 m, (iii) the outstanding dominance of two interzonal calanoid copepods at bathypelagic and abyssopelagic depths and (iv) a substantial faunal change due to the codominance of Calanus helgolandicus, which was found for the first time in the area. The underlying hydrographical factors are addressed in the light of the Eastern Mediterranean Transient (EMT). Apart from a possible long-term faunal change, the EMT may exaggerate episodic plankton blooms and surface-abyssopelagic coupling in space and time.     

Annual cycle of distribution of three ice-associated copepods along the coast near Dumont d'Urville, Terre Adélie (Antarctica)

In polar regions sea ice is a site of enhanced primary production during winter and provides important habitat for small grazers, such as copepods. We sampled zooplankton from the sea ice and water column throughout 2005, near Dumont d'Urville station (Terre Adélie, Antarctica). Three species of ice-associated copepods were found: two calanoid copepods Paralabidocera antarctica and Stephos longipes and the harpacticoid copepod Drescheriella glacialis. P. antarctica was the most abundant of the three and was closely associated with the sea ice during most of the year. This species had a one year life cycle with a probable over-wintering period in the sea ice as nauplii and a short copepodite phase in spring. Reproduction and spawning occurred in early summer. A comparison with two other populations (near Syowa and Davis stations) along the east coast of Antarctica showed that there was a temporal shift in the life cycles of the three populations, which was linked to variability in sea ice conditions. D. glacialis was the second most abundant copepod and was more common during the winter than during summer, indicating its preference for the sea ice habitat. In autumn, the presence of D. glacialis in the deeper part of the water column suggested that this species colonised the sea ice from the benthos. S. longipes was found only in the water column near Dumont d'Urville and was not very abundant. In Terre Adélie particular environmental conditions, such as the absence of a permanent sea ice zone throughout the year, a longer time of open water, strong katabatic winds and the presence of polynyas, have influenced both the abundance and distribution of the three common ice-associated copepods.     

Natural and anthropogenic hydrocarbons in the water column of the Ross Sea (Antarctica)

In the framework of the Italian Research Programme in Antarctica (PNRA) an oceanographic cruise was carried out between December 2000 and February 2001 in the Western Ross Sea. n-Alkanes and PAHs concentrations were analysed in sea-water samples collected at different depths at four sites (D, B, CA2, and CA). Vertical profiles of pressure, temperature, salinity and fluorescence were also performed in order to distinguish the water masses present in the sampling areas. n-Alkanes concentrations were consistent with those found in the same area during previous expeditions and showed higher values in the particulate due to the low temperatures. n-Alkanes profiles exhibited different features in the dissolved and particulate phases. The dissolved phase was characterised by a bimodal distribution with two maxima at C16 and C24 and prevalent compounds in the range C15–C32 whereas particulate was strongly dominated by long chain n-alkanes C23–C34 with C24 (Site B, CA and CA2) or C32 (Site B) as Cmax. CPI values and Pr/Ph ratios suggested a prevalent biogenic origin for aliphatic hydrocarbons. n-Alkanes vertical distributions were affected by the dynamic of the pack-ice melting and phytoplankton activity as well by the chemical–physical properties of water masses. Higher n-alkanes values were found in the High Salinity Shelf Waters (HSSW), lower n-alkanes values were found in the Antarctic Surface Waters (AASW) and Modified Circumpolar Deep Water (MCDW).The low PAHs concentration levels confirmed the still pristine character of the Antarctic environment. Neither particular trend in total PAHs concentrations along the water column and nor correlations with the most characteristic water masses of the investigated area of the Ross Sea were observed, except that total PAH higher concentrations were generally observed in correspondence of HSSW.     

Production of Acartia omorii (Copepoda: Calanoida) in Ilkwang Bay, southeastern coast of Korea

Production of the marine calanoid copepod Acartia omorii was measured from 2 October 1991 to 8 October 1992 at a station in Ilkwang Bay on the southeastern coast of Korea. A. omorii (nauplii + copepodites + adults) were present in the plankton throughout the year, with seasonal variation in abundance. Biomass of A. omorii was averaged at 0.44 mgC m^− 3, with peaks in February and July, and relatively low biomass in late summer and fall. Egg production rate ranged from 2.4 to 151.9 μgC m^− 3 day^− 1, which was equivalent to 95–6075 eggs m^− 3 day^− 1. Fecundity of an adult female was averaged at 38 eggs female^− 1 day^− 1. Instantaneous growth rates of copepodites were higher than those of nauplii stages. Annual production of A. omorii ranged from 33.5 mgC m^− 3 year^− 1 to 221 mgC m^− 2 year^− 1, showing a seasonal variation of daily production rate with peaks in February and July. The daily production rate of A. omorii was significantly correlated with chlorophyll a concentration. These results suggest that standing stocks and/or productivity of phytoplankton are the major influencing factors, rather than water temperature for the seasonal variation of production of A. omorii in Ilkwang Bay.     

Effects of closed trawl fishery areas on some elasmobranch species in the Cantabrian Sea

This study examines the effects of certain closed areas to the trawl fishery on some Rajidae species and small-spotted catshark in the southern area of the Bay of Biscay. The results are based on abundance index (number/haul) and catch length composition obtained from a series of bottom trawl surveys carried out in the area (1993–2005). Abundance trends are similar considering or not closed areas, but with much lower values excluding closed areas. The length distributions revealed that in some of the species like Scyliorhinus canicula the larger specimens were the most affected by this decrease in number. Finally, the effects and consequences of one of these marine protected areas (MPAs), an artificial reef were examined in more detail for all the fishes and invertebrates. The analysis of the yield series showed an increase in total biomass after the placement of the reefs not only for S. canicula and rays but also teleosts fishes like Pagellus acarne, Trachurus trachurus, Mullus surmuletus and Trachinus draco and particularly invertebrates (sea urchins). On the contrary, the main target species of the trawl fishery (hake, monkfish and megrim among others) did not increase.     

pH variability and CO2 induced acidification in the North Sea

A coupled carbonate system–marine ecosystem–hydrodynamic model is used to simulate the temporal and spatial variability in pH across the southern North Sea as it relates to the environmental and biological processes affecting CO₂, namely, photosynthesis and respiration, riverine boundary conditions and atmospheric CO₂ concentrations. Annual pH ranges are found to vary from < 0.2 in areas of low biological activity to > 1.0 in areas influenced by riverine signals, consistent with observations and previous studies. It is shown that benthic, as well as pelagic, activity is an important factor in this variability. The acidification of the region due to increased fluxes of atmospheric CO₂ into the marine system is calculated and shown to exceed, on average, 0.1 pH units over the next 50 years and result in a total acidification of 0.5 pH units below pre-industrial levels at atmospheric CO₂ concentrations of 1000 ppm. The potential for measurable changes in biogeochemistry are demonstrated by simulating the observed inhibition of pelagic nitrification with decreasing pH. However, we conclude that there is a lack of knowledge of how acidification might affect the complex interaction of processes that govern marine biogeochemical cycles and a consequent need for further research and observations.     

Validation of the 3D biogeochemical model MIRO&CO with field nutrient and phytoplankton data and MERIS-derived surface chlorophyll a images

This paper presents results obtained with MIRO&CO-3D, a biogeochemical model dedicated to the study of eutrophication and applied to the Channel and Southern Bight of the North Sea (48.5°N–52.5°N). The model results from coupling of the COHERENS-3D hydrodynamic model and the biogeochemical model MIRO, which was previously calibrated in a multi-box implementation. MIRO&CO-3D is run to simulate the annual cycle of inorganic and organic carbon and nutrients (nitrogen, phosphorus and silica), phytoplankton (diatoms, nanoflagellates and Phaeocystis), bacteria and zooplankton (microzooplankton and copepods) with realistic forcing (meteorological conditions and river loads) for the period 1991–2003. Model validation is first shown by comparing time series of model concentrations of nutrients, chlorophyll a, diatom and Phaeocystis with in situ data from station 330 (51°26.00′N, 2°48.50′E) located in the centre of the Belgian coastal zone. This comparison shows the model's ability to represent the seasonal dynamics of nutrients and phytoplankton in Belgian waters. However the model fails to simulate correctly the dissolved silica cycle, especially during the beginning of spring, due to the late onset (in the model) of the early spring diatom bloom. As a general trend the chlorophyll a spring maximum is underestimated in simulations. A comparison between the seasonal average of surface winter nutrients and spring chlorophyll a concentrations simulated with in situ data for different stations is used to assess the accuracy of the simulated spatial distribution. At a seasonal scale, the spatial distribution of surface winter nutrients is in general well reproduced by the model with nevertheless a small overestimation for a few stations close to the Rhine/Meuse mouth and a tendency to underestimation in the coastal zone from Belgium to France. PO₄ was simulated best; silica was simulated with less success. Spring chlorophyll a concentration is in general underestimated by the model. The accuracy of the simulated phytoplankton spatial distribution is further evaluated by comparing simulated surface chlorophyll a with that derived from the satellite sensor MERIS for the year 2003. Reasonable agreement is found between simulated and satellite-derived regions of high chlorophyll a with nevertheless discrepancies close to the boundaries.     

Seasonality of picophytoplankton chlorophyll a and biomass in the central Cantabrian Sea, southern Bay of Biscay

Seasonal changes in the abundance and biomass of cyanobacteria (Synechococcus and Prochlorococcus) and picoeukaryotes were studied by flow cytometry in the upper layers of the central Cantabrian Sea continental shelf, from April 2002 to April 2006. The study area displayed the typical hydrographic conditions of temperate coastal zones. A marked seasonality of the relative contribution of prokaryotes and eukaryotes was found. While cyanobacteria were generally more abundant for most of the year (up to 2.4 10⁵ cells mL^− 1), picoeukaryotes dominated the community (up to 10⁴ cells mL^− 1) from February to May. The disappearance of Prochlorococcus from spring through summer is likely related to shifts in the prevailing current regime. The maximum total abundance of picophytoplankton was consistently found in late summer–early autumn. Mean photic-layer picoplanktonic chlorophyll a ranged from 0.06 to 0.53 µg L^− 1 with a relatively high mean contribution to total values (33 ± 2% SE), showing maxima around autumn and minima in spring. Biomass (range 0.58–40.16 mg C m^− 3) was generally dominated by picoeukaryotes (mean ± SE, 4.28 ± 0.27 mg C m^− 3) with an average contribution of cyanobacteria of 30 ± 2%. Different seasonality of pigment and biomass values resulted in a clear temporal pattern of picophytoplanktonic carbon to chlorophyll a ratio, which ranged from 10 (winter) to 140 (summer). This study highlights the important contribution of picoplanktonic chlorophyll a and carbon biomass in this coastal ecosystem.     

Vertical profiles of particulate organic matter and its relationship with chlorophyll-a in the upper layer of the NE Mediterranean Sea

Particulate organic matter (POM), nutrients, chlorophyll-a (CHL) and primary production measurements were performed in the upper layer of three different regions (cyclonic, anticyclonic and frontal+peripherial) of the NE Mediterranean Sea in 1991–1994. Depth profiles of bulk POM exhibited a subsurface maximum, coinciding with the deep chlorophyll maximum (DCM) established near the base of the euphotic zone of the Rhodes cyclone and its periphery, where the nutricline was situated just below the euphotic zone for most of the year. Moreover, the POM peaks were broader and situated at shallower depths in late winter–early spring as compared to its position in the summer–autumn period. Under prolonged winter conditions, as experienced in March 1992, the characteristic POM feature disappeared in the center of the Rhodes cyclone, where the upper layer was entirely occupied by nutrient-rich Levantine deep water. Deep convective processes in the cyclonic gyre led to the formation of vertically uniform POM profiles with low concentrations of particulate organic carbon (POC) (2.1 μM), nitrogen (0.21 μM), total particulate phosphorus (PP) (0.02 μM) and chlorophyll-a (0.5 μg/L) in the euphotic zone. Though the Levantine deep waters ascended up to the surface layer with the nitrate/phosphate molar ratios (28–29) in March 1992, the N/P molar ratio of bulk POM in the upper layer was low as 10–12, indicating luxury consumption of phosphate during algal production. Depth-integrated primary production in the euphotic zone ranged from 38.5 for oligotrophic autumn to 457 mg C m⁻² day⁻¹ for moderately mesotrophic cool winter conditions.