从限界角度分析北京地铁5号线采用国家地铁标准B型车的可行性

本从限界角度，分析了目前施工设计阶段，地铁5号线各区间隧道、车站断面以及原来预留好的隧道断面条件下，由车高3．7m的现有北京鼓形车改为国家地铁标准B型车的可行性。     

Serum levels of RBP4 might not be determined by diabetes mellitus but by kidney function and renal replacement therapy

It has been reported that retinol-binding protein 4(RBP4) is associated to adiposity,insulin resistance,and type 2 diabetes.Meanwhile,circulating RBP4 levels are also affected by renal function.The aim of the present study is to investigate whether serum levels of RBP4 are primarily associated with different stages of chronic kidney disease(CKD) or type 2 diabetes,if there is more potential relevance between RBP4 and renal replacement therapy.The serum levels of RBP4 were assessed by commercial competitive enzyme-linked immunosorbent assay(ELISA)kit in 212 patients with the CKD stages 1—5 and in 24 healthy controls,while its correlation with clinical and metabolic parameters was analyzed.The serum level of RBP4 had a strong correlation with estimated glomerular filtration rate(e GFR)(P 0.001).Stratified by e GFR and treatment,no more differences in RBP4 serum concentration were detected between type 2 diabetic and non-diabetic subjects [CKD stages 1—5,non-dialysis(ND),hemodialysis(HD) and peritoneal dialysis(PD);P 0.05 for all].The elevation of RBP4 become higher in HD than in PD and ND in CKD5 patients(P = 0.008 and P = 0.04,respectively),while there was no significant difference between PD and ND groups.Multivariate linear regression analysis demonstrated three independent predictors of e GFR(β =-0.676,P 0.001),C-reactive protein(CRP)(β =-0.573,P 0.001) and creatine(β = 0.509,P = 0.024) in the study population.The study results demonstrated that the serum level of RBP4 was negatively related to the e GFR,whether diabetes mellitus(DM) affected the blood concentration of RBP4 or not.And the serum level of RBP4 exhibited significant difference in different renal replacement therapies.     

运河航道开发过程中生态带建设技术方案

针对运河护岸经常被破坏的情况,通过研究运河航道中船行波对岸坡的作用及破坏机理,分析不同形式生态护坡的消波能力,研究运河航道多功能协调的护坡关键技术。总结航道生态护坡设计原则和思路,提出生态护坡结构应尽量设置多孔性结构,为生物提供生长空间。护坡结构形式选择要综合考虑水岸相互作用和生态需求。在船舶通行密度高的航道,船舶航行会对水生生物造成重大影响,有条件时应单独开辟一块区域供水生生物栖息。     

农村单车道公路错车道设置参数研究

以农村公路人和车辆安全为出发点,对错车道设置条件、长度、宽度、间距及渐变段长度和宽度进行了研究,综合考虑农村单车道公路车型分布、行驶车速、交通量等因素,推导出错车道设置长度、宽度、间距及渐变段长度的计算公式.以福建省某地区的农村公路为实例,证明提出的错车道设置参数是合理的.     

Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein

Renalase is a secreted amine oxidase that metabolizes catecholamines. It has been proposed to modulate blood pressure and heart rate and its downregulation might result in hypertension. Despite its potential relevance for human health, the biochemical characterization of renalase is still scarce. The aim of this study is to synthesize the human renalase eukaryotic protein by genetic engineering. The human renalase gene was amplified by polymerase chian reaction (PCR). After digestion by BamH I and Xho I enzymes, the DNA fragments were cloned into the transfer vector, pFastBacHTb-Fc, to generate the pFastBacHTb-renalase expression vector. The ligation products were transformed into E. coli DH10Bac to obtain recombinant transposon rBacmid-renalase. The recombinant transposon was further transferred into insect high-V cells, and the recombinant human renalase eukaryotic protein was expressed successfully.     

基于数值试验的减振器阻抗特性研究

基于四端参数法分析了计及高频波动效应的圆柱型橡胶减振器的阻抗特性,以E型和BE型减振器为研究对象,通过合理的建模技术,借助有限元软件数值计算该减振器的机械阻抗。在此基础上,探索加载对减振器机械阻抗的影响规律,所得的计算结果与减振器的实测特性相符。该文为后期减振器的研制及其阻抗特性的确定提供计算方法。     

基于用户需求把握的轻型商用车产品展示

本文深入研究轻型商用车用户的核心需求,建立了"货、车、人"三维分析模型,并选取地理环境、年龄结构等六项分析要素进行用户特征画像,并以此模型为基础,探讨轻型商用车产品展示的关键模式和环节,提出了感官式绕车六步介绍法,促进了销售的达成和品牌的维护。     

DF8B型内燃机车电空电器电磁化改造

针对DF_(8B)型内燃机车电器柜电空电器故障率高、可靠性差、结构笨重等问题,提出了解决此类问题的总体思路和办法,取得了良好的效果。     

高专教育课程考试改革探讨

通过分析现行高专教育课程考试制度中存在的问题，按新世纪高等教育的要求，提出改革课程考试的相应措施。     

浅析理实一体化教学法在《汽车维护》课程中的运用——以更换机油及滤清器为例

正理实一体化教学法将理论与实践教学有机结合,通过理论指导实践、实践验证理论,理论和实践交替,实现知识和技能传授,更具针对性、合理性、实用性。更换机油及滤清器是《汽车维护》课程中一个重要的学习任务,用6课时来完成,根据教学内容及学情分析,将整个教学过程分为情景导入等6个环节来完成。其中情景导入、微课学习用时0.5课时,实践操作2课时,选拔竞技2课时,总结评价1课时,检验提升0.5课时。