单细胞凝胶电泳技术检测小鼠成纤维细胞DNA的氧化损伤及修复

目的观察小鼠成纤维细胞由过氧化氢(H2O2)引起的DNA损伤及其修复,并详细介绍单细胞凝胶电泳技术。方法培养NIH3T3细胞,H2O2造成细胞氧化损伤,单细胞凝胶电泳技术(SCGE,Comet Assay)检测细胞DNA的损伤情况。结果①建立了H2O2致NIH3T3细胞DNA损伤的分级图谱;②H2O2引起的小鼠成纤维细胞DNA单链断裂与H2O2的浓度呈依赖性关系;③细胞在除去H2O2后15 min已出现明显修复,多数修复可在1 h内完成,但少数修复可能需要较长时间才能完成。结论单细胞凝胶电泳技术是一种简便、敏感的检测DNA氧化损伤的方法。     

Research on the dynamic performance of low-yield-strength shear panel damper for bridge application

This study seeks to develop a scientific understanding of the effects of dynamic loading history on the low-yield-strength shear panel damper(LYSPD) mechanical properties and fatigue performance.The LYSPD model is supposed as perfect elastic-plastic without strength deterioration.By adjusting the damper strength with the above supposition in the DYMO software,the serial earthquake response waves of the LYSPD under different soil conditions are obtained firstly.Subsequently,several waves,the most prone to damage at each soil condition, are selected as dynamic loading waves to verify the LYSPD dynamic performances experimentally.The test results suggest that the dynamic loading histories have no influence on the LYSPD strength or the supposed model while they affect the fatigue performance.The applicability and accuracy of three fatigue evaluation methods, cumulative plastic shear strain,cumulative energy absorption and Miner rule,are also compared according to the test results.     

使用振动能量传播解析来减少振动的两个阶段设计

正1前言机械产品的静穆性是重要的产品价值,随着今后进一步的电动化,应该处理的频带就变得更有高度,更加广阔。振动噪音问题的解决虽然是以回避共振为出发点的,但是对于宽频带问题也需要下很大功夫。现在我们举个例子,图1显示的是两端单纯支撑边梁的驱动点的加速度按照运动的方程式算出的结果(EOM,细线)和无限长梁根据波动解析