Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.     

热再生预制构件在高速公路附属工程中的应用研究

依托实际工程,将高速公路路侧急流槽热再生预制构件预制安装工艺与传统施工工艺进行优缺点对比分析,并结合其在排水沟、路缘石等附属工程中的应用,得出该技术具有预制安装方便快捷、耐久性能好的特点。同时,其拓展了再生技术的应用领域,为沥青铣刨料的回收再利用提供了新思路。