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济南铁路局职业危害状况及演变趋势 总被引:2,自引:1,他引:1
对济南铁路局1984年~1995年177名职业病发病报告资料整理分析,结果显示,12年中共诊断职业病7类16种,发病率为0.23%,表现为1989年前呈逐年下降趋势,1989年后呈逐年上升趋势,平均发病年龄及工龄分别为38.6和12.3,职业危害以尘肺和职业中毒为主,工种以装卸工、凿岩工、电焊工为前三位。 相似文献
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Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells (RPCs) in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 + N2 with EGF and bFGF (suspension medium ) or 10 % FBS without EGF and bFGF ( differentiation medium). The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin (92.86%) or Pax6 (86.75%), Map2 (38.54%), GFAP (20.93%), Thy-1 (27. 66%) and Rhodopsin(13. 33%)in suspension medium; however, Nestin (60.27%), Pax6 (52%), Map2 (34.94%), GFAP (38.17%), Thy-1 (30.84%) and Rhodopsin (34.67%) in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types. 相似文献
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隧道岩溶富水区帷幕注浆设计 总被引:8,自引:0,他引:8
针对隧道岩溶富水区涌水量大、压力高等特点,全隧按照“以堵为主,限量排放”的原则对高压富水区进行帷幕注浆堵水设计。 相似文献
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目的探讨β-淀粉样蛋白25-35(Aβ25-35)与谷氨酸(Glu)联合应用,对原代培养的大鼠基底前脑神经元的作用。方法原代培养大鼠基底前脑神经元,应用形态学、MTT法结合ChAT免疫组织化学染色,分别观察Aβ25-35与Glu联合应用,对原代培养的基底前脑神经元存活率、形态学及ChAT免疫反应阳性神经元的影响。结果将100 nmol/L、1μmol/L Aβ分别和20μmol/L Glu联合用于培养的基底前脑神经元,在倒置显微镜下观察,细胞表面粗糙,立体感减弱,胞浆中出现深色颗粒,细胞肿胀或收缩;尼氏染色显示尼氏体减少;MTT检测显示神经元存活率明显下降,100nmol/L Aβ+20μmol/L Glu组与100 nmol/L Aβ组间,1μmol/L Aβ+20μmol/L Glu组与1μmol/L Aβ组间比较均具有统计学差异(P<0.05);ChAT免疫阳性神经元的灰度和截面积均减小。结论Aβ25-35可增加神经元对Glu神经毒性作用的敏感性,表明Glu在阿尔茨海默病发病中起着非常关键的作用。 相似文献
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盾尾注浆可以有效地提高隧道的防水质量,但浆液配合比设计困难,对浆液基本特性分析后,通过多次试配确定设计配合比,并在实施过程中对配合比进行优化。使盾尾同步注浆顺利实施。 相似文献
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采用变形叠加方法分析了柔性微动关节的受力(矩)变形位移过程,推导了柔性关节两端广义力传递的矩阵变换.以对称半圆弧曲线柔性关节为例进行分析,并与以往采用伪刚体方法进行运动学建模的分析结果相比较,其结果与有限元分析结果更接近,从而大大提高了模型精度. 相似文献