Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

水泥砼路面错台快速修补材料的研究

采用正交设计的方法对快硬硫铝酸盐水泥和聚合物丁苯乳液进行配合比试验,确定出最佳水泥砼配合比,通过路用性能对比试验和耐久性对比试验表明,这种有机、无机多相复合的新型道路水泥砼能有效的弥补普通砼的缺点,具有优良的力学和路用性能:早期强度高,养护12 h即可开放交通,干缩小,耐久性能好,与旧路面之间层间黏结强度高,并且在错台高度仅为0.6 cm时,模拟错台修补效果很好,没有出现脱落和开裂.     

现代有轨电车安全运行的危险源识别与风险评估

随着现代有轨电车的快速发展,运行安全问题日益凸显。针对现代有轨电车运行安全问题,从系统外部和系统内部分析其安全运行危险因素,建立危险因素库。运用事故树法,根据各个基本事件的逻辑关系搭建事故树,识别危险源,确定事故发生原因。基于LEC法赋值3种因素指标,进行风险评估,确定风险等级。分析结果表明交通环境差、机非混行、乘客的不安全行为以及驾驶员操作不当是造成事故发生的主要危险源。自然灾害、驾驶员操作不当以及共享路权导致事故后果严重,风险等级高。根据结果提出相应的风险防控措施,保证车辆的安全运行。     

滑框倒模技术在空心薄壁高墩中的应用

介绍了京福高速公路福建段上洋大桥空心薄壁高墩中采用的滑框倒模技术,该技术结合了滑模和翻模的优点,在保证工程施工进度、质量的同时,还较好地满足了墩身外观质量的要求.根据滑框倒模的施工特点和施工效果,对这一技术提出了其应用前景.     

EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFTER SPINAL INJURY IN ADULT RATS

Objective To study the expression of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were created by artery clamp. Expression of Nestin and GFAP in different period ( 1,3,5days; 1-8 weeks) and different area( injury locus and its surrounding tissue ) after spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5001W imaging processing system.Results There was expression of Nestin and GFAP in injured area 1 day after injury. The expression increased not only in in injured area but its sourrounding 3--7 days later and gradually got to peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between expression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell maybe invovled in the repair of central nervous system (CNS).     

智能交通系统中的智能车辆运输技术

分析了智能交通系统中的智能车辆运输系统，并重点分析了其中的车辆定位、车辆调度、通讯、自动驾驶等关键技术的现状和发展趋势。最后提出了智能车辆敏捷运输系统的结构。     

公路设计与仿真协同框架

公路设计过程是多学科的综合应用及设计人员创造性思维的过程.计算机仿真技术可以通过建立不同层次的仿真模型,对设计进行不同深度的评价.笔者从公路设计的特点出发,探讨了公路仿真技术在公路CAD系统的应用特点和公路设计对仿真系统的需求,进而构造了公路设计仿真协同框架,为仿真技术与设计技术的结合奠定了基础.     

路面结构优化遗传算法研究

遗传算法是建立在自然遗传学机理基础上的参数搜索方法.利用遗传算法对路面结构进行优化,可成功求解沥青路面结构非线性优化设计模型,具有节省时间、优化结果准确的特点.其研究成果对改善路面设计方法,降低工程造价,避免和减少工程病害有积极的作用.     

一种结构安全监测及远程数据采集系统的设计

提出一种基于TMS320LF2407和以太网的结构安全监测及远程数据采集系统,分析了系统的整体方案,对系统硬件设计进行了详尽的介绍,并就A/D转换及网络通信软件设计时应注意的关键问题进行了探讨,本系统解决了数据采集卡与以太网接口并进行远程数据传送问题,通过在Labview平台下对数据进行实时显示、查询、分析等功能,有利于寻找大型结构振动-安全规律,为测控系统向网络化发展指明了方向.可用于桥梁、大坝等大型结构的健康监测.