Genome-wide profiling identifies Saccharomyces cerevisiae response in PKC-SLT2 signaling and glycogen metabolic pathways to antifungal compound calcofluor white

Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white （CFW） is an attractive antifungal compound for its known inhibitive effects on trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have used microarrays to examine the cell＇s response when treated with CFW on the genome scale. We found that both the PKC-SLT2 （i.e, protein kinase C-mitogen activated protein kinase） and the glycogen metabolic pathways are activated upon CFW treatment. These results suggest that the key components in these pathways could be targeted by other drugs to counter the cell＇s compensative response, thus to further substantiate the inhibitive effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound in clinical applications.     

ATO熔断片过渡端子的改进

车用电线束插接器端子是插接器电力和信号传输性能的关键零部件,其结构设计是决定连接器端子性能的一个重要因素。本文通过对原ATO熔断片过渡端子结构弊端的分析,优化设计一种新型的ATO熔断片过渡端子。     

软弱围岩隧道开挖方案优化研究

根据合福线合肥至福州段的万山隧道特点和隧道开挖施工现状,参考前期围岩变形、锚杆应力等监测成果,采用有限元方法分别模拟万山隧道Ⅴ级围岩六步CD法和三台阶七步法两种开挖方案,比较分析两种开挖方案下洞室应力、变形的数值结果,并结合结构安全、稳定情况,论证三台阶七步法施工方案的可行性和合理性。     

桥梁冬季施工的研究与探讨

结合桥梁冬季施工中存在的问题,对水泥水化热的影响和混凝土早期受冻的影响等问题进行详细介绍.以贵阳某桥段冬季施工为例,针对所出现的问题采取了材料保温与养护保温的改善措施,可为同类工程提供参考借鉴.     

Preparation and Characterization of the Covalent-Integrated Poly（lactic acid） and Scrap Leather Fiber Composites

Scrap leather fibers (SLFs) modified with active silane containing epoxide groups were incorporated into poly(lactic acid) (PLA) modified by vinyl trimethoxysilane, i.e., the PLA/SLF composites were prepared for bio-composites by solvent compounding technology in this article. The effects of silane coupling agents on the structures of PLA and SLF molecules were examined, and the mechanical properties and thermal stability of PLA/SLF composites were also measured. The results of Fourier-transform infrared spectroscopy (FTIR) had proved that silane had been incorporated successfully to PLA and SLF molecules by means of the solvent technology. Scanning electric microscopy (SEM) was used to investigate both the changes in shapes between the pristine SLFs and the treated SLFs and the microscopic structures of composites. According to SEM results, it was shown that there were some significant differences between the untreated and treated SLFs, and a double continuous phase structure had occurred in PLA/SLF composites due to the excellent dispersion of SLFs in matrix. The addition of the treated SLFs into PLA resulted in a distinct improvement of the impact and tensile strengths. When the mass fraction of the treated SLFs was 15%, the notched impact strength and tensile strength of PLA/SLF bio-composites were improved by 34.4% and 21.2% compared with the pristine PLA, respectively. Additionally, with the increase of the modified SLFs content, the thermal stability of PLA/SLF bi-composites was apparently improved. The macroscopic properties of bio-composites were found to be strongly dependent on their components, concentration, dispersion and resulted morphological structures.     

论沥青混凝土路面裂缝的产生原因及防治措施

对沥青混凝土路面裂缝产生的原因进行分析,提出相应的防治措施。     

AutoCAD使用及二次开发

1 AutoCAD使用中的问题及解决方法AutoCAD作为设计行业的绘图软件,其操作简单易用,应用面之广泛在诸多软件中可以说是首屈一指的。AutoCAD版本也由原先AutoCAD R14升级至AutoCAD R2007,版本的升级带来了诸多新功能和便利,但是R14这     

信号交叉口专用双左转车道交通特性分析

结合北京市典型信号控制交叉口实际情况,现场调查高峰时段7个保护相位下专用双左转车道的车头时距、周期流量、大型车比例等数据,对双左转车道使用特性和调头车辆影响特性进行分析。结果表明,左转车辆的内、外侧车道利用率为50%,大型客车倾向于选择外侧左转车道行驶,给出双左转饱和流率的范围为1587～1818pcu/h/ln,平均值为1665pcu/h/ln,并且得到内、外侧左转饱和车头时距均值无显著性差异的结论。考虑到内侧车道特有的调头行为对左转车辆运行的影响,用数学方法确定了调头行为带来影响的范围,给出左转调头车辆相对于左转标准小型车的当量值为1.39。     

城市快速路施工区通行能力研究

在实测数据的基础上,对快速路施工区交通运行特性进行了分析,研究了施工区交通组成、饱和流率、车头时距、运行速度等交通参数分布特性,得到了城市快速路施工区通行能力值介于1500veh/h/ln～1700veh/h/ln之间。应用计算机仿真技术,给出了双向8车道快速路单向不同车道关闭形式下的施工区通行能力值。最后分析了施工区的速度、流量和密度之间的关系,给出了拥挤排队状态下速度与流量之间的函数关系式。     

设计时速与直行车道数对饱和流率的影响分析

信号交叉口作为城市道路的重要组成部分,其饱和流率受众多因素的影响。以美国通行能力手册中饱和流率模型为基础,通过对北京市典型信号交叉口车头时距的现场测定,对设计时速与直行车道数对饱和流率的影响进行研究,可完善饱和流率修正模型。