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Highly porous ultrafine electrospun scaffolds, gelatin/poly(L-lactic acid) (PLLA) and chitosan/PLLA were prepared by blending gelatin and PLLA, chitosan and PLLA respectively. The biocompatibilities of these scaffolds were assessed by attachment, proliferation and viability of cells on them. The results indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding, while the attachment of the cells was only 15% on PLLA scaffolds. Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells. Cell growth on the gelatin/PLLA and chitosan/PLLA scaffolds showed dramatic improvement, indicating a much better biocompatibility in the blends contributed by gelatin and chitosan. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells. These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering. 相似文献
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为提高我国空中交通管制(简称"空管")的服务质量,弥补现有空管服务质量评价指标体系在构建上的局限性,在原体系的基础上进一步完善了我国空管服务质量评价指标体系。首先,从空管服务过程分析了影响空管服务质量的主要因素。然后,基于质量管理体系中的过程方法和戴明循环理论,建立空管服务质量管理模型。接着,以该模型为基础,选取运行安全、运行效率、管理效能和文化建设四个关键指标,构建了空管服务质量评价指标体系。最后,以厦门空管站的空管服务评价体系指标设置为例,对所建指标体系进行验证。结果表明,该评价体系具有一定的客观性和科学性。 相似文献
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城市轨道交通大跨度小半径曲线梁桥设计 总被引:1,自引:0,他引:1
轨道交通大跨度小半径曲线梁桥具有受力复杂、车辆荷载大、设计难度高等特点。曲线梁桥由于轴向变形与平面内弯曲耦合,竖向挠曲与扭转耦合,以及弯曲、扭转与截面畸变耦合等因素,易发生梁体水平径向位移过大、梁体翘曲、墩梁固结处开裂、支座脱空等工程问题。依托大连轻轨某小半径大跨度预应力混凝土曲线梁桥工程,通过对曲线桥梁受力情况的分析,考虑变形耦合效应,确定了截面、钢筋及预应力钢束设置等关键技术参数,并针对曲线梁存在的"外梁超载,内梁卸载"问题,对支承形式及偏心等关键参数进行了讨论。研究表明,采用单箱单室截面、合理设置钢筋、预应力约束、支座等措施能有效解决此类工程问题。 相似文献