动车组运行状态智能检测装备

动车组运行状态智能检测装备设置于动车段入库线上,主要针对动车组走行部、车顶和受电弓在运用中出现内部缺陷、磨损、损坏及尺寸超限的故障比率问题。实时采集运行列车的底部、侧部和顶部图像,采用故障自动识别策略,对列车的车底走行部、闸瓦、转向架、接触网等与列车有关的各个部件进行动态监控。根据实验和现场使用情况,本检测设备满足铁路机车运行时对走行部和受电弓进行在线测量检测的要求,其中,走行部检测精度可达1 mm,滑板磨耗值测量精度可达0.2 mm。鉴于此,该动车组智能检测装备能及时发现故障隐患,为检修和更换提供依据,保证动车组运行安全。     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

君王 凯迪拉克SLS

享受美式奢华，CTS还不够？那么SLS或许能满足你的虚荣，这款针对主流豪华车市场的大家伙是凯迪拉克的旗舰产品，它的招摇可不只一点点！[编者按]     

不起眼的贵族 07款Audi A8L 4.2FSI

在中国，奥迪的旗舰很郁闷，和国产的A6L长的太像，这使A8L总是让人觉得不起眼，但是有品位的人对此并不介意，因为这台身价百万的车子其实是位低调的贵族。[编者按]     

韩式RV：双龙享御KYRON

双龙KYRON的名字由“Kai＋Run”变形而来，Kai起源于拉丁语，意为“无限”；Run则是奔跑的意思。“Kai-Run”寓意“无限疾驰，自在飞奔”。享御的诞生标志着韩国双龙这家专业的SUV厂商正向着RV方向努力。[编者按]     

冰雪华尔兹 与MERCEDES-BENZ 4MATIC共舞

跟着你的舞步,和着我的节拍。我们在北国风光中,映着千里冰封、万里雪飘,舞起一曲冰雪"华尔兹",在波浪起伏中接连不断旋转,如行云流水般顺畅,潇洒自若,典雅大方。     

基于误差平方和极小化的多模型组合预测研究

以某港口1997-2007年集装箱吞吐量为原始数据,建立了回归分析、3次指数平滑及灰色系统方法的港口集装箱吞吐量单项预测模型。鉴于单项预测模型的局限性,以误差平方和极小化为最优化准则,进行了多模型组合预测,并辅以实例进行分析和验证,通过误差的分析表明该模型能够改善预测精度,可以作为未来港口集装箱吞吐量预测的工具。     

高峰时段铁路车站晚点时长分布规律

基于运行实绩对高铁车站在早晚高峰时间段下的晚点时长分布规律进行研究，为行车人员提供行车指导，辅助行车人员充分估计晚点时长特征及预测晚点发展态势，更好地进行调度决策。首先描述高速铁路沪昆线上的运行实绩数据，并统计出早晚高峰时段下的车站晚点时长数据。然后，结合统计模型构建车站在早晚高峰时间段下的晚点时长分布模型，并比选确定出最优的晚点时长分布模型。最后，对车站在早晚高峰时段下的晚点时长分布特征进行归纳总结，相关研究结论能为列车晚点传播机理以及智能高铁调度指挥系统提供基础支撑。